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Protein

Penicillin-binding protein 1B

Gene

mrcB

Organism
Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei203 – 2031Proton donor; for transglycosylase activityUniRule annotation
Active sitei481 – 4811Acyl-ester intermediate; for transpeptidase activityUniRule annotation

GO - Molecular functioni

  1. penicillin binding Source: InterPro
  2. peptidase activity Source: InterPro
  3. peptidoglycan glycosyltransferase activity Source: InterPro

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  3. regulation of cell shape Source: UniProtKB-KW
  4. response to antibiotic Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosyltransferaseUniRule annotation, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradationUniRule annotation, Peptidoglycan synthesisUniRule annotation

Enzyme and pathway databases

BioCyciHDEF572265:GJAB-868-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1BUniRule annotation
Short name:
PBP-1bUniRule annotation
Short name:
PBP1bUniRule annotation
Alternative name(s):
Murein polymeraseUniRule annotation
Gene namesi
Name:mrcBImported
Ordered Locus Names:HDEF_0862Imported
OrganismiHamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT)Imported
Taxonomic identifieri572265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeaphid secondary symbiontsCandidatus Hamiltonella
ProteomesiUP000002334 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: UniProtKB-KW
  2. peptidoglycan-based cell wall Source: InterPro
Complete GO annotation...

Interactioni

Protein-protein interaction databases

STRINGi572265.HDEF_0862.

Family & Domainsi

Sequence similaritiesi

In the C-terminal section; belongs to the transpeptidase family.UniRule annotation
In the N-terminal section; belongs to the glycosyltransferase 51 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0744.
KOiK05365.
OMAiIAQVWVT.
OrthoDBiEOG6HMXCD.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011813. PBP_1b.
IPR001460. PCN-bd_Tpept.
IPR028166. UB2H.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
PF14814. UB2H. 1 hit.
[Graphical view]
PIRSFiPIRSF002799. PBP_1b. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsiTIGR02071. PBP_1b. 1 hit.

Sequencei

Sequence statusi: Complete.

C4K4T9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHYGHEPIG QKNKKIHSKL HTSIKPKHSK KKWVFLRLLF ILFLSAGLVV
60 70 80 90 100
ILYGFYLNEK IRQRIDGQVW VLPAAVYGRM LNLEPGMAYS KKQMINLLIA
110 120 130 140 150
TQYRKVNHLL RVGEFTVQDN SIDMLRRPFN FPDAKEDQVH ARLLFHNDRL
160 170 180 190 200
VQIENLDNQR FFGFFRLDPR LITMLQTPSG EQRLFLPRSS FPESLVNTLL
210 220 230 240 250
AIEDRHFYQH DGINFYSIGR ALLANILAGR TVQGGSTLTQ QLVKNLFLNN
260 270 280 290 300
QRSFWRKANE AYMAILIDHH YSKDKILELY FNEVYLGQNG NDQIRGFPLA
310 320 330 340 350
SLYYFGRPVD ELSLDQQAML VGMVKGASLY NPWRNPEFTL ERRNLVLKLL
360 370 380 390 400
QNQGNIDEKL YHTLSTRPLD VQPKGGVIVP QPAFIEMVRQ ELKQKLGDKA
410 420 430 440 450
QHLSGAKIFT TMDPVSQDAA EKAIESGIPA LKGSEHDLSD LEGAMVVIDR
460 470 480 490 500
LSGEIRAIVG GSQPQFSGFN RALQARRSIG SLAKPAIYLS ALNQPHQYRF
510 520 530 540 550
NTWLFDQPLS LELPNGSVWE PKNYSRKFRG QVMLLDGLVD SLNVPTVNLG
560 570 580 590 600
MSIGLDQISQ TMKQLGAPAS TGPLMPSMLL GAINLTPLEV AQEYQTIASG
610 620 630 640 650
GNKATLSALR SVIAADGSML YQSFPQSERK LSAQASYLVL YGMQEAVKRG
660 670 680 690 700
TARSLSSEFS TFHLAGKTGT TNDLRDSWFA GIDGKEVTII WLGRDNNGPT
710 720 730 740 750
QFTGSTGALT LYRRYLETKP PLPLISRKPA GIVDMNIDST TGHFICDTEP
760 770 780 790
HNQISTIPVW TEDPDVLCKT SEKTTPPNEL KEKSVLDSIK NIF
Length:793
Mass (Da):89,165
Last modified:July 7, 2009 - v1
Checksum:iE2405417100E3143
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001277 Genomic DNA. Translation: ACQ67582.1.
RefSeqiYP_002923730.1. NC_012751.1.

Genome annotation databases

EnsemblBacteriaiACQ67582; ACQ67582; HDEF_0862.
KEGGihde:HDEF_0862.
PATRICi31973199. VBICanHam112931_0876.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001277 Genomic DNA. Translation: ACQ67582.1.
RefSeqiYP_002923730.1. NC_012751.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi572265.HDEF_0862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACQ67582; ACQ67582; HDEF_0862.
KEGGihde:HDEF_0862.
PATRICi31973199. VBICanHam112931_0876.

Phylogenomic databases

eggNOGiCOG0744.
KOiK05365.
OMAiIAQVWVT.
OrthoDBiEOG6HMXCD.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciHDEF572265:GJAB-868-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011813. PBP_1b.
IPR001460. PCN-bd_Tpept.
IPR028166. UB2H.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
PF14814. UB2H. 1 hit.
[Graphical view]
PIRSFiPIRSF002799. PBP_1b. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsiTIGR02071. PBP_1b. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Hamiltonella defensa, genome evolution of protective bacterial endosymbiont from pathogenic ancestors."
    Degnan P.H., Yu Y., Sisneros N., Wing R.A., Moran N.A.
    Proc. Natl. Acad. Sci. U.S.A. 106:9063-9068(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 5ATImported.

Entry informationi

Entry nameiC4K4T9_HAMD5
AccessioniPrimary (citable) accession number: C4K4T9
Entry historyi
Integrated into UniProtKB/TrEMBL: July 7, 2009
Last sequence update: July 7, 2009
Last modified: April 29, 2015
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.