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Protein

Glutamine--tRNA ligase

Gene

glnS

Organism
Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei271 – 2711ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutaminyl-tRNA aminoacylation Source: InterPro
  2. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHDEF572265:GJAB-769-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligaseUniRule annotation (EC:6.1.1.18UniRule annotation)
Alternative name(s):
Glutaminyl-tRNA synthetaseUniRule annotation
Short name:
GlnRSUniRule annotation
Gene namesi
Name:glnSUniRule annotation
Ordered Locus Names:HDEF_0764
OrganismiHamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT)
Taxonomic identifieri572265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeaphid secondary symbiontsCandidatus Hamiltonella
ProteomesiUP000002334: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 552552Glutamine--tRNA ligasePRO_1000203123Add
BLAST

Proteomic databases

PRIDEiC4K4J9.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi572265.HDEF_0764.

Structurei

3D structure databases

ProteinModelPortaliC4K4J9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi34 – 4411"HIGH" regionAdd
BLAST
Motifi268 – 2725"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
KOiK01886.
OMAiVTHSICT.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4K4J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEASERPNH FIRHIIEEDL AQGKHSFIRT RFPPEPNGYL HIGHAKSICL
60 70 80 90 100
NFGLAQDYQG ECHLRFDDTN PEKEEQEYIE SIKRDVQWLG FAWSGAVRYS
110 120 130 140 150
SDYFESLYGY AIELIQKELA YVDELNAEQM REYRGTLTAP GKNSPFRDRT
160 170 180 190 200
VEDNIELFKK MRAGGFAEGA ACLRAKIDMA SPFMIMRDPV LYRIKFAEHH
210 220 230 240 250
QLGDQWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL YDWVLENISI
260 270 280 290 300
DCHPRQYEFS RLNLEHTLMS KRKLHLLVKE KKVTGWDDPR MPTLSGLKRR
310 320 330 340 350
GYTPASIREF CHRIGVTKQD NQVEMSALEA CIREDLNQNA PRALAVLDPV
360 370 380 390 400
QLIIDNRSDE KEWLTVPNHP NNPGMGHRQM PFNRVLYIDR ADFREEANKK
410 420 430 440 450
YKRLVLGKEV RLRHAYVVKA KYVEKNAQGM ITAIHCEYDP ETLNKDPSDG
460 470 480 490 500
RKIRGVIHWV SADDAVPAEI RLYHHLLTAS HADTCLFLEA FNPESLFIRQ
510 520 530 540 550
GFVEPSLANT QEGKTYQFER EGYFCTDKDS SEKHLVFNRT VSLRGESALY

DL
Length:552
Mass (Da):64,151
Last modified:July 7, 2009 - v1
Checksum:i6E238F42C227F273
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001277 Genomic DNA. Translation: ACQ67492.1.
RefSeqiYP_002923640.1. NC_012751.1.

Genome annotation databases

EnsemblBacteriaiACQ67492; ACQ67492; HDEF_0764.
GeneIDi7950365.
KEGGihde:HDEF_0764.
PATRICi31972981. VBICanHam112931_0772.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001277 Genomic DNA. Translation: ACQ67492.1.
RefSeqiYP_002923640.1. NC_012751.1.

3D structure databases

ProteinModelPortaliC4K4J9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi572265.HDEF_0764.

Proteomic databases

PRIDEiC4K4J9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACQ67492; ACQ67492; HDEF_0764.
GeneIDi7950365.
KEGGihde:HDEF_0764.
PATRICi31972981. VBICanHam112931_0772.

Phylogenomic databases

eggNOGiCOG0008.
KOiK01886.
OMAiVTHSICT.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciHDEF572265:GJAB-769-MONOMER.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Hamiltonella defensa, genome evolution of protective bacterial endosymbiont from pathogenic ancestors."
    Degnan P.H., Yu Y., Sisneros N., Wing R.A., Moran N.A.
    Proc. Natl. Acad. Sci. U.S.A. 106:9063-9068(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 5AT.

Entry informationi

Entry nameiSYQ_HAMD5
AccessioniPrimary (citable) accession number: C4K4J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: January 7, 2015
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.