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C4K2T0

- SYI_RICPU

UniProt

C4K2T0 - SYI_RICPU

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Protein
Isoleucine--tRNA ligase
Gene
ileS, RPR_07180
Organism
Rickettsia peacockii (strain Rustic)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei616 – 6161ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRPEA562019:GJD7-901-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:RPR_07180
OrganismiRickettsia peacockii (strain Rustic)
Taxonomic identifieri562019 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000005015: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10921092Isoleucine--tRNA ligaseUniRule annotation
PRO_1000216257Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi562019.RPR_07180.

Structurei

3D structure databases

ProteinModelPortaliC4K2T0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionUniRule annotation
Add
BLAST
Motifi613 – 6175"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiAVETHRE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4K2T0-1 [UniParc]FASTAAdd to Basket

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MTNTKYYPEV SSNADFAGLE REILKFWQDN NIFQKSIDDR NGESEFIFYD     50
GPPFANGLPH YGHLLTGFIK DVYARYQTVK GKKVERRFGW DCHGLPAEMQ 100
SEKELGISGR LAIANFGIEK FNAHCRASVM KYANDWEEYV TRQARWVDFK 150
NSYKTMDKNF MESVLWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR 200
LDNSYRERAD KAVTVSFVLS HPVATTTGSF KEYRILAWTT TPWTLPSNLA 250
LAVGSDIDYA LVPKNDVCYI IAAYSVSKYA KELGLSGEEN FEIIKGSELQ 300
GLHYKSLFDY FENHPNSFKI FAGDFVVEGD GTGVVHMAPG FGEDDQILCE 350
SKGIELVCPV DNSGKFTKEI PNLEGLQVFD ANDKIIIKLK EQGNWLKTEQ 400
YIHNYPHCWR TDTPLIYKAV PSWYVKVTQF KDRMVELNQQ INWIPFHVKD 450
NLFGKWLENA RDWSISRNRF WGTPLPVWKS DDPKYPRIDV YGSIEELEKD 500
FGVKVTDLHR PFIDELTRPN PDDPTGKSTM RRIEDVFDCW FESGSMPYGQ 550
AHYPFENKEW FEDHFPADFI VEYSAQTRGW FYTLMVLSTA LFDRPPFLNC 600
ICHGVILDST GQKLSKRLNN YADPLELFDK YGSDALRVTM LSSNVVKGQE 650
LLIDKDGKMV FDTLRLFIKP IWNAYHFFTM YANADSLKGK LNFSSKNVLD 700
VYILSKLKIA VQKIEESLDN FDTQTAYHAV SEFFEVLNNW YIRRSRARFW 750
KSEKDTDKQN AYNTLYSCLD TMAIAMSALV PMISEAIYKG LRHCEERNDT 800
ALSGKSNVIA RQDTSLDKAI SGVSHKIATA LSVPRNDAIS VHLCNYPTLS 850
DFEINHELVA TMDNVLDICS NSLFIRSTEN IRVRQPLASI AIISKHNNNL 900
KDFEDLIKDE INVKAVIYRD DLENYTSKKL SINFPMLGKR LPHKMKEIIV 950
ASKKGEWEAI TGGLAICGET LNSDEYKLVL EPYSHIKGAA SFENNSSLLI 1000
LDLELTPELI EEGYARDIVR FIQQARKDAD FSITDRILIE IISEFNLSKI 1050
IDNYGDFIKE QTLGEFAKNF TPDYVSKVEL ENYQIQLKVK KS 1092
Length:1,092
Mass (Da):125,622
Last modified:July 7, 2009 - v1
Checksum:i3B4B4BD68D1C3416
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001227 Genomic DNA. Translation: ACR47875.1.
RefSeqiYP_002916929.1. NC_012730.1.

Genome annotation databases

EnsemblBacteriaiACR47875; ACR47875; RPR_07180.
GeneIDi7942092.
KEGGirpk:RPR_07180.
PATRICi17900450. VBIRicPea48268_1422.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001227 Genomic DNA. Translation: ACR47875.1 .
RefSeqi YP_002916929.1. NC_012730.1.

3D structure databases

ProteinModelPortali C4K2T0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 562019.RPR_07180.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR47875 ; ACR47875 ; RPR_07180 .
GeneIDi 7942092.
KEGGi rpk:RPR_07180.
PATRICi 17900450. VBIRicPea48268_1422.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi AVETHRE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RPEA562019:GJD7-901-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endosymbiont Rickettsia peacockii and comparison with virulent Rickettsia rickettsii: identification of virulence factors."
    Felsheim R.F., Kurtti T.J., Munderloh U.G.
    PLoS ONE 4:E8361-E8361(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rustic.

Entry informationi

Entry nameiSYI_RICPU
AccessioniPrimary (citable) accession number: C4K2T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi