Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

C4K2T0

- SYI_RICPU

UniProt

C4K2T0 - SYI_RICPU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia peacockii (strain Rustic)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (07 Jul 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei616 – 6161ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciRPEA562019:GJD7-901-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:RPR_07180
    OrganismiRickettsia peacockii (strain Rustic)
    Taxonomic identifieri562019 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000005015: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10921092Isoleucine--tRNA ligasePRO_1000216257Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi562019.RPR_07180.

    Structurei

    3D structure databases

    ProteinModelPortaliC4K2T0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi53 – 6311"HIGH" regionAdd
    BLAST
    Motifi613 – 6175"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiAVETHRE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 2 hits.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C4K2T0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNTKYYPEV SSNADFAGLE REILKFWQDN NIFQKSIDDR NGESEFIFYD     50
    GPPFANGLPH YGHLLTGFIK DVYARYQTVK GKKVERRFGW DCHGLPAEMQ 100
    SEKELGISGR LAIANFGIEK FNAHCRASVM KYANDWEEYV TRQARWVDFK 150
    NSYKTMDKNF MESVLWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR 200
    LDNSYRERAD KAVTVSFVLS HPVATTTGSF KEYRILAWTT TPWTLPSNLA 250
    LAVGSDIDYA LVPKNDVCYI IAAYSVSKYA KELGLSGEEN FEIIKGSELQ 300
    GLHYKSLFDY FENHPNSFKI FAGDFVVEGD GTGVVHMAPG FGEDDQILCE 350
    SKGIELVCPV DNSGKFTKEI PNLEGLQVFD ANDKIIIKLK EQGNWLKTEQ 400
    YIHNYPHCWR TDTPLIYKAV PSWYVKVTQF KDRMVELNQQ INWIPFHVKD 450
    NLFGKWLENA RDWSISRNRF WGTPLPVWKS DDPKYPRIDV YGSIEELEKD 500
    FGVKVTDLHR PFIDELTRPN PDDPTGKSTM RRIEDVFDCW FESGSMPYGQ 550
    AHYPFENKEW FEDHFPADFI VEYSAQTRGW FYTLMVLSTA LFDRPPFLNC 600
    ICHGVILDST GQKLSKRLNN YADPLELFDK YGSDALRVTM LSSNVVKGQE 650
    LLIDKDGKMV FDTLRLFIKP IWNAYHFFTM YANADSLKGK LNFSSKNVLD 700
    VYILSKLKIA VQKIEESLDN FDTQTAYHAV SEFFEVLNNW YIRRSRARFW 750
    KSEKDTDKQN AYNTLYSCLD TMAIAMSALV PMISEAIYKG LRHCEERNDT 800
    ALSGKSNVIA RQDTSLDKAI SGVSHKIATA LSVPRNDAIS VHLCNYPTLS 850
    DFEINHELVA TMDNVLDICS NSLFIRSTEN IRVRQPLASI AIISKHNNNL 900
    KDFEDLIKDE INVKAVIYRD DLENYTSKKL SINFPMLGKR LPHKMKEIIV 950
    ASKKGEWEAI TGGLAICGET LNSDEYKLVL EPYSHIKGAA SFENNSSLLI 1000
    LDLELTPELI EEGYARDIVR FIQQARKDAD FSITDRILIE IISEFNLSKI 1050
    IDNYGDFIKE QTLGEFAKNF TPDYVSKVEL ENYQIQLKVK KS 1092
    Length:1,092
    Mass (Da):125,622
    Last modified:July 7, 2009 - v1
    Checksum:i3B4B4BD68D1C3416
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001227 Genomic DNA. Translation: ACR47875.1.
    RefSeqiYP_002916929.1. NC_012730.1.

    Genome annotation databases

    EnsemblBacteriaiACR47875; ACR47875; RPR_07180.
    GeneIDi7942092.
    KEGGirpk:RPR_07180.
    PATRICi17900450. VBIRicPea48268_1422.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001227 Genomic DNA. Translation: ACR47875.1 .
    RefSeqi YP_002916929.1. NC_012730.1.

    3D structure databases

    ProteinModelPortali C4K2T0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 562019.RPR_07180.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACR47875 ; ACR47875 ; RPR_07180 .
    GeneIDi 7942092.
    KEGGi rpk:RPR_07180.
    PATRICi 17900450. VBIRicPea48268_1422.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi AVETHRE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci RPEA562019:GJD7-901-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 2 hits.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endosymbiont Rickettsia peacockii and comparison with virulent Rickettsia rickettsii: identification of virulence factors."
      Felsheim R.F., Kurtti T.J., Munderloh U.G.
      PLoS ONE 4:E8361-E8361(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Rustic.

    Entry informationi

    Entry nameiSYI_RICPU
    AccessioniPrimary (citable) accession number: C4K2T0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3