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C4K2T0

- SYI_RICPU

UniProt

C4K2T0 - SYI_RICPU

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia peacockii (strain Rustic)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei616 – 6161ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRPEA562019:GJD7-901-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:RPR_07180
OrganismiRickettsia peacockii (strain Rustic)
Taxonomic identifieri562019 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000005015: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10921092Isoleucine--tRNA ligasePRO_1000216257Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi562019.RPR_07180.

Structurei

3D structure databases

ProteinModelPortaliC4K2T0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionAdd
BLAST
Motifi613 – 6175"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiAVETHRE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4K2T0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNTKYYPEV SSNADFAGLE REILKFWQDN NIFQKSIDDR NGESEFIFYD
60 70 80 90 100
GPPFANGLPH YGHLLTGFIK DVYARYQTVK GKKVERRFGW DCHGLPAEMQ
110 120 130 140 150
SEKELGISGR LAIANFGIEK FNAHCRASVM KYANDWEEYV TRQARWVDFK
160 170 180 190 200
NSYKTMDKNF MESVLWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR
210 220 230 240 250
LDNSYRERAD KAVTVSFVLS HPVATTTGSF KEYRILAWTT TPWTLPSNLA
260 270 280 290 300
LAVGSDIDYA LVPKNDVCYI IAAYSVSKYA KELGLSGEEN FEIIKGSELQ
310 320 330 340 350
GLHYKSLFDY FENHPNSFKI FAGDFVVEGD GTGVVHMAPG FGEDDQILCE
360 370 380 390 400
SKGIELVCPV DNSGKFTKEI PNLEGLQVFD ANDKIIIKLK EQGNWLKTEQ
410 420 430 440 450
YIHNYPHCWR TDTPLIYKAV PSWYVKVTQF KDRMVELNQQ INWIPFHVKD
460 470 480 490 500
NLFGKWLENA RDWSISRNRF WGTPLPVWKS DDPKYPRIDV YGSIEELEKD
510 520 530 540 550
FGVKVTDLHR PFIDELTRPN PDDPTGKSTM RRIEDVFDCW FESGSMPYGQ
560 570 580 590 600
AHYPFENKEW FEDHFPADFI VEYSAQTRGW FYTLMVLSTA LFDRPPFLNC
610 620 630 640 650
ICHGVILDST GQKLSKRLNN YADPLELFDK YGSDALRVTM LSSNVVKGQE
660 670 680 690 700
LLIDKDGKMV FDTLRLFIKP IWNAYHFFTM YANADSLKGK LNFSSKNVLD
710 720 730 740 750
VYILSKLKIA VQKIEESLDN FDTQTAYHAV SEFFEVLNNW YIRRSRARFW
760 770 780 790 800
KSEKDTDKQN AYNTLYSCLD TMAIAMSALV PMISEAIYKG LRHCEERNDT
810 820 830 840 850
ALSGKSNVIA RQDTSLDKAI SGVSHKIATA LSVPRNDAIS VHLCNYPTLS
860 870 880 890 900
DFEINHELVA TMDNVLDICS NSLFIRSTEN IRVRQPLASI AIISKHNNNL
910 920 930 940 950
KDFEDLIKDE INVKAVIYRD DLENYTSKKL SINFPMLGKR LPHKMKEIIV
960 970 980 990 1000
ASKKGEWEAI TGGLAICGET LNSDEYKLVL EPYSHIKGAA SFENNSSLLI
1010 1020 1030 1040 1050
LDLELTPELI EEGYARDIVR FIQQARKDAD FSITDRILIE IISEFNLSKI
1060 1070 1080 1090
IDNYGDFIKE QTLGEFAKNF TPDYVSKVEL ENYQIQLKVK KS
Length:1,092
Mass (Da):125,622
Last modified:July 7, 2009 - v1
Checksum:i3B4B4BD68D1C3416
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001227 Genomic DNA. Translation: ACR47875.1.
RefSeqiYP_002916929.1. NC_012730.1.

Genome annotation databases

EnsemblBacteriaiACR47875; ACR47875; RPR_07180.
GeneIDi7942092.
KEGGirpk:RPR_07180.
PATRICi17900450. VBIRicPea48268_1422.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001227 Genomic DNA. Translation: ACR47875.1 .
RefSeqi YP_002916929.1. NC_012730.1.

3D structure databases

ProteinModelPortali C4K2T0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 562019.RPR_07180.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR47875 ; ACR47875 ; RPR_07180 .
GeneIDi 7942092.
KEGGi rpk:RPR_07180.
PATRICi 17900450. VBIRicPea48268_1422.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi AVETHRE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RPEA562019:GJD7-901-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endosymbiont Rickettsia peacockii and comparison with virulent Rickettsia rickettsii: identification of virulence factors."
    Felsheim R.F., Kurtti T.J., Munderloh U.G.
    PLoS ONE 4:E8361-E8361(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rustic.

Entry informationi

Entry nameiSYI_RICPU
AccessioniPrimary (citable) accession number: C4K2T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: October 29, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3