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C4K297 (SYL_RICPU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:RPR_05595
OrganismRickettsia peacockii (strain Rustic) [Complete proteome] [HAMAP]
Taxonomic identifier562019 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 835835Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000202228

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00049
Motif602 – 6065"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6051ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C4K297 [UniParc].

Last modified July 7, 2009. Version 1.
Checksum: 57C124CBC3C0597E

FASTA83596,422
        10         20         30         40         50         60 
MNQIEQKWQY IWQEEKAFEV SNASSKPKYY VLEMLPYPSG KIHVGHVRNY SIGDVIARFM 

        70         80         90        100        110        120 
TMQGFNVLHP MGWDAFGLPA ENAAIKNNSH PKKWTYSNIK NMKKQLKSMG FSYDWSREIN 

       130        140        150        160        170        180 
SCDPEYYKHE QKFFLELYER NLAYQKASFV NWDPVDNTVL ANEQVVDGRG WRSGAIVAKR 

       190        200        210        220        230        240 
YLKQWFLKIT DYAEELLNEI QNLKEWPEAV RSMQEKWIGK SIGANFHFKI KDNEETTIEV 

       250        260        270        280        290        300 
FSTKPETIFG ASFIGIAFNH PIIERLVSKT PEILAFITQC SHITRSSELE KAEKEGVFTG 

       310        320        330        340        350        360 
LFVTHPFDSN IVLPVIITNF VLMDYGTGAI FGCPAHDECD HELAVKMNLS IKQVIKADMD 

       370        380        390        400        410        420 
VQKTAYTEDG ILINSDVLNG LTSNEAKQEV IGEFEKLGIG KRSVNYRLKD WGISRQRFWG 

       430        440        450        460        470        480 
CPIPMIHCEI CGIVPVPYKD LPVTLPDDVN FDGHGNPLDH HPSWKHVNCP KCDKPAVRET 

       490        500        510        520        530        540 
DTFDTFFESS WYFTRYCNSN ATEMTDKKAC DYWLPVDKYI GGIEHAVMHL LYARFFTKVM 

       550        560        570        580        590        600 
NEQNYVSVRE PFKGLFTQGM VLHATYKDEH NNWLYPEEVV KKGNEFFHKE SNNRVVQGRI 

       610        620        630        640        650        660 
EKMSKSKKNL IDLETMQEQY GADAIRLFVL SDSPPEKDLE WSASGIEGCS RFINKLEYMF 

       670        680        690        700        710        720 
KAIDSLKDDV NSEVNKELNR LVHFTIKHVA EDIKHFALNR AIARMRELSN AISAEISKDK 

       730        740        750        760        770        780 
IDVKIVRHGF NVLVQLLNPF IPHITEEIWQ KLGNKERLYN LSFPAFDESM LELDTYIMAV 

       790        800        810        820        830 
QVNGKLRDTY EFKTSVSEDE IKQVTVSLPK VQKCLEGKEP KKIILVPRKI VNILV 

« Hide

References

[1]"Genome sequence of the endosymbiont Rickettsia peacockii and comparison with virulent Rickettsia rickettsii: identification of virulence factors."
Felsheim R.F., Kurtti T.J., Munderloh U.G.
PLoS ONE 4:E8361-E8361(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rustic.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001227 Genomic DNA. Translation: ACR47694.1.
RefSeqYP_002916746.1. NC_012730.1.

3D structure databases

ProteinModelPortalC4K297.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING562019.RPR_05595.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACR47694; ACR47694; RPR_05595.
GeneID7942230.
KEGGrpk:RPR_05595.
PATRIC17899800. VBIRicPea48268_1107.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycRPEA562019:GJD7-709-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RICPU
AccessionPrimary (citable) accession number: C4K297
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries