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C4JE77

- LIPA_UNCRE

UniProt

C4JE77 - LIPA_UNCRE

Protein

Lipoyl synthase, mitochondrial

Gene

UREG_00501

Organism
Uncinocarpus reesii (strain UAMH 1704)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (07 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi134 – 1341Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi139 – 1391Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi145 – 1451Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi165 – 1651Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi169 – 1691Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi172 – 1721Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    ORF Names:UREG_00501
    OrganismiUncinocarpus reesii (strain UAMH 1704)
    Taxonomic identifieri336963 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesOnygenaceaeUncinocarpus
    ProteomesiUP000002058: Unassembled WGS sequence

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2626MitochondrionUniRule annotationAdd
    BLAST
    Chaini27 – 417391Lipoyl synthase, mitochondrialPRO_0000398295Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliC4JE77.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK03644.
    OrthoDBiEOG79KPR7.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C4JE77-1 [UniParc]FASTAAdd to Basket

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    MAVCARGLRC LGTPAVSLRL AASRSYATTT PPDPAIPNTP GAAATSSPAK    50
    RPRTSFQDKL NAGPSFSDFL SDKDDARILD PAEAYALKTA LVGPKGKKKE 100
    YTRLPPWLKT SIPDSNNYKR IKNDLRGLNL HTVCEEARCP NISECWGGGS 150
    KSAATATIML MGDTCTRGCR FCSVKTSKAP PPLDPHEPEN TAEALSRWGL 200
    GYVVMTSVDR DDLPDGGARH WAETVMKIKQ KAPNILVECL TGDFDGNLEM 250
    VALVAKSGLD VYAHNVETVE ALTPQVRDRR AGFQKSIRVL KAAKAAQPSL 300
    ITKTSMMLGL GETEEQMWDA LRQLRAADID VVTFGQYMRP TKRHMPVHEY 350
    VRPDVFEFWK EKALEMGFLY CASGPLVRSS YKAGEAFIEN VLKKRRAEST 400
    GPGSASVQDV ATGDLVR 417
    Length:417
    Mass (Da):45,521
    Last modified:July 7, 2009 - v1
    Checksum:i69CA8F411C8B3B91
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH476615 Genomic DNA. Translation: EEP75655.1.
    RefSeqiXP_002540988.1. XM_002540942.1.

    Genome annotation databases

    GeneIDi8437299.
    KEGGiure:UREG_00501.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH476615 Genomic DNA. Translation: EEP75655.1 .
    RefSeqi XP_002540988.1. XM_002540942.1.

    3D structure databases

    ProteinModelPortali C4JE77.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 8437299.
    KEGGi ure:UREG_00501.

    Phylogenomic databases

    KOi K03644.
    OrthoDBi EOG79KPR7.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: UAMH 1704.

    Entry informationi

    Entry nameiLIPA_UNCRE
    AccessioniPrimary (citable) accession number: C4JE77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3