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C4JE77

- LIPA_UNCRE

UniProt

C4JE77 - LIPA_UNCRE

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Protein

Lipoyl synthase, mitochondrial

Gene

UREG_00501

Organism
Uncinocarpus reesii (strain UAMH 1704)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi134 – 1341Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi139 – 1391Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi145 – 1451Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi165 – 1651Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi169 – 1691Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi172 – 1721Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:UREG_00501
OrganismiUncinocarpus reesii (strain UAMH 1704)
Taxonomic identifieri336963 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesOnygenaceaeUncinocarpus
ProteomesiUP000002058: Unassembled WGS sequence

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626MitochondrionUniRule annotationAdd
BLAST
Chaini27 – 417391Lipoyl synthase, mitochondrialPRO_0000398295Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliC4JE77.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiC4JE77.
KOiK03644.
OrthoDBiEOG79KPR7.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C4JE77-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVCARGLRC LGTPAVSLRL AASRSYATTT PPDPAIPNTP GAAATSSPAK
60 70 80 90 100
RPRTSFQDKL NAGPSFSDFL SDKDDARILD PAEAYALKTA LVGPKGKKKE
110 120 130 140 150
YTRLPPWLKT SIPDSNNYKR IKNDLRGLNL HTVCEEARCP NISECWGGGS
160 170 180 190 200
KSAATATIML MGDTCTRGCR FCSVKTSKAP PPLDPHEPEN TAEALSRWGL
210 220 230 240 250
GYVVMTSVDR DDLPDGGARH WAETVMKIKQ KAPNILVECL TGDFDGNLEM
260 270 280 290 300
VALVAKSGLD VYAHNVETVE ALTPQVRDRR AGFQKSIRVL KAAKAAQPSL
310 320 330 340 350
ITKTSMMLGL GETEEQMWDA LRQLRAADID VVTFGQYMRP TKRHMPVHEY
360 370 380 390 400
VRPDVFEFWK EKALEMGFLY CASGPLVRSS YKAGEAFIEN VLKKRRAEST
410
GPGSASVQDV ATGDLVR
Length:417
Mass (Da):45,521
Last modified:July 7, 2009 - v1
Checksum:i69CA8F411C8B3B91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476615 Genomic DNA. Translation: EEP75655.1.
RefSeqiXP_002540988.1. XM_002540942.1.

Genome annotation databases

GeneIDi8437299.
KEGGiure:UREG_00501.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476615 Genomic DNA. Translation: EEP75655.1 .
RefSeqi XP_002540988.1. XM_002540942.1.

3D structure databases

ProteinModelPortali C4JE77.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8437299.
KEGGi ure:UREG_00501.

Phylogenomic databases

InParanoidi C4JE77.
KOi K03644.
OrthoDBi EOG79KPR7.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UAMH 1704.

Entry informationi

Entry nameiLIPA_UNCRE
AccessioniPrimary (citable) accession number: C4JE77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 7, 2009
Last modified: November 26, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3