C4AMC7 (WASH3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative WAS protein family homolog 3

Alternative name(s):
Protein FAM39DP

Gene names

Name:	WASH3P
Synonyms:	FAM39DP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting By similarity.
Subunit structure	Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Interacts (via WHD1 region) with FAM21C; the interaction is direct By similarity.
Subcellular location	Early endosome membrane By similarity. Recycling endosome membrane By similarity. Note: Localization to the endodome membrane is mediated via its interaction with FAM21 By similarity.
Miscellaneous	WASH genes duplicated to multiple chromosomal ends during primate evolution, with highest copy number reached in humans, whose WASH repertoires probably vary extensively among individuals (Ref.4). It is therefore difficult to determine which gene is functional or not. The telomeric region of chromosome 9p is paralogous to the pericentromeric regions of chromosome 9 as well as to 2q. Paralogous regions contain 7 transcriptional units. Duplicated WASH genes are also present in the Xq/Yq pseudoautosomal region, as well as on chromosome 1 and 15. The chromosome 16 copy seems to be a pseudogene.
Sequence similarities	Belongs to the WASH1 family. Contains 1 WH2 domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Endosome Membrane
Ligand	Actin-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Arp2/3 complex-mediated actin nucleation Inferred from direct assay Ref.4. Source: UniProtKB retrograde transport, endosome to Golgi Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	WASH complex Inferred from sequence or structural similarity. Source: UniProtKB early endosome Inferred from sequence or structural similarity. Source: UniProtKB early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell filopodium Inferred from direct assay Ref.4. Source: UniProtKB lamellipodium Inferred from direct assay Ref.4. Source: UniProtKB recycling endosome Inferred from sequence or structural similarity. Source: UniProtKB recycling endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Putative WAS protein family homolog 3		PRO_0000390962
Regions
	Domain	359 – 381	23	WH2
	Region	1 – 165	165	WHD1
	Compositional bias	266 – 328	63	Pro-rich
	Compositional bias	338 – 345	8	Poly-Ser
Experimental info
	Sequence conflict	54	1	Q → R in BC048328. Ref.2
	Sequence conflict	119	1	P → S in BC048328. Ref.2
	Sequence conflict	139	1	D → LKY in BC048328. Ref.2
	Sequence conflict	181	1	G → Y in BC048328. Ref.2
	Sequence conflict	255	1	T → A in BC048328. Ref.2
	Sequence conflict	261	1	I → S in BC048328. Ref.2
	Sequence conflict	292	1	V → A in BC048328. Ref.2
	Sequence conflict	300	1	T → TAPP in BC048328. Ref.2
	Sequence conflict	319	1	P → S in BC048328. Ref.2
	Sequence conflict	381	1	M → V in BC048328. Ref.2
	Sequence conflict	390	1	Q → K in BC048328. Ref.2
	Sequence conflict	405	1	H → D in BC048328. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	C4AMC7 [UniParc]. Last modified November 3, 2009. Version 2. Checksum: FD6B57565AFDAB82	FASTA	463	49,995
	10 20 30 40 50 60 MTPVRMQHSL AGQTYAVPLI QPDLRREEAV QQMADALQYL QKVSGDIFSR ISQQVEQSRS 70 80 90 100 110 120 QVQAIGEKVS LAQAKIEKIK GSKKAIKVFS SAKYPAPERL QEYGSIFTGA QDPGLQRRPR 130 140 150 160 170 180 HRIQSKHRPL DERALQEKDF PVCVSTKPEP EDDAEEGLGG LPSNISSVSS LLLFNTTENL 190 200 210 220 230 240 GKKYVFLDPL AGAVTKTHVM LGAETEEKLF DAPLSISKRE QLEQQVPENY FYVPDLGQVP 250 260 270 280 290 300 EIDVPSYLPD LPGITNDLMY IADLGPGIAP SAPGTIPELP TFHTEVAEPL KVDLQDGVLT 310 320 330 340 350 360 PPPPPPPPPP APEVLASAPP LPPSTAAPVG QGARQDDSSS SASPSVQGAP REVVDPSGGR 370 380 390 400 410 420 ATLLESIRQA GGIGKAKLRS MKERKLEKKQ QKEQEQVRAT SQGGHLMSDL FNKLVMRRKG 430 440 450 460 ISGKGPGAGE GPGGAFARVS DSIPPLPPPQ QPQAEEDEDD WES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Differentially regulated and evolved genes in the fully sequenced Xq/Yq pseudoautosomal region." Ciccodicola A., D'Esposito M., Esposito T., Gianfrancesco F., Migliaccio C., Miano M.G., Matarazzo M.R., Vacca M., Franze A., Cuccurese M., Cocchia M., Curci A., Terracciano A., Torino A., Cocchia S., Mercadante G., Pannone E., Archidiacono N. , Rocchi M., Schlessinger D., D'Urso M. Hum. Mol. Genet. 9:395-401(2000) [PubMed] [Europe PMC] [Abstract] Cited for: GENE DUPLICATION.
[4]	"Human subtelomeric WASH genes encode a new subclass of the WASP family." Linardopoulou E.V., Parghi S.S., Friedman C., Osborn G.E., Parkhurst S.M., Trask B.J. PLoS Genet. 3:E237-E237(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE DUPLICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC140725 Genomic DNA. No translation available. BC048328 mRNA. No translation available.
IPI	IPI00815683.
UniGene	Hs.459573. Hs.585931.
3D structure databases
ProteinModelPortal	C4AMC7.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000405426.
Proteomic databases
PaxDb	C4AMC7.
PRIDE	C4AMC7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	uc002cdi.3. human.
Organism-specific databases
GeneCards	GC15P102501.
HGNC	HGNC:24362. WASH3P.
HPA	HPA002689.
neXtProt	NX_C4AMC7.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG86663.
Family and domain databases
InterPro	IPR021854. WASH_WASD. IPR003124. WH2_dom. [Graphical view]
Pfam	PF11945. WASH_WAHD. 1 hit. [Graphical view]
PROSITE	PS51082. WH2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WASH3_HUMAN

Accession

Primary (citable) accession number: C4AMC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 19, 2010
Last sequence update:	November 3, 2009
Last modified:	April 3, 2013
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.