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C3Y3G4

- LIAS_BRAFL

UniProt

C3Y3G4 - LIAS_BRAFL

Protein

Lipoyl synthase, mitochondrial

Gene

BRAFLDRAFT_125969

Organism
Branchiostoma floridae (Florida lancelet) (Amphioxus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi102 – 1021Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi107 – 1071Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi113 – 1131Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi133 – 1331Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi140 – 1401Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    ORF Names:BRAFLDRAFT_125969
    OrganismiBranchiostoma floridae (Florida lancelet) (Amphioxus)
    Taxonomic identifieri7739 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCephalochordataBranchiostomidaeBranchiostoma
    ProteomesiUP000001554: Partially assembled WGS sequence

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 376Lipoyl synthase, mitochondrialPRO_0000398213
    Transit peptidei1 – ?MitochondrionUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi7739.JGI125969.

    Structurei

    3D structure databases

    ProteinModelPortaliC3Y3G4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    KOiK03644.
    OMAiPNIAECF.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C3Y3G4-1 [UniParc]FASTAAdd to Basket

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    MATCLLMSTF TFIRCSVLKW GSGSSLPQEQ KEKITKGPGL GDFISGEVES    50
    TDSWEDYMGK LRLEKGDKRL RLPPWLKKEI PIGKNYHSLK GTLRELNLAT 100
    VCEEAKCPNI GECWGGGEDK TATATIMVMG DTCTRGCRFC SVKTARSPPP 150
    LDPNEPVHTA EAISRWGVDY IVITSVDRDD VADGGSAHFS ECVKEIKKRI 200
    PSMLVECLTP DFRGDMEAVA TVAQSGLDVF AHNIETVKRL TPFVRDPRAK 250
    YDQSLQVLSH VKKTVPDMVT KSSIMLGLGE TDQEVRTAME DLRRAGVDCL 300
    TLGQYMQPTK RHLKVQEYVT PTKFKHWEEV GGEMGFAYTA SGPLVRSSYR 350
    AGEFYIKNLL NKKRTEATLD TSSQES 376
    Length:376
    Mass (Da):41,818
    Last modified:July 28, 2009 - v1
    Checksum:i9B44DD05AF6265F9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG666483 Genomic DNA. Translation: EEN65231.1.
    RefSeqiXP_002609221.1. XM_002609175.1.
    UniGeneiBfl.339.

    Genome annotation databases

    GeneIDi7219245.
    KEGGibfo:BRAFLDRAFT_125969.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG666483 Genomic DNA. Translation: EEN65231.1 .
    RefSeqi XP_002609221.1. XM_002609175.1.
    UniGenei Bfl.339.

    3D structure databases

    ProteinModelPortali C3Y3G4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7739.JGI125969.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 7219245.
    KEGGi bfo:BRAFLDRAFT_125969.

    Phylogenomic databases

    eggNOGi COG0320.
    KOi K03644.
    OMAi PNIAECF.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: S238N-H82.
      Tissue: Testis.

    Entry informationi

    Entry nameiLIAS_BRAFL
    AccessioniPrimary (citable) accession number: C3Y3G4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3