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C3Y3G4

- LIAS_BRAFL

UniProt

C3Y3G4 - LIAS_BRAFL

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Protein

Lipoyl synthase, mitochondrial

Gene
BRAFLDRAFT_125969
Organism
Branchiostoma floridae (Florida lancelet) (Amphioxus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi102 – 1021Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi107 – 1071Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi113 – 1131Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi133 – 1331Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi140 – 1401Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
ORF Names:BRAFLDRAFT_125969
OrganismiBranchiostoma floridae (Florida lancelet) (Amphioxus)
Taxonomic identifieri7739 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCephalochordataBranchiostomidaeBranchiostoma
ProteomesiUP000001554: Partially assembled WGS sequence

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 376Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398213
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Interactioni

Protein-protein interaction databases

STRINGi7739.JGI125969.

Structurei

3D structure databases

ProteinModelPortaliC3Y3G4.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.
OMAiPNIAECF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C3Y3G4-1 [UniParc]FASTAAdd to Basket

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MATCLLMSTF TFIRCSVLKW GSGSSLPQEQ KEKITKGPGL GDFISGEVES    50
TDSWEDYMGK LRLEKGDKRL RLPPWLKKEI PIGKNYHSLK GTLRELNLAT 100
VCEEAKCPNI GECWGGGEDK TATATIMVMG DTCTRGCRFC SVKTARSPPP 150
LDPNEPVHTA EAISRWGVDY IVITSVDRDD VADGGSAHFS ECVKEIKKRI 200
PSMLVECLTP DFRGDMEAVA TVAQSGLDVF AHNIETVKRL TPFVRDPRAK 250
YDQSLQVLSH VKKTVPDMVT KSSIMLGLGE TDQEVRTAME DLRRAGVDCL 300
TLGQYMQPTK RHLKVQEYVT PTKFKHWEEV GGEMGFAYTA SGPLVRSSYR 350
AGEFYIKNLL NKKRTEATLD TSSQES 376
Length:376
Mass (Da):41,818
Last modified:July 28, 2009 - v1
Checksum:i9B44DD05AF6265F9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GG666483 Genomic DNA. Translation: EEN65231.1.
RefSeqiXP_002609221.1. XM_002609175.1.
UniGeneiBfl.339.

Genome annotation databases

GeneIDi7219245.
KEGGibfo:BRAFLDRAFT_125969.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GG666483 Genomic DNA. Translation: EEN65231.1 .
RefSeqi XP_002609221.1. XM_002609175.1.
UniGenei Bfl.339.

3D structure databases

ProteinModelPortali C3Y3G4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7739.JGI125969.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 7219245.
KEGGi bfo:BRAFLDRAFT_125969.

Phylogenomic databases

eggNOGi COG0320.
KOi K03644.
OMAi PNIAECF.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S238N-H82.
    Tissue: Testis.

Entry informationi

Entry nameiLIAS_BRAFL
AccessioniPrimary (citable) accession number: C3Y3G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 28, 2009
Last modified: June 11, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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