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Protein

Neuraminidase

Gene

NA

Organism
Influenza A virus (strain swl A/California/04/2009 H1N1)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.UniRule annotationSAAS annotation

Cofactori

Ca2+UniRule annotationSAAS annotation

Enzyme regulationi

Inhibited by the neuraminidase inhibitors zanamivir (Relenza) and oseltamivir (Tamiflu). These drugs interfere with the release of progeny virus from infected cells and are effective against all influenza strains. Resistance to neuraminidase inhibitors is quite rare.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118SubstrateUniRule annotation1
Active sitei151Proton donor/acceptorUniRule annotation1
Binding sitei152SubstrateUniRule annotation1
Binding sitei293SubstrateUniRule annotation1
Metal bindingi294Calcium 1; via carbonyl oxygenCombined sources1
Metal bindingi294Calcium; via carbonyl oxygenUniRule annotation1
Metal bindingi298Calcium 1; via carbonyl oxygenCombined sources1
Metal bindingi298Calcium; via carbonyl oxygenUniRule annotation1
Metal bindingi324CalciumUniRule annotation1
Metal bindingi324Calcium 1Combined sources1
Binding sitei327MannoseCombined sources1
Metal bindingi342Calcium 1; via carbonyl oxygenCombined sources1
Metal bindingi344Calcium 1; via carbonyl oxygenCombined sources1
Metal bindingi344Calcium; via carbonyl oxygenUniRule annotation1
Binding sitei368SubstrateUniRule annotation1
Binding sitei372MannoseCombined sources1
Metal bindingi376Calcium 2Combined sources1
Metal bindingi378Calcium 2Combined sources1
Metal bindingi384Calcium 2Combined sources1
Metal bindingi386Calcium 2; via carbonyl oxygenCombined sources1
Active sitei402NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotationSAAS annotation, Hydrolase
LigandCalciumUniRule annotationCombined sourcesSAAS annotation, Metal-bindingUniRule annotationCombined sourcesSAAS annotation

Protein family/group databases

CAZyiGH34 Glycoside Hydrolase Family 34

Names & Taxonomyi

Protein namesi
Recommended name:
NeuraminidaseUniRule annotationSAAS annotation (EC:3.2.1.18UniRule annotationSAAS annotation)
Gene namesi
Name:NAUniRule annotationImported
OrganismiInfluenza A virus (strain swl A/California/04/2009 H1N1)Imported
Taxonomic identifieri641501 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeAlphainfluenzavirus
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000132424 Componenti: Genome

Subcellular locationi

  • Host apical cell membrane SAAS annotation; Single-pass type II membrane protein SAAS annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 34HelicalUniRule annotationAdd BLAST28

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membraneUniRule annotationSAAS annotation, Host membrane, Membrane, VirionUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi88N-linked (GlcNAc...)Combined sources1
Disulfide bondi92 ↔ 417UniRule annotationCombined sources
Disulfide bondi124 ↔ 129UniRule annotationCombined sources
Glycosylationi146N-linked (GlcNAc...)Combined sources1
Disulfide bondi184 ↔ 231UniRule annotationCombined sources
Disulfide bondi233 ↔ 238UniRule annotationCombined sources
Glycosylationi235N-linked (GlcNAc...)Combined sources1
Disulfide bondi279 ↔ 292UniRule annotationCombined sources
Disulfide bondi281 ↔ 290UniRule annotationCombined sources
Disulfide bondi318 ↔ 335UniRule annotationCombined sources
Disulfide bondi421 ↔ 446UniRule annotationCombined sources

Post-translational modificationi

N-glycosylated.UniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotationSAAS annotation, GlycoproteinUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-15878848,EBI-15878848

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-59036N

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NSSX-ray1.90A/B82-469[»]
3TI3X-ray1.80A/B82-469[»]
3TI4X-ray1.60A/B82-469[»]
3TI5X-ray1.90A/B82-469[»]
3TI6X-ray1.69A/B82-469[»]
SMRiC3W5S3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 33Involved in apical transport and lipid raft associationUniRule annotationAdd BLAST23
Regioni91 – 469Head of neuraminidaseUniRule annotationAdd BLAST379
Regioni277 – 278Substrate bindingUniRule annotation2
Regioni361 – 363Mannose bindingCombined sources3

Domaini

Intact N-terminus is essential for virion morphogenesis. Possess two apical sorting signals, one in the ectodomain, which is likely to be a glycan, and the other in the transmembrane domain. The transmembrane domain also plays a role in lipid raft association.UniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 34 family.UniRule annotationSAAS annotation

Keywords - Domaini

Signal-anchorUniRule annotation, Transmembrane, Transmembrane helixUniRule annotationSAAS annotation

Family and domain databases

CDDicd15483 Influenza_NA, 1 hit
HAMAPiMF_04071 INFV_NRAM, 1 hit
InterProiView protein in InterPro
IPR001860 Glyco_hydro_34
IPR033654 Sialidase_Influenza_A/B
IPR036278 Sialidase_sf
PfamiView protein in Pfam
PF00064 Neur, 1 hit
SUPFAMiSSF50939 SSF50939, 1 hit

Sequencei

Sequence statusi: Complete.

C3W5S3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPNQKIITI GSVCMTIGMA NLILQIGNII SIWISHSIQL GNQNQIETCN
60 70 80 90 100
QSVITYENNT WVNQTYVNIS NTNFAAGQSV VSVKLAGNSS LCPVSGWAIY
110 120 130 140 150
SKDNSVRIGS KGDVFVIREP FISCSPLECR TFFLTQGALL NDKHSNGTIK
160 170 180 190 200
DRSPYRTLMS CPIGEVPSPY NSRFESVAWS ASACHDGINW LTIGISGPDN
210 220 230 240 250
GAVAVLKYNG IITDTIKSWR NNILRTQESE CACVNGSCFT VMTDGPSNGQ
260 270 280 290 300
ASYKIFRIEK GKIVKSVEMN APNYHYEECS CYPDSSEITC VCRDNWHGSN
310 320 330 340 350
RPWVSFNQNL EYQIGYICSG IFGDNPRPND KTGSCGPVSS NGANGVKGFS
360 370 380 390 400
FKYGNGVWIG RTKSISSRNG FEMIWDPNGW TGTDNNFSIK QDIVGINEWS
410 420 430 440 450
GYSGSFVQHP ELTGLDCIRP CFWVELIRGR PKENTIWTSG SSISFCGVNS
460
DTVGWSWPDG AELPFTIDK
Length:469
Mass (Da):51,597
Last modified:June 16, 2009 - v1
Checksum:i24712B3623B0E964
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ966084 Viral cRNA Translation: ACP41107.1
FJ969517 Viral cRNA Translation: ACP44158.1
JF915186 Viral cRNA Translation: AEE69012.1

Entry informationi

Entry nameiC3W5S3_I09A0
AccessioniPrimary (citable) accession number: C3W5S3
Entry historyiIntegrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteomeImported

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