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C3W5S1

- C3W5S1_I09A0

UniProt

C3W5S1 - C3W5S1_I09A0

Protein
Submitted name:

Hemagglutinin

Gene

HA

Organism
Influenza A virus (strain swl A/California/04/2009 H1N1)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei204 – 2041GalactoseImported

    GO - Biological processi

    1. clathrin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
    2. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
    3. fusion of virus membrane with host plasma membrane Source: InterPro
    4. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    HemagglutininUniRule annotationSAAS annotation

    Keywords - Biological processi

    Clathrin- and caveolin-independent endocytosis of virus by hostSAAS annotation, Clathrin-mediated endocytosis of virus by hostSAAS annotation, Fusion of virus membrane with host endosomal membraneSAAS annotation, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cellSAAS annotation, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    HemagglutininImported
    Gene namesi
    Name:HAImported
    OrganismiInfluenza A virus (strain swl A/California/04/2009 H1N1)Imported
    Taxonomic identifieri641501 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    Virion membrane SAAS annotation; Single-pass type I membrane protein SAAS annotation. Host apical cell membrane SAAS annotation; Single-pass type I membrane protein SAAS annotation

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membraneSAAS annotation, Host membrane, Membrane, Viral envelope proteinUniRule annotationSAAS annotation, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717 PotentialImportedAdd
    BLAST
    Chaini18 – 566549 PotentialImportedPRO_5000464216Add
    BLAST
    Chaini18 – 344327HA1ImportedPRO_5000464217Add
    BLAST
    Chaini345 – 566222HA2ImportedPRO_5000464218Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi28 – 281N-linked (GlcNAc...)Imported
    Glycosylationi40 – 401N-linked (GlcNAc...)Imported
    Glycosylationi104 – 1041N-linked (GlcNAc...)Imported
    Glycosylationi293 – 2931N-linked (GlcNAc...)Imported
    Glycosylationi304 – 3041N-linked (GlcNAc...)
    Glycosylationi498 – 4981N-linked (GlcNAc...)Imported

    Keywords - PTMi

    Disulfide bondSAAS annotation

    Interactioni

    Subunit structurei

    Homotrimer of disulfide-linked HA1-HA2.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3AL4X-ray2.87A/C/E/G/I/K18-344[»]
    B/D/F/H/J/L345-520[»]
    3LZGX-ray2.60A/C/E/G/I/K18-344[»]
    B/D/F/H/J/L345-518[»]
    3UBEX-ray2.15A/C/E/G/I/K18-344[»]
    B/D/F/H/J/L345-518[»]
    3UBJX-ray2.25A/C/E/G/I/K18-344[»]
    B/D/F/H/J/L345-518[»]
    3UBNX-ray2.51A/C/E/G/I/K18-344[»]
    B/D/F/H/J/L345-518[»]
    3UBQX-ray2.00A/C/E/G/I/K18-344[»]
    B/D/F/H/J/L345-518[»]
    3UYWX-ray1.90A/B/C/D65-278[»]
    3UYXX-ray1.80A/B65-278[»]
    3ZTNX-ray3.00A18-344[»]
    B345-520[»]
    4JTVX-ray3.00A/C/E/G/I/K18-338[»]
    B/D/F/H/J/L345-506[»]
    4JTXX-ray3.00A/C/E/G/I/K18-339[»]
    B/D/F/H/J/L345-510[»]
    4JU0X-ray2.91A/C/E/G/I/K18-339[»]
    B/D/F/H/J/L345-508[»]
    4M4YX-ray2.20A/C/E18-344[»]
    B/D/F345-518[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiC3W5S1.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni236 – 2394Galactose bindingImported

    Sequence similaritiesi

    Belongs to the influenza viruses hemagglutinin family.UniRule annotation

    Keywords - Domaini

    SignalImported, Transmembrane, Transmembrane helixSAAS annotation

    Family and domain databases

    Gene3Di2.10.77.10. 1 hit.
    3.90.20.10. 1 hit.
    3.90.209.20. 1 hit.
    InterProiIPR008980. Capsid_hemagglutn.
    IPR013828. Hemagglutn_HA1_a/b_dom.
    IPR013827. Hemagglutn_HA1_b-rbn_dom.
    IPR000149. Hemagglutn_influenz_A.
    IPR001364. Hemagglutn_influenz_A/B.
    IPR013829. Hemagglutn_stalk.
    [Graphical view]
    PfamiPF00509. Hemagglutinin. 1 hit.
    [Graphical view]
    PRINTSiPR00330. HEMAGGLUTN1.
    PR00329. HEMAGGLUTN12.
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C3W5S1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKAILVVLLY TFATANADTL CIGYHANNST DTVDTVLEKN VTVTHSVNLL    50
    EDKHNGKLCK LRGVAPLHLG KCNIAGWILG NPECESLSTA SSWSYIVETP 100
    SSDNGTCYPG DFIDYEELRE QLSSVSSFER FEIFPKTSSW PNHDSNKGVT 150
    AACPHAGAKS FYKNLIWLVK KGNSYPKLSK SYINDKGKEV LVLWGIHHPS 200
    TSADQQSLYQ NADTYVFVGS SRYSKKFKPE IAIRPKVRDQ EGRMNYYWTL 250
    VEPGDKITFE ATGNLVVPRY AFAMERNAGS GIIISDTPVH DCNTTCQTPK 300
    GAINTSLPFQ NIHPITIGKC PKYVKSTKLR LATGLRNIPS IQSRGLFGAI 350
    AGFIEGGWTG MVDGWYGYHH QNEQGSGYAA DLKSTQNAID EITNKVNSVI 400
    EKMNTQFTAV GKEFNHLEKR IENLNKKVDD GFLDIWTYNA ELLVLLENER 450
    TLDYHDSNVK NLYEKVRSQL KNNAKEIGNG CFEFYHKCDN TCMESVKNGT 500
    YDYPKYSEEA KLNREEIDGV KLESTRIYQI LAIYSTVASS LVLVVSLGAI 550
    SFWMCSNGSL QCRICI 566
    Length:566
    Mass (Da):63,276
    Last modified:June 16, 2009 - v1
    Checksum:iFBCE01FBF1FCA445
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ966082 Viral cRNA. Translation: ACP41105.1.
    GQ117044 Viral cRNA. Translation: ACQ76318.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ966082 Viral cRNA. Translation: ACP41105.1 .
    GQ117044 Viral cRNA. Translation: ACQ76318.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3AL4 X-ray 2.87 A/C/E/G/I/K 18-344 [» ]
    B/D/F/H/J/L 345-520 [» ]
    3LZG X-ray 2.60 A/C/E/G/I/K 18-344 [» ]
    B/D/F/H/J/L 345-518 [» ]
    3UBE X-ray 2.15 A/C/E/G/I/K 18-344 [» ]
    B/D/F/H/J/L 345-518 [» ]
    3UBJ X-ray 2.25 A/C/E/G/I/K 18-344 [» ]
    B/D/F/H/J/L 345-518 [» ]
    3UBN X-ray 2.51 A/C/E/G/I/K 18-344 [» ]
    B/D/F/H/J/L 345-518 [» ]
    3UBQ X-ray 2.00 A/C/E/G/I/K 18-344 [» ]
    B/D/F/H/J/L 345-518 [» ]
    3UYW X-ray 1.90 A/B/C/D 65-278 [» ]
    3UYX X-ray 1.80 A/B 65-278 [» ]
    3ZTN X-ray 3.00 A 18-344 [» ]
    B 345-520 [» ]
    4JTV X-ray 3.00 A/C/E/G/I/K 18-338 [» ]
    B/D/F/H/J/L 345-506 [» ]
    4JTX X-ray 3.00 A/C/E/G/I/K 18-339 [» ]
    B/D/F/H/J/L 345-510 [» ]
    4JU0 X-ray 2.91 A/C/E/G/I/K 18-339 [» ]
    B/D/F/H/J/L 345-508 [» ]
    4M4Y X-ray 2.20 A/C/E 18-344 [» ]
    B/D/F 345-518 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei C3W5S1.

    Family and domain databases

    Gene3Di 2.10.77.10. 1 hit.
    3.90.20.10. 1 hit.
    3.90.209.20. 1 hit.
    InterProi IPR008980. Capsid_hemagglutn.
    IPR013828. Hemagglutn_HA1_a/b_dom.
    IPR013827. Hemagglutn_HA1_b-rbn_dom.
    IPR000149. Hemagglutn_influenz_A.
    IPR001364. Hemagglutn_influenz_A/B.
    IPR013829. Hemagglutn_stalk.
    [Graphical view ]
    Pfami PF00509. Hemagglutinin. 1 hit.
    [Graphical view ]
    PRINTSi PR00330. HEMAGGLUTN1.
    PR00329. HEMAGGLUTN12.
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: A/California/04/2009Imported.
    2. "Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans."
      Garten R.J., Davis C.T., Russell C.A., Shu B., Lindstrom S., Balish A., Sessions W.M., Xu X., Skepner E., Deyde V., Okomo-Adhiambo M., Gubareva L., Barnes J., Smith C.B., Emery S.L., Hillman M.J., Rivailler P., Smagala J.
      , de Graaf M., Burke D.F., Fouchier R.A., Pappas C., Alpuche-Aranda C.M., Lopez-Gatell H., Olivera H., Lopez I., Myers C.A., Faix D., Blair P.J., Yu C., Keene K.M., Dotson P.D.Jr., Boxrud D., Sambol A.R., Abid S.H., St George K., Bannerman T., Moore A.L., Stringer D.J., Blevins P., Demmler-Harrison G.J., Ginsberg M., Kriner P., Waterman S., Smole S., Guevara H.F., Belongia E.A., Clark P.A., Beatrice S.T., Donis R., Katz J., Finelli L., Bridges C.B., Shaw M., Jernigan D.B., Uyeki T.M., Smith D.J., Klimov A.I., Cox N.J.
      Science 325:197-201(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: A/California/04/2009Imported.
    3. "Human infection with novel swine H1N1 influenza."
      Shu B., Balish A., Garten R., Smith C., Emery S., Barnes J., Deyde V., Klimov A., Cox N.
      Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: A/California/04/2009Imported.
    4. "Crystal structure of the swine-origin A (H1N1)-2009 influenza A virus hemagglutinin (HA) reveals similar antigenicity to that of the 1918 pandemic virus."
      Zhang W., Qi J., Shi Y., Li Q., Gao F., Sun Y., Lu X., Lu Q., Vavricka C.J., Liu D., Yan J., Gao G.F.
      Protein Cell 1:459-467(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.87 ANGSTROMS) OF 18-344 AND 345-520, GLYCOSYLATION AT ASN-40; ASN-104; ASN-293 AND ASN-304.
    5. "Structural basis of preexisting immunity to the 2009 H1N1 pandemic influenza virus."
      Xu R., Ekiert D.C., Krause J.C., Hai R., Crowe J.E., Wilson I.A.
      Science 328:357-360(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 18-344 AND 345-518, GLYCOSYLATION AT ASN-104 AND ASN-293.
    6. "Structural vaccinology: structure-based design of influenza A virus hemagglutinin subtype-specific subunit vaccines."
      Xuan C., Shi Y., Qi J., Zhang W., Xiao H., Gao G.F.
      Protein Cell 2:997-1005(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 65-278.
    7. Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 18-344 AND 345-520, GLYCOSYLATION AT ASN-40 AND ASN-293.
    8. "Structural characterization of the hemagglutinin receptor specificity from the 2009 H1N1 influenza pandemic."
      Xu R., McBride R., Nycholat C.M., Paulson J.C., Wilson I.A.
      J. Virol. 86:982-990(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 18-344 AND 345-518 IN COMPLEX WITH GALACTOSE, GLYCOSYLATION AT ASN-28; ASN-40; ASN-104; ASN-293; ASN-304 AND ASN-498.
    9. "Molecular basis of the receptor binding specificity switch of the hemagglutinins from both the 1918 and 2009 pandemic influenza A viruses by a D225G substitution."
      Zhang W., Shi Y., Qi J., Gao F., Li Q., Fan Z., Yan J., Gao G.F.
      J. Virol. 87:5949-5958(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.91 ANGSTROMS) OF 18-339 AND 345-508 IN COMPLEX WITH GALACTOSE, GLYCOSYLATION AT ASN-28; ASN-40; ASN-104 AND ASN-293.
    10. "Antibody recognition of the pandemic H1N1 Influenza virus hemagglutinin receptor binding site."
      Hong M., Lee P.S., Hoffman R.M., Zhu X., Krause J.C., Laursen N.S., Yoon S.I., Song L., Tussey L., Crowe J.E., Ward A.B., Wilson I.A.
      J. Virol. 87:12471-12480(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 18-344 AND 345-518, GLYCOSYLATION AT ASN-28; ASN-40; ASN-104 AND ASN-293.

    Entry informationi

    Entry nameiC3W5S1_I09A0
    AccessioniPrimary (citable) accession number: C3W5S1
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 16, 2009
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3