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Protein

Hemagglutinin

Gene

HA

Organism
Influenza A virus (strain swl A/California/04/2009 H1N1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei204GalactoseCombined sources1

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

HemagglutininUniRule annotationSAAS annotation

Keywords - Biological processi

Clathrin- and caveolin-independent endocytosis of virus by hostSAAS annotation, Clathrin-mediated endocytosis of virus by hostSAAS annotation, Fusion of virus membrane with host endosomal membraneSAAS annotation, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cellSAAS annotation, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
HemagglutininSAAS annotation
Gene namesi
Name:HAImported
OrganismiInfluenza A virus (strain swl A/California/04/2009 H1N1)Imported
Taxonomic identifieri641501 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

  • Host apical cell membrane SAAS annotation; Single-pass type I membrane protein SAAS annotation
  • Virion membrane SAAS annotation; Single-pass type I membrane protein SAAS annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei531 – 554HelicalSequence analysisAdd BLAST24

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membraneSAAS annotation, Host membrane, Membrane, Viral envelope proteinUniRule annotationSAAS annotation, Virion

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi21Interchain (with C-481)Combined sources
GlycosylationiCAR_500795172228N-linked (GlcNAc...)Combined sources1
GlycosylationiCAR_500795172640N-linked (GlcNAc...)Combined sources1
Disulfide bondi59 ↔ 292Combined sources
Disulfide bondi72 ↔ 84Combined sources
GlycosylationiCAR_5007951725104N-linked (GlcNAc...)Combined sources1
Disulfide bondi107 ↔ 153Combined sources
GlycosylationiCAR_5007951724293N-linked (GlcNAc...)Combined sources1
Disulfide bondi296 ↔ 320Combined sources
GlycosylationiCAR_5007951721304N-linked (GlcNAc...)Combined sources1
Disulfide bondi481Interchain (with C-21)Combined sources
Disulfide bondi488 ↔ 492Combined sources
GlycosylationiCAR_5007951723498N-linked (GlcNAc...)Combined sources1

Keywords - PTMi

Disulfide bondSAAS annotation

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HA1-HA2.UniRule annotationSAAS annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AL4X-ray2.87A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-520[»]
3LZGX-ray2.60A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-518[»]
3UBEX-ray2.15A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-518[»]
3UBJX-ray2.25A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-518[»]
3UBNX-ray2.51A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-518[»]
3UBQX-ray2.00A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-518[»]
3UYWX-ray1.90A/B/C/D65-278[»]
3UYXX-ray1.80A/B65-278[»]
3ZTNX-ray3.00A18-344[»]
B345-520[»]
4JTVX-ray3.00A/C/E/G/I/K18-338[»]
B/D/F/H/J/L345-506[»]
4JTXX-ray3.00A/C/E/G/I/K18-339[»]
B/D/F/H/J/L345-510[»]
4JU0X-ray2.91A/C/E/G/I/K18-339[»]
B/D/F/H/J/L345-508[»]
4M4YX-ray2.20A/C/E18-344[»]
B/D/F345-518[»]
SMRiC3W5S1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 239Galactose bindingCombined sources4

Sequence similaritiesi

Belongs to the influenza viruses hemagglutinin family.UniRule annotationSAAS annotation

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysisSAAS annotation

Family and domain databases

Gene3Di2.10.77.10. 1 hit.
3.90.20.10. 1 hit.
3.90.209.20. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom.
IPR013827. Hemagglutn_HA1_b-rbn_dom.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
IPR013829. Hemagglutn_stalk.
[Graphical view]
PfamiPF00509. Hemagglutinin. 1 hit.
[Graphical view]
PRINTSiPR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C3W5S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAILVVLLY TFATANADTL CIGYHANNST DTVDTVLEKN VTVTHSVNLL
60 70 80 90 100
EDKHNGKLCK LRGVAPLHLG KCNIAGWILG NPECESLSTA SSWSYIVETP
110 120 130 140 150
SSDNGTCYPG DFIDYEELRE QLSSVSSFER FEIFPKTSSW PNHDSNKGVT
160 170 180 190 200
AACPHAGAKS FYKNLIWLVK KGNSYPKLSK SYINDKGKEV LVLWGIHHPS
210 220 230 240 250
TSADQQSLYQ NADTYVFVGS SRYSKKFKPE IAIRPKVRDQ EGRMNYYWTL
260 270 280 290 300
VEPGDKITFE ATGNLVVPRY AFAMERNAGS GIIISDTPVH DCNTTCQTPK
310 320 330 340 350
GAINTSLPFQ NIHPITIGKC PKYVKSTKLR LATGLRNIPS IQSRGLFGAI
360 370 380 390 400
AGFIEGGWTG MVDGWYGYHH QNEQGSGYAA DLKSTQNAID EITNKVNSVI
410 420 430 440 450
EKMNTQFTAV GKEFNHLEKR IENLNKKVDD GFLDIWTYNA ELLVLLENER
460 470 480 490 500
TLDYHDSNVK NLYEKVRSQL KNNAKEIGNG CFEFYHKCDN TCMESVKNGT
510 520 530 540 550
YDYPKYSEEA KLNREEIDGV KLESTRIYQI LAIYSTVASS LVLVVSLGAI
560
SFWMCSNGSL QCRICI
Length:566
Mass (Da):63,276
Last modified:June 16, 2009 - v1
Checksum:iFBCE01FBF1FCA445
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ966082 Viral cRNA. Translation: ACP41105.1.
GQ117044 Viral cRNA. Translation: ACQ76318.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ966082 Viral cRNA. Translation: ACP41105.1.
GQ117044 Viral cRNA. Translation: ACQ76318.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AL4X-ray2.87A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-520[»]
3LZGX-ray2.60A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-518[»]
3UBEX-ray2.15A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-518[»]
3UBJX-ray2.25A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-518[»]
3UBNX-ray2.51A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-518[»]
3UBQX-ray2.00A/C/E/G/I/K18-344[»]
B/D/F/H/J/L345-518[»]
3UYWX-ray1.90A/B/C/D65-278[»]
3UYXX-ray1.80A/B65-278[»]
3ZTNX-ray3.00A18-344[»]
B345-520[»]
4JTVX-ray3.00A/C/E/G/I/K18-338[»]
B/D/F/H/J/L345-506[»]
4JTXX-ray3.00A/C/E/G/I/K18-339[»]
B/D/F/H/J/L345-510[»]
4JU0X-ray2.91A/C/E/G/I/K18-339[»]
B/D/F/H/J/L345-508[»]
4M4YX-ray2.20A/C/E18-344[»]
B/D/F345-518[»]
SMRiC3W5S1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.10.77.10. 1 hit.
3.90.20.10. 1 hit.
3.90.209.20. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom.
IPR013827. Hemagglutn_HA1_b-rbn_dom.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
IPR013829. Hemagglutn_stalk.
[Graphical view]
PfamiPF00509. Hemagglutinin. 1 hit.
[Graphical view]
PRINTSiPR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMiSSF49818. SSF49818. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiC3W5S1_I09A0
AccessioniPrimary (citable) accession number: C3W5S1
Entry historyi
Integrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.