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Protein

Polymerase acidic protein

Gene

PA

Organism
Influenza A virus (strain swl A/California/04/2009 H1N1)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Manganese 1; via tele nitrogenUniRule annotationCombined sources1
Metal bindingi80Manganese 2UniRule annotation1
Metal bindingi108Manganese 1UniRule annotationCombined sources1
Metal bindingi108Manganese 2UniRule annotationCombined sources1
Metal bindingi119Manganese 1UniRule annotation1
Metal bindingi119Manganese 2Combined sources1
Metal bindingi120Manganese 1; via carbonyl oxygenUniRule annotationCombined sources1
Metal bindingi120Manganese 2; via carbonyl oxygenCombined sources1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonucleaseUniRule annotationSAAS annotation, Hydrolase, Nuclease
Biological processCap snatchingUniRule annotationSAAS annotation, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virusUniRule annotationSAAS annotation
LigandManganeseUniRule annotationCombined sourcesSAAS annotation, Metal-bindingUniRule annotationCombined sourcesSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase acidic proteinUniRule annotationSAAS annotation (EC:3.1.-.-UniRule annotation)
Alternative name(s):
RNA-directed RNA polymerase subunit P2UniRule annotation
Gene namesi
Name:PAUniRule annotationImported
OrganismiInfluenza A virus (strain swl A/California/04/2009 H1N1)Imported
Taxonomic identifieri641501 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000132424 Componenti: Genome

Subcellular locationi

  • Host nucleus SAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasmUniRule annotationSAAS annotation, Host nucleusUniRule annotationSAAS annotation

PTM / Processingi

Post-translational modificationi

Phosphorylated on serines and threonines by host kinases, including human casein kinase II.UniRule annotation

Keywords - PTMi

PhosphoproteinUniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AVGX-ray2.20A/B/C/D1-198[»]
4AVLX-ray1.87A/B/C/D1-198[»]
4AVQX-ray2.10A/B/C/D1-198[»]
4AWFX-ray2.30A/B/C/D1-198[»]
4AWGX-ray2.60A/B/C/D1-198[»]
4AWHX-ray2.05A/B/C/D1-198[»]
4AWKX-ray1.90A1-198[»]
4AWMX-ray2.60A1-198[»]
5CCYX-ray2.10A1-50[»]
A73-196[»]
5CGVX-ray2.17A1-50[»]
A73-196[»]
5CL0X-ray2.22A1-50[»]
A73-196[»]
5CZNX-ray1.98A1-50[»]
A73-196[»]
5D2OX-ray2.15A1-50[»]
A73-196[»]
5D42X-ray2.00A1-50[»]
A73-196[»]
5D4GX-ray2.08A1-50[»]
A73-196[»]
5D8UX-ray2.29A1-50[»]
A73-196[»]
5D9JX-ray1.85A1-50[»]
A73-196[»]
5DBSX-ray2.11A1-50[»]
A73-196[»]
5DEBX-ray2.14A1-50[»]
A73-196[»]
5DESX-ray2.05A1-50[»]
A73-196[»]
5EGAX-ray2.15A1-50[»]
A73-196[»]
5W3IX-ray1.95A1-50[»]
A73-196[»]
5W44X-ray2.10A1-50[»]
A73-196[»]
5W73X-ray2.20A1-50[»]
A73-196[»]
5W7UX-ray2.20A1-50[»]
A73-196[»]
5W9GX-ray2.10A1-50[»]
A73-196[»]
5WA7X-ray2.20A1-50[»]
A73-196[»]
5WAPX-ray2.20A1-50[»]
A73-196[»]
5WB3X-ray2.20A1-50[»]
A73-196[»]
5WCSX-ray2.52A1-50[»]
A73-196[»]
5WCTX-ray2.30A1-50[»]
A73-196[»]
5WDCX-ray2.10A1-50[»]
A73-196[»]
5WDNX-ray2.10A1-50[»]
A73-196[»]
5WDWX-ray2.30A1-50[»]
A73-196[»]
5WE7X-ray2.12A1-50[»]
A73-196[»]
5WE9X-ray1.80A1-50[»]
A73-196[»]
5WEBX-ray2.25A1-50[»]
A73-196[»]
5WEFX-ray2.00A1-50[»]
A73-196[»]
5WEIX-ray2.25A1-50[»]
A73-196[»]
5WF3X-ray2.25A1-50[»]
A73-196[»]
5WFMX-ray2.25A1-50[»]
A73-196[»]
5WFWX-ray2.29A1-50[»]
A73-196[»]
5WFZX-ray2.35A1-50[»]
A73-196[»]
5WG9X-ray2.30A1-50[»]
A73-196[»]
SMRiC3W5S0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi184 – 247Nuclear localization signal 2 (NLS2)UniRule annotationAdd BLAST64

Sequence similaritiesi

Belongs to the influenza viruses PA family.UniRule annotationSAAS annotation

Family and domain databases

Gene3Di3.40.91.90. 1 hit.
HAMAPiMF_04063. INFV_PA. 1 hit.
InterProiView protein in InterPro
IPR037534. INFV_PA.
IPR001009. PA/PA-X.
IPR038372. PA/PA-X_sf.
PfamiView protein in Pfam
PF00603. Flu_PA. 1 hit.

Sequencei

Sequence statusi: Complete.

C3W5S0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDFVRQCFN PMIVELAEKA MKEYGEDPKI ETNKFAAICT HLEVCFMYSD
60 70 80 90 100
FHFIDERGES IIVESGDPNA LLKHRFEIIE GRDRIMAWTV VNSICNTTGV
110 120 130 140 150
EKPKFLPDLY DYKENRFIEI GVTRREVHIY YLEKANKIKS EKTHIHIFSF
160 170 180 190 200
TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRSLWDS FRQSERGEET
210 220 230 240 250
IEEKFEITGT MRKLADQSLP PNFPSLENFR AYVDGFEPNG CIEGKLSQMS
260 270 280 290 300
KEVNAKIEPF LRTTPRPLRL PDGPLCHQRS KFLLMDALKL SIEDPSHEGE
310 320 330 340 350
GIPLYDAIKC MKTFFGWKEP NIVKPHEKGI NPNYLMAWKQ VLAELQDIEN
360 370 380 390 400
EEKIPRTKNM KRTSQLKWAL GENMAPEKVD FDDCKDVGDL KQYDSDEPEP
410 420 430 440 450
RSLASWVQNE FNKACELTDS SWIELDEIGE DVAPIEHIAS MRRNYFTAEV
460 470 480 490 500
SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL
510 520 530 540 550
YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEIGDMLL
560 570 580 590 600
RTAIGQVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES
610 620 630 640 650
SVKEKDMTKE FFENKSETWP IGESPRGVEE GSIGKVCRTL LAKSVFNSLY
660 670 680 690 700
ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV
710
LLNASWFNSF LTHALK
Length:716
Mass (Da):82,656
Last modified:June 16, 2009 - v1
Checksum:iD654A8B19844D51D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ966081 Viral cRNA. Translation: ACP41104.1.
FJ969515 Viral cRNA. Translation: ACP44156.1.
JF915188 Viral cRNA. Translation: AEE69014.1.

Keywords - Coding sequence diversityi

Ribosomal frameshiftingUniRule annotationSAAS annotation

Similar proteinsi

Entry informationi

Entry nameiC3W5S0_I09A0
AccessioniPrimary (citable) accession number: C3W5S0
Entry historyiIntegrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

3D-structureCombined sources, Complete proteomeImported