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Protein

Protein NLRC5

Gene

Nlrc5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable regulator of the NF-kappa-B and type I interferon signaling pathways. May also regulate the type II interferon signaling pathway. Plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi229 – 2368ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NLRC5
Gene namesi
Name:Nlrc5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:3612191. Nlrc5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: BHF-UCL
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19151915Protein NLRC5PRO_0000397867Add
BLAST

Proteomic databases

EPDiC3VPR6.
MaxQBiC3VPR6.
PeptideAtlasiC3VPR6.
PRIDEiC3VPR6.

PTM databases

iPTMnetiC3VPR6.

Expressioni

Tissue specificityi

Expressed in spleen, thymus and lung.1 Publication

Inductioni

By interferon gamma. By LPS and viruses (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiC3VPR6. baseline and differential.
GenevisibleiC3VPR6. MM.

Interactioni

Subunit structurei

Interacts with CHUK and IKBKB; prevents CHUK and IKBKB phosphorylation and inhibits their kinase activity. Interacts with DDX58 and IFIH1; blocks the interaction of MAVS to DDX58.By similarity

Protein-protein interaction databases

IntActiC3VPR6. 2 interactions.

Structurei

Secondary structure

1
1915
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 43Combined sources
Helixi11 – 2212Combined sources
Helixi25 – 3511Combined sources
Beta strandi45 – 484Combined sources
Helixi50 – 6314Combined sources
Helixi65 – 7814Combined sources
Helixi86 – 905Combined sources
Helixi91 – 933Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MJMNMR-A1-96[»]
ProteinModelPortaliC3VPR6.
SMRiC3VPR6. Positions 1-96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini223 – 542320NACHTPROSITE-ProRule annotationAdd
BLAST
Repeati622 – 64625LRR 1Add
BLAST
Repeati716 – 74025LRR 2Add
BLAST
Repeati744 – 77128LRR 3Add
BLAST
Repeati772 – 79625LRR 4Add
BLAST
Repeati871 – 89828LRR 5Add
BLAST
Repeati900 – 92324LRR 6Add
BLAST
Repeati930 – 95324LRR 7Add
BLAST
Repeati1006 – 103328LRR 8Add
BLAST
Repeati1034 – 105522LRR 9Add
BLAST
Repeati1138 – 116124LRR 10Add
BLAST
Repeati1162 – 118423LRR 11Add
BLAST
Repeati1240 – 126324LRR 12Add
BLAST
Repeati1265 – 129228LRR 13Add
BLAST
Repeati1348 – 137124LRR 14Add
BLAST
Repeati1481 – 150424LRR 15Add
BLAST
Repeati1519 – 154224LRR 16Add
BLAST
Repeati1552 – 157524LRR 17Add
BLAST
Repeati1576 – 159823LRR 18Add
BLAST
Repeati1603 – 162624LRR 19Add
BLAST
Repeati1631 – 165424LRR 20Add
BLAST
Repeati1659 – 168224LRR 21Add
BLAST
Repeati1687 – 171125LRR 22Add
BLAST
Repeati1715 – 173824LRR 23Add
BLAST
Repeati1741 – 176828LRR 24Add
BLAST
Repeati1769 – 179527LRR 25Add
BLAST
Repeati1821 – 184525LRR 26Add
BLAST
Repeati1849 – 187224LRR 27Add
BLAST

Sequence similaritiesi

Belongs to the NLRP family.Curated
Contains 27 LRR (leucine-rich) repeats.Curated
Contains 1 NACHT domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

GeneTreeiENSGT00840000129782.
HOGENOMiHOG000168392.
HOVERGENiHBG108194.
InParanoidiC3VPR6.
OMAiIRLWNNP.
PhylomeDBiC3VPR6.

Family and domain databases

Gene3Di3.80.10.10. 6 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR007111. NACHT_NTPase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13516. LRR_6. 7 hits.
[Graphical view]
SMARTiSM00367. LRR_CC. 13 hits.
SM00369. LRR_TYP. 9 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50837. NACHT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: C3VPR6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAESIRLNN ENLWAWLVRL LSKNPEWLSA KLRSFLPTMD LDCSYEPSNP
60 70 80 90 100
EVIHRQLNRL FAQGMATWKS FINDLCFELD VPLDMEIPLV SIWGPRDEFS
110 120 130 140 150
KQLGAGEESC PGPQLYHGAK RPFQSYGSSP RRKNSKKQQL ELAKKYLKLL
160 170 180 190 200
KTSAQQWHGG VCPGAWLTPH SPQTYIPPVL QWSRATAPLD AQEGATLGDP
210 220 230 240 250
EAADNIDVSI QDLFSFKAHK GPRVTVLLGK AGMGKTTLAY RLRWRWAQGQ
260 270 280 290 300
LDRFQALFLF EFRQLNMITQ LPTLPQLLFD LYLMPESEPD AVFQYLKENA
310 320 330 340 350
QEVLLIFDGL DEALHADSVG TDNAGSALTL FSELCHGNLL PGCWVMTTSR
360 370 380 390 400
PGKLPSCVPT EAATVHMWGF DGLRVEKYVT CFFSDLLSQE LALKEMRTNA
410 420 430 440 450
RLRGMCAIPA LCTVTCFCLR RLLPGSSPGQ SAALLPTITQ LYLQMVETFS
460 470 480 490 500
PSETLLDTSI LGFGKVALRG LDTGKVVFSV EDISPQLMSF GAVHSLLTSF
510 520 530 540 550
CIHTRPGHEE IGYAFVHLSL QEFFAALYLM ASHTVDKDTL VEYVTLNSHW
560 570 580 590 600
VLRTKGRLGL SDHLPAFLAG LASHTCHMFL CQLAQQDRAW VGSRQAAVIQ
610 620 630 640 650
VLRKLASRKL TGPKMIELYH CVAETQDLEL ARFTAQSLPS RLSFHNFPLT
660 670 680 690 700
HADLAALANI LEHRDDPIHL DFDGCPLEPH CPEALVGCGQ VENLSFKSRK
710 720 730 740 750
CGDAFAEALC RSLPTMGSLK TLGLTGSRIT AQGISHLIQT LPLCSQLEEV
760 770 780 790 800
SLHDNQLKDP EVLSLVELLP SLPKLQKLDL SRNSFSRSIL LSLVKVAITC
810 820 830 840 850
PTVRKLQVRE LDLIFYLSPV TETATQQSGA SDVQGKDSLK EGQSRSLQLR
860 870 880 890 900
LQKCQLRIRD AEALVELFQK SPQLEEVNLS GNHLEDDGCR LVAEAASQLH
910 920 930 940 950
IAQKLDLSDN GLSQTGVTYV LKAMSTCGTL EDLHISLLNN TVVLTFAQEP
960 970 980 990 1000
REQEGSCKGR APLISFVSPV TSELSQRSRR IRLTHCGFLA KHTETLCEAL
1010 1020 1030 1040 1050
RASCQTHNLD HLDLSDNSLG GKGVILLTEL LPGLGPLKSL NLSRNGLSMD
1060 1070 1080 1090 1100
AVFSLVQCLS SLQWVFHLDV SLESDCIFLR GAGTSRDALE PKFQTGVQVL
1110 1120 1130 1140 1150
ELSQRYTSRS FCLQECQLEP TSLTFLCATL EKSPGPLEVQ LSCKSLSDDS
1160 1170 1180 1190 1200
LKILLQCLPQ LPQLSLLQLR HTVLSSRSPF LLADIFNLCP RVRKVTLRSL
1210 1220 1230 1240 1250
CHAVLHFDSN EEQEGVCCGF PGCSLSQEHM ETLCCALSKC NALSQLDLTD
1260 1270 1280 1290 1300
NLLGDIGLRC LLECLPQLPI SGWLDLSHNN ISQEGILYLL ETLPSYPNIQ
1310 1320 1330 1340 1350
EVSVSLSSEQ IFRMCFSKKE GAGTSLRLCE CSFSPEQVSK LASSLSQAQQ
1360 1370 1380 1390 1400
LTELWLTKCH LDLPQLTMLL NLVNRPTGLL GLRLEEPWVD SVSLPALMEV
1410 1420 1430 1440 1450
CAQASGCLTE LSISEIQRKL WLQLEFPHQE GNSDSMALRL AHCDLETEHS
1460 1470 1480 1490 1500
HLMIQLVETY ARLQQLSLSQ VSFNDNDGTS SKLLQNILLS SCELKSFRLT
1510 1520 1530 1540 1550
FSQVSTKSLT HLAFGLGHCH HLEELDFSNN SLREEDTELL MGALQGTCRL
1560 1570 1580 1590 1600
KKLHLSFLPL GASSLALLIQ GLSRMTLLQD LCLSHNQIGD VGTQCLAAIL
1610 1620 1630 1640 1650
PKLPELRKFD LSHNQIGDVG TQCLAAILPK LPELRKFNLS HNQIGHVGTQ
1660 1670 1680 1690 1700
CLAAILPKLP ELRKFDLSRN QIGDVGTQCL AAILPKLPEL RKFDLSGNRI
1710 1720 1730 1740 1750
GPAGGVQLVK SLTHFEHLEE IKLGNNALGE PTALELAQRL PPQLRVLCLP
1760 1770 1780 1790 1800
SSHLGPEGAL GLAQALEQCP HIEEVSLAEN NLAGGVPRFS KRLPLLRQID
1810 1820 1830 1840 1850
LEFCKIEDQA ARHLAANLTL FPALEKLLLS GNLLGDEVAA ELAQVLPQMG
1860 1870 1880 1890 1900
QLKKVNLEWN RITARGAQLL AQGLVQGSCV PVIRLWNNPI LNDVAQSLQS
1910
QEPRLDFSIT DQQTL
Length:1,915
Mass (Da):211,710
Last modified:October 5, 2010 - v2
Checksum:i731919C80F353C1F
GO
Isoform 2 (identifier: C3VPR6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1384-1392: LEEPWVDSV → YLPALGRRV
     1393-1915: Missing.

Note: Due to intron retention. No experimental confirmation available.
Show »
Length:1,392
Mass (Da):154,279
Checksum:i9C778B0056FD1050
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti489 – 4902SF → AS in BAD90135 (PubMed:12465718).Curated
Sequence conflicti578 – 5781M → T in BAD90135 (PubMed:12465718).Curated
Sequence conflicti641 – 6411R → C in BAD90135 (PubMed:12465718).Curated
Sequence conflicti646 – 6461N → K in BAD90135 (PubMed:12465718).Curated
Sequence conflicti833 – 8331V → I in BAD90135 (PubMed:12465718).Curated
Sequence conflicti881 – 8811G → R in ACP40992 (PubMed:20434986).Curated
Sequence conflicti896 – 8961A → T in BAD90135 (PubMed:12465718).Curated
Sequence conflicti939 – 9391N → S in BAD90135 (PubMed:12465718).Curated
Sequence conflicti1105 – 11051R → Q in BAD90135 (PubMed:12465718).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1384 – 13929LEEPWVDSV → YLPALGRRV in isoform 2. 1 PublicationVSP_039702
Alternative sequencei1393 – 1915523Missing in isoform 2. 1 PublicationVSP_039703Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ889356 mRNA. Translation: ACP40992.1.
AK220210 mRNA. Translation: BAD90135.1.
CCDSiCCDS59741.1. [C3VPR6-1]
RefSeqiNP_001028379.2. NM_001033207.3. [C3VPR6-1]
XP_006531225.1. XM_006531162.2. [C3VPR6-1]
XP_006531226.1. XM_006531163.2. [C3VPR6-1]
XP_011246730.1. XM_011248428.1. [C3VPR6-1]
UniGeneiMm.334720.

Genome annotation databases

EnsembliENSMUST00000053085; ENSMUSP00000138322; ENSMUSG00000074151. [C3VPR6-1]
GeneIDi434341.
KEGGimmu:434341.
UCSCiuc009mwj.2. mouse. [C3VPR6-2]
uc012gio.1. mouse. [C3VPR6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ889356 mRNA. Translation: ACP40992.1.
AK220210 mRNA. Translation: BAD90135.1.
CCDSiCCDS59741.1. [C3VPR6-1]
RefSeqiNP_001028379.2. NM_001033207.3. [C3VPR6-1]
XP_006531225.1. XM_006531162.2. [C3VPR6-1]
XP_006531226.1. XM_006531163.2. [C3VPR6-1]
XP_011246730.1. XM_011248428.1. [C3VPR6-1]
UniGeneiMm.334720.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MJMNMR-A1-96[»]
ProteinModelPortaliC3VPR6.
SMRiC3VPR6. Positions 1-96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiC3VPR6. 2 interactions.

PTM databases

iPTMnetiC3VPR6.

Proteomic databases

EPDiC3VPR6.
MaxQBiC3VPR6.
PeptideAtlasiC3VPR6.
PRIDEiC3VPR6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053085; ENSMUSP00000138322; ENSMUSG00000074151. [C3VPR6-1]
GeneIDi434341.
KEGGimmu:434341.
UCSCiuc009mwj.2. mouse. [C3VPR6-2]
uc012gio.1. mouse. [C3VPR6-1]

Organism-specific databases

CTDi84166.
MGIiMGI:3612191. Nlrc5.

Phylogenomic databases

GeneTreeiENSGT00840000129782.
HOGENOMiHOG000168392.
HOVERGENiHBG108194.
InParanoidiC3VPR6.
OMAiIRLWNNP.
PhylomeDBiC3VPR6.

Miscellaneous databases

PROiC3VPR6.
SOURCEiSearch...

Gene expression databases

ExpressionAtlasiC3VPR6. baseline and differential.
GenevisibleiC3VPR6. MM.

Family and domain databases

Gene3Di3.80.10.10. 6 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR007111. NACHT_NTPase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13516. LRR_6. 7 hits.
[Graphical view]
SMARTiSM00367. LRR_CC. 13 hits.
SM00369. LRR_TYP. 9 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50837. NACHT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NLRC5 negatively regulates the NF-kappaB and type I interferon signaling pathways."
    Cui J., Zhu L., Xia X., Wang H.Y., Legras X., Hong J., Ji J., Shen P., Zheng S., Chen Z.J., Wang R.F.
    Cell 141:483-496(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Strain: BALB/cJ.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
    DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 285-1915 (ISOFORM 2).
    Tissue: Spleen.

Entry informationi

Entry nameiNLRC5_MOUSE
AccessioniPrimary (citable) accession number: C3VPR6
Secondary accession number(s): Q571H9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 5, 2010
Last modified: July 6, 2016
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Supposed to contain a CARD domain at the N-terminus. However, this domain is not detected by Pfam, PROSITE or SMART. Has a weak similarity with a DAPIN domain.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.