C3V005 (C3V005_9FLAV) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Polyprotein EMBL ACO82049.1
Organism	Chimeric Tick-borne encephalitis virus/Dengue virus 4 EMBL ACO82049.1
Taxonomic identifier	638787 [NCBI]
Taxonomic lineage	Viruses › ssRNA positive-strand viruses, no DNA stage › Flaviviridae › Flavivirus

Protein attributes

Sequence length	3392 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as an homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes By similarity. SAAS SAAS013754
Catalytic activity	ATP + H2O = ADP + phosphate. SAAS SAAS007094 NTP + H2O = NDP + phosphate. SAAS SAAS007094 Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). SAAS SAAS007094
Subcellular location	Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein By similarity SAAS SAAS013754.
Sequence similarities	Contains 1 RdRp catalytic domain. SAAS SAAS007094 Contains 1 helicase ATP-binding domain. SAAS SAAS007094 Contains 1 helicase C-terminal domain. SAAS SAAS007094

Ontologies

Keywords
Biological process	Clathrin-mediated endocytosis of virus by host SAAS SAAS013754 Fusion of virus membrane with host endosomal membrane SAAS SAAS013754 Fusion of virus membrane with host membrane Host-virus interaction Initiation of viral infection RNA replication SAAS SAAS007094 Viral attachment to host cell SAAS SAAS013754 Viral penetration into host cytoplasm Virus endocytosis by host
Cellular component	Host endoplasmic reticulum SAAS SAAS013754 Host membrane SAAS SAAS013754 Membrane Viral envelope protein SAAS SAAS013754 Virion
Domain	Transmembrane Transmembrane helix SAAS SAAS007094
Ligand	ATP-binding Nucleotide-binding
Molecular function	Capsid protein SAAS SAAS007094 Helicase SAAS SAAS007094 Hydrolase Nucleotidyltransferase RNA-directed RNA polymerase SAAS SAAS007094 Transferase
PTM	Disulfide bond SAAS SAAS013754
Technical term	3D-structure PDB 1K4R
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral capsid Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: InterPro virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA helicase activity Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW double-stranded RNA binding Inferred from electronic annotation. Source: InterPro mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro mRNA (nucleoside-2'-O-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type exopeptidase activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C3V005 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 0C7C70F2A34E4418	FASTA	3,392	378,138
	10 20 30 40 50 60 MNQRKKVVRP PFNMLKRERN RVSTPQGLVK RFSTGLFSGK GPLRMVLAFI TFLRVLSIPP 70 80 90 100 110 120 TAGILKRWGQ LKKNKAIKIL IGFRKEIGRM LNILNGRKRS AVDWTGWLLV VVLLGVTLAA 130 140 150 160 170 180 TVRKERDGTT VIRAEGKDAA TQVRVENGTC VILATDMGSW CDDSLTYECV TIDQGEEPVD 190 200 210 220 230 240 VDCFCRNVDG VYLEYGRCGK QEGSRTRRSV LIPSHAQGDL TGRGHKWLEG DSLRTHLTRV 250 260 270 280 290 300 EGWVWKNKVL TLAVIAVVWL TVESVVTRVA VVVVLLCLAP VYASRCTHLE NRDFVTGTQG 310 320 330 340 350 360 TTRVTLVLEL GGCVTITAEG KPSMDVWLDS IYQENPAKTR EYCLHAKLSD TKVAARCPTM 370 380 390 400 410 420 GPATLAEEHQ SGTVCKRDQS DRGWGNHCGL FGKGSIVTCV KASCEAKKKA TGHVYDANKI 430 440 450 460 470 480 VYTVKVEPHT GDYVAANETH SGRKTASFTV SSERTILTMG DYGDVSLLCR VASGVDLAQT 490 500 510 520 530 540 VILELDKTSE HLPTAWQVHR DWFNDLALPW KHEGAQNWNN AERLVEFGAP HAVKMDVYNL 550 560 570 580 590 600 GDQTGVLLKS LAGVPVAHID GTKYHLKSGH VTCEVGLEKL KMKGLTYTMC DKTKFTWKRI 610 620 630 640 650 660 PTDSGHDTVV MEVAFSGTKP CRIPVRAVAH GSPDVNVAML ITPNPTIENN GGGFIEMQLP 670 680 690 700 710 720 PGDNIIYVGE LSHQWFQKGS SIGRVFQKTR KGIERLTVIG EHAWDFGSTG GFLTSVGKAL 730 740 750 760 770 780 HTVLGGAFNS LFGGVGFLPK ILVGVVLAWL GLNSRNTSMA MTCIAVGGIT LFLGFTVQAD 790 800 810 820 830 840 MGCVASWSGK ELKCGSGIFV VDNVHTWTEQ YKFQPESPAR LASAILNAHK DGVCGIRSTT 850 860 870 880 890 900 RLENVMWKQI TNELNYVLWE GGHDLTVVAG DVKGVLTKGK RALTPPVSDL KYSWKTWGKA 910 920 930 940 950 960 KIFTPEARNS TFLIDGPDTS ECPNERRAWN SLEVEDYGFG MFTTNIWMKF REGSSEVCDH 970 980 990 1000 1010 1020 RLMSAAIKDQ KAVHADMGYW IESSKNQTWQ IEKASLIEVK TCLWPKTHTL WSNGVLESQM 1030 1040 1050 1060 1070 1080 LIPKSYAGPF SQHNYRQGYA TQTVGPWHLG KLEIDFGECP GTTVTIQEDC DHRGPSLRTT 1090 1100 1110 1120 1130 1140 TASGKLVTQW CCRSCTMPPL RFLGEDGCWY GMEIRPLSEK EENMVKSQVT AGQGTSETFS 1150 1160 1170 1180 1190 1200 MGLLCLTLFV EECLRRRVTR KHMILVVVIT LCAIILGGLT WMDLLRALIM LGDTMSGRIG 1210 1220 1230 1240 1250 1260 GQIHLAIMAV FKMSPGYVLG VFLRKLTSRE TALMVIGMAM TTVLSIPHDL MELIDGISLG 1270 1280 1290 1300 1310 1320 LILLKIVTQF DNTQVGTLAL SLTFIRSTMP LVMAWRTIMA VLFVVTLIPL CRTSCLQKQS 1330 1340 1350 1360 1370 1380 HWVEITALIL GAQALPVYLM TLMKGASRRS WPLNEGIMAV GLVSLLGSAL LKNDVPLAGP 1390 1400 1410 1420 1430 1440 MVAGGLLLAA YVMSGSSADL SLEKAANVQW DEMADITGSS PIIEVKQDED GSFSIRDVEE 1450 1460 1470 1480 1490 1500 TNMITLLVKL ALITVSGLYP LAIPVTMTLW YMWQVKTQRS GALWDVPSPA ATKKAALSEG 1510 1520 1530 1540 1550 1560 VYRIMQRGLF GKTQVGVGIH MEGVFHTMWH VTRGSVICHE TGRLEPSWAD VRNDMISYGG 1570 1580 1590 1600 1610 1620 GWRLGDKWDK EEDVQVLAIE PGKNPKHVQT KPGLFKTLTG EIGAVTLDFK PGTSGSPIIN 1630 1640 1650 1660 1670 1680 RKGKVIGLYG NGVVTKSGDY VSAITQAERI GEPDYEVDED IFRKKRLTIM DLHPGAGKTK 1690 1700 1710 1720 1730 1740 RILPSIVREA LKRRLRTLIL APTRVVAAEM EEALRGLPIR YQTPAVKSEH TGREIVDLMC 1750 1760 1770 1780 1790 1800 HATFTTRLLS STRVPNYNLI VMDEAHFTDP SSVAARGYIS TRVEMGEAAA IFMTATPPGA 1810 1820 1830 1840 1850 1860 TDPFPQSNSP IEDIEREIPE RSWNTGFDWI TDYQGKTVWF VPSIKAGNDI ANCLRKSGKK 1870 1880 1890 1900 1910 1920 VIQLSRKTFD TEYPKTKLTD WDFVVTTDIS EMGANFRAGR VIDPRRCLKP VILPDGPERV 1930 1940 1950 1960 1970 1980 ILAGPIPVTP ASAAQRRGRI GRNPAQEDDQ YVFSGDPLKN DEDHAHWTEA KMLLDNIYTP 1990 2000 2010 2020 2030 2040 EGIIPTLFGP EREKTQAIDG EFRLRGEQRK TFVELMRRGD LPVWLSYKVA SAGISYKDRE 2050 2060 2070 2080 2090 2100 WCFTGERNNQ ILEENMEVEI WTREGEKKKL RPRWLDARVY ADPMALKDFK EFASGRKSIT 2110 2120 2130 2140 2150 2160 LDILTEIASL PTYLSSRAKL ALDNIVMLHT TERGGRAYQH ALNELPESLE TLMLVALLGA 2170 2180 2190 2200 2210 2220 MTAGIFLFFM QGKGIGKLSM GLITIAVASG LLWVAEIQPQ WIAASIILEF FLMVLLIPEP 2230 2240 2250 2260 2270 2280 EKQRTPQDNQ LIYVILTILT IIGLIAANEM GLIEKTKTDF GFYQVKTETT ILDVDLRPAS 2290 2300 2310 2320 2330 2340 AWTLYAVATT ILTPMLRHTI ENTSANLSLA AIANQAAVLM GLGKGWPLHR MDLGVPLLAM 2350 2360 2370 2380 2390 2400 GCYSQVNPTT LTASLVMLLV HYAIIGPGLQ AKATREAQKR TAAGIMKNPT VDGITVIDLE 2410 2420 2430 2440 2450 2460 PISYDPKFEK QLGQVMLLVL CAGQLLLMRT TWAFCEVLTL ATGPILTLWE GNPGRFWNTT 2470 2480 2490 2500 2510 2520 IAVSTANIFR GSYLAGAGLA FSLIKNAQTP RRGTGTTGET LGEKWKRQLN SLDRKEFEEY 2530 2540 2550 2560 2570 2580 KRSGILEVDR TEAKSALKDG SKIKHAVSRG SSKIRWIVER GMVKPKGKVV DLGCGRGGWS 2590 2600 2610 2620 2630 2640 YYMATLKNVT EVKGYTKGGP GHEEPIPMAT YGWNLVKLHS GVDVFYKPTE QVDTLLCDIG 2650 2660 2670 2680 2690 2700 ESSSNPTIEE GRTLRVLKMV EPWLSSKPEF CIKVLNPYMP TVIEELEKLQ RKHGGNLVRC 2710 2720 2730 2740 2750 2760 PLSRNSTHEM YWVSGASGNI VSSVNTTSKM LLNRFTTRHR KPTYEKDVDL GAGTRSVSTE 2770 2780 2790 2800 2810 2820 TEKPDMTIIG RRLQRLQEEH KETWHYDQEN PYRTWAYHGS YEAPSTGSAS SMVNGVVKLL 2830 2840 2850 2860 2870 2880 TKPWDVIPMV TQLAMTDTTP FGQQRVFKEK VDTRTPQPKP GTRMVMTTTA NWLWALLGKK 2890 2900 2910 2920 2930 2940 KNPRLCTREE FISKVRSNAA IGAVFQEEQG WTSASEAVND SRFWELVDKE RALHQEGKCE 2950 2960 2970 2980 2990 3000 SCVYNMMGKR EKKLGEFGRA KGSRAIWYMW LGARFLEFEA LGFLNEDHWF GRENSWSGVE 3010 3020 3030 3040 3050 3060 GEGLHRLGYI LEEIDKKDGD LMYADDTAGW DTRITEDDLQ NEELITEQMA PHHKILAKAI 3070 3080 3090 3100 3110 3120 FKLTYQNKVV KVLRPTPRGA VMDIISRKDQ RGSGQVGTYG LNTFTNMEVQ LIRQMEAEGV 3130 3140 3150 3160 3170 3180 ITQDDMQNPK GLKERVEKWL KECGVDRLKR MAISGDDCVV KPLDERFGTS LLFLNDMGKV 3190 3200 3210 3220 3230 3240 RKDIPQWEPS KGWKNWQEVP FCSHHFHKIF MKDGRSLVVP CRNQDELIGR ARISQGAGWS 3250 3260 3270 3280 3290 3300 LRETACLGKA YAQMWSLMYF HRRDLRLASM AICSAVPTEW FPTSRTTWSI HAHHQWMTTE 3310 3320 3330 3340 3350 3360 DMLKVWNRVW IEDNPNMTDK TPVHSWEDIP YLGKREDLWC GSLIGLSSRA TWAKNIHTAI 3370 3380 3390 TQVRNLIGKE EYVDYMPVMK RYSAPSESEG VL

References

[1]	"Construction and characterization of chimeric tick-borne encephalitis/dengue type 4 viruses." Pletnev A.G., Bray M., Huggins J., Lai C.J. Proc. Natl. Acad. Sci. U.S.A. 89:10532-10536(1992) [PubMed: 1438242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Pletnev A.G. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Structure of dengue virus: implications for flavivirus organization, maturation, and fusion." Kuhn R.J., Zhang W., Rossmann M.G., Pletnev S.V., Corver J., Lenches E., Jones C.T., Mukhopadhyay S., Chipman P.R., Strauss E.G., Baker T.S., Strauss J.H. Cell 108:717-725(2002) [PubMed: 11893341] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (24.00 ANGSTROMS) OF 284-678.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

FJ828987 Other RNA. Translation: ACO82049.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K4R	electron microscopy	24.00	A/B/C	284-678	[»]

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR014001. DEAD-like_helicase.
IPR011492. DEAD_Flavivir.
IPR000069. Env_glycoprot_M_flavivir.
IPR013755. Flav_glyE_cen_dom_subdom1.
IPR013756. Flav_glyE_cen_dom_subdom2.
IPR013754. Flav_glyE_dim.
IPR001122. Flavi_capsidC.
IPR001157. Flavi_NS1.
IPR000752. Flavi_NS2A.
IPR000487. Flavi_NS2B.
IPR000404. Flavi_NS4A.
IPR001528. Flavi_NS4B.
IPR002535. Flavi_propep.
IPR000336. Flv_glyE_Ig-like.
IPR014412. Gen_Poly_FLV.
IPR011999. GlycoprotE_cen/dimer_Flavivir.
IPR011998. GlycoprotE_cen/dimer_vir.
IPR001650. Helicase_C.
IPR014756. Ig_E-set.
IPR009003. Pept_cys/ser_Trypsin-like.
IPR001850. Peptidase_S7.
IPR000208. RNA-dir_pol_flavivirus.
IPR007094. RNA-dir_pol_PSvirus.
IPR002877. rRNA_MeTrfase_RrmJ/FtsJ.
[Graphical view]

Gene3D

G3DSA:3.30.387.10. Flav_glyE_cen_1. 1 hit.
G3DSA:3.30.67.10. Flav_glyE_cen_2. 1 hit.
G3DSA:2.60.98.10. Flav_glyE_dim. 1 hit.
G3DSA:2.60.40.350. Flv_glyE_Ig-like. 1 hit.

Pfam

PF01003. Flavi_capsid. 1 hit.
PF07652. Flavi_DEAD. 1 hit.
PF02832. Flavi_glycop_C. 1 hit.
PF00869. Flavi_glycoprot. 1 hit.
PF01004. Flavi_M. 1 hit.
PF00948. Flavi_NS1. 1 hit.
PF01005. Flavi_NS2A. 1 hit.
PF01002. Flavi_NS2B. 1 hit.
PF01350. Flavi_NS4A. 1 hit.
PF01349. Flavi_NS4B. 1 hit.
PF00972. Flavi_NS5. 1 hit.
PF01570. Flavi_propep. 1 hit.
PF01728. FtsJ. 1 hit.
PF00949. Peptidase_S7. 1 hit.
[Graphical view]

PIRSF

PIRSF003817. Gen_Poly_FLV. 1 hit.

SMART

SM00487. DEXDc. 1 hit.
[Graphical view]

SUPFAM

SSF56983. Flavi_glycoprotE. 1 hit.
SSF81296. Ig_E-set. 1 hit.
SSF50494. Pept_Ser_Cys. 1 hit.

PROSITE

PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

C3V005_9FLAV

Accession

Primary (citable) accession number: C3V005

Entry history

Integrated into UniProtKB/TrEMBL:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)