C3T7B2 (C3T7B2_ECOLX) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Arginine N-succinyltransferase HAMAP-Rule MF_01171
Short name=AST HAMAP-Rule MF_01171
EC=2.3.1.109 HAMAP-Rule MF_01171

Alternative name(s):
AOST HAMAP-Rule MF_01171

Gene names

Name:	astA HAMAP-Rule MF_01171
ORF Names:	ECs2453 EMBL ACI82596.1

Organism

Escherichia coli EMBL ACI82596.1

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of succinyl-CoA to arginine to produce N(2)-succinylarginine By similarity. HAMAP-Rule MF_01171
Catalytic activity	Succinyl-CoA + L-arginine = CoA + N(2)-succinyl-L-arginine. SAAS SAAS007041 HAMAP-Rule MF_01171
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 1/5. SAAS SAAS007041 HAMAP-Rule MF_01171
Sequence similarities	Belongs to the arginine N-succinyltransferase family. HAMAP-Rule MF_01171

Ontologies

Keywords
Biological process	Arginine metabolism SAAS SAAS007041 HAMAP-Rule MF_01171
Molecular function	Acyltransferase SAAS SAAS007041 HAMAP-Rule MF_01171 Transferase
Gene Ontology (GO)
Biological_process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP arginine catabolic process to succinate Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	arginine N-succinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	229	1	Proton donor By similarity HAMAP-Rule MF_01171
	Binding site	125	1	Succinyl-CoA; via amide nitrogen By similarity HAMAP-Rule MF_01171

Sequences

	Sequence		Length	Mass (Da)
	C3T7B2 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 89C7BB412A880BEF	FASTA	344	38,456
	10 20 30 40 50 60 MMVIRPVERS DVSALMQLAS KTGGGLTSLP ANEATLSARI ERAIKTWQGE LPKSEQGYVF 70 80 90 100 110 120 VLEDSETGTV AGICAIEVAV GLNDPWYNYR VGTLVHASKE LNVYNALPTL FLSNDHTGSS 130 140 150 160 170 180 ELCTLFLDPD WRKEGNGYLL SKSRFMFMAA FRDKFNDKVV AEMRGVIDEH GYSPFWQSLG 190 200 210 220 230 240 KRFFSMDFSR ADFLCGTGQK AFIAELMPKH PIYTHFLSQE AQDVIGQVHP QTAPARAVLE 250 260 270 280 290 300 KEGFRYRNYI DIFDGGPTLE CDIDRVRAIR KSRLVEVAEG QPAQGDFPAC LVANENYHHF 310 320 330 340 RVVLVRTDPA TERLILTAAQ LDALKCHAGD RVRLVRLCAE EKTA

References

[1]

"A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis." Leopold S.R., Magrini V., Holt N.J., Shaikh N., Mardis E.R., Cagno J., Ogura Y., Iguchi A., Hayashi T., Mellmann A., Karch H., Besser T.E., Sawyer S.A., Whittam T.S., Tarr P.I. Proc. Natl. Acad. Sci. U.S.A. 106:8713-8718(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 493/89 EMBL ACI82596.1, 86-24 EMBL ACI82597.1, 87-14 EMBL ACI82598.1, TB182A EMBL ACI82599.1 and TW14359 EMBL ACI82600.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU900045 Genomic DNA. Translation: ACI82596.1. EU900046 Genomic DNA. Translation: ACI82597.1. EU900047 Genomic DNA. Translation: ACI82598.1. EU900048 Genomic DNA. Translation: ACI82599.1. EU900049 Genomic DNA. Translation: ACI82600.1.
3D structure databases
SMR	C3T7B2. Positions 1-335.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00185; UER00279.
Family and domain databases
Gene3D	2.40.40.20. 1 hit. 3.40.630.30. 2 hits.
HAMAP	MF_01171. AstA.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR007041. Arg_N-succinylTrfase_Ast/AOST. IPR017650. Arginine_N-succinylTrfase_AstA. IPR009010. Asp_de-COase-like_dom. [Graphical view]
Pfam	PF04958. AstA. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR03243. arg_catab_AOST. 1 hit. TIGR03244. arg_catab_AstA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C3T7B2_ECOLX

Accession

Primary (citable) accession number: C3T7B2

Entry history

Integrated into UniProtKB/TrEMBL:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)