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C3PP60

- SYI_RICAE

UniProt

C3PP60 - SYI_RICAE

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Protein

Isoleucine--tRNA ligase

Gene
ileS, RAF_ORF0859
Organism
Rickettsia africae (strain ESF-5)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei616 – 6161ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRAFR347255:GJCT-807-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:RAF_ORF0859
OrganismiRickettsia africae (strain ESF-5)
Taxonomic identifieri347255 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000002305: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10921092Isoleucine--tRNA ligaseUniRule annotationPRO_1000216256Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi347255.RAF_ORF0859.

Structurei

3D structure databases

ProteinModelPortaliC3PP60.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionUniRule annotationAdd
BLAST
Motifi613 – 6175"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3PP60-1 [UniParc]FASTAAdd to Basket

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MTNTKYYPEV NSNADFAGLE REILKFWQDN NIFQKSIDDR NGELEFIFYD     50
GPPFANGLPH YGHLLTGFIK DVYARYQTIK GKKVERRFGW DCHGLPAEMQ 100
SEKELGISGR LAIANFGIEK FNAHCRASVM KYANDWEEYV TRQARWVNFK 150
NSYKTMDKNF MESVLWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR 200
LDNSYRERAD KAVTVSFVLS HPVATTTGSF KEYRILAWTT TPWTLPSNLA 250
LAVGSDIDYA LVPKNDVCYI IAAYSVSKYA KELGLSGEEN FEIIKGSALQ 300
GLNYKSLFDY FENHPNSFKI FAGDFVVEGD GTGVVHMAPG FGEDDQILCE 350
SKGIELVCPV DNSGKFTKEI PDLEGLQVFD ANDKIIIKLK EQGNWLKTEQ 400
YIHNYPHCWR TDTPLIYKAV PSWYVKVTQF KDRMVELNQQ INWIPFHVKD 450
NLFGKWLENA RDWSISRNRF WGTPLPVWKS DDPKYPRIDV YGSIEELEKD 500
FGVKVTDLHR PFIDELTRPN PDDPTGKSTM RRIEDVFDCW FESGSMPYGQ 550
AHYPFENKEW FEDHFPADFI VEYSAQTRGW FYTLMVLSTA LFDRPPFLNC 600
ICHGIILDST GQKLSKRLNN YADPLELFDK YGSDALRVTM LSSNVVKGQE 650
LLIDKDGKMV FDTLRLFIKP IWNAYHFFTM YANTDSLKGK LNFSSKNVLD 700
VYILSKLKIA VQKIEESLDN FDTQTAYHAV SEFFEVLNNW YIRRSRARFW 750
KSEKDTDKQN AYNTLYSCLD TMAIAMSALV PMISEAIYKG LHHCEERNDT 800
ALSGKSNVIV RKDTSLDKAI SGVSHKIATA LSVPCNDAIS VHLCNYPTLS 850
DFEINHELVA TMDNVLDICS NSLFIRSTEN IRVRQPLASI AIISKHNNNL 900
KDFEDLIKDE INVKAVIYRD DLENYASKKL SINFPMLGKR LPHKMKEIIA 950
ASKKGEWEAI AGGLAICGET LNSDEYKLVL EPYSHIKGAA SFENNSSLLI 1000
LDLELTPELI EEGYARDIVR FIQQARKDAD FSITDRILIE IISEFNLSKI 1050
IDNYGDFIKE QTLGEFAKNF TPDYVSKVEL ENHQIQLKVK KS 1092
Length:1,092
Mass (Da):125,494
Last modified:June 16, 2009 - v1
Checksum:i15ACF54252DACEDC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001612 Genomic DNA. Translation: ACP53720.1.
RefSeqiYP_002845463.1. NC_012633.1.

Genome annotation databases

EnsemblBacteriaiACP53720; ACP53720; RAF_ORF0859.
GeneIDi7814858.
KEGGiraf:RAF_ORF0859.
PATRICi17873887. VBIRicAfr6986_1065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001612 Genomic DNA. Translation: ACP53720.1 .
RefSeqi YP_002845463.1. NC_012633.1.

3D structure databases

ProteinModelPortali C3PP60.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 347255.RAF_ORF0859.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACP53720 ; ACP53720 ; RAF_ORF0859 .
GeneIDi 7814858.
KEGGi raf:RAF_ORF0859.
PATRICi 17873887. VBIRicAfr6986_1065.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RAFR347255:GJCT-807-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of the Rickettsia africae genome reveals that virulence acquisition in Rickettsia species may be explained by genome reduction."
    Fournier P.-E., El Karkouri K., Leroy Q., Robert C., Giumelli B., Renesto P., Socolovschi C., Parola P., Audic S., Raoult D.
    BMC Genomics 10:166-166(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ESF-5.

Entry informationi

Entry nameiSYI_RICAE
AccessioniPrimary (citable) accession number: C3PP60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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