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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Rickettsia africae (strain ESF-5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei605 – 6051ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRAFR347255:GJCT-511-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:RAF_ORF0546
OrganismiRickettsia africae (strain ESF-5)
Taxonomic identifieri347255 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000002305 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Leucine--tRNA ligasePRO_1000202227Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliC3PNE7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi36 – 4611"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3PNE7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQIEQKWQY IWQEEKAFEV SNASSKPKYY VLEMLPYPSG KIHVGHVRNY
60 70 80 90 100
SIGDVIARFM TMQGFNVLHP MGWDAFGLPA EHAAIKNNSH PKKWTYSNIK
110 120 130 140 150
NMKKQLKSMG FSYDWSREIN SCDPEYYKHE QKFFLELYER NLAYQKESFV
160 170 180 190 200
NWDPVDNTVL ANEQVVDGRG WRSGAIVVKR YLKQWFLKIT DYAEELLNEI
210 220 230 240 250
QNLKEWPEAV RSMQEKWIGK SIGANFHFKI KDNEETTIEV FSTKPETIFG
260 270 280 290 300
ASFIGIAFNH PIIERLVSKT PEILAFITQC SHITRSSELE KTEREGVFTG
310 320 330 340 350
LFVIHPFDSN IVLPVIITNF VLMDYGTGAI FGCPAHDECD HELAVKMNLS
360 370 380 390 400
IKQVIKADMD VQKTAYTEDG ILINSDFLNG LTSHEAKQEV IGEFEKLGIG
410 420 430 440 450
KRSVNYRLKD WGISRQRFWG CPIPMIHCEI CGIVPVPYKD LPVILPDDVN
460 470 480 490 500
FDGHGNPLDH HPSWKHVNCP KCDKPAVRET DTFDTFFESS WYFMRYCNSN
510 520 530 540 550
ATEMTDKKAC DYWLPVDKYI GGIEHAVMHL LYARFFTKVM NEQNYVSVRE
560 570 580 590 600
PFKGLFTQGM VLHATYKDEH NNWLYPEEVV KKGNEFFHKE SNNRVVQGRI
610 620 630 640 650
EKMSKSKKNL IDLETMQEQY GADAIRLFVL SDSPPEKDLE WSASGIEGCS
660 670 680 690 700
RFINKLEYMF KAIDSLKDDV NSEVNKELNR LVHFTIKHVA EDIKHFALNR
710 720 730 740 750
AIARMRELSN AISAEISKDK IDVKTVRHGF NVLVQLLNPF IPHITEEIWQ
760 770 780 790 800
KLGNKERLYN LSFPAFDESM LELDTYIMAV QVNGKLRDTY EFKTSVSEDE
810 820 830
IKQVTVSLPK VQKFLEGQEP KKIILVPRKI VNILV
Length:835
Mass (Da):96,747
Last modified:June 16, 2009 - v1
Checksum:i43014F5B9EC39ABE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001612 Genomic DNA. Translation: ACP53457.1.
RefSeqiWP_012719678.1. NC_012633.1.
YP_002845200.1. NC_012633.1.

Genome annotation databases

EnsemblBacteriaiACP53457; ACP53457; RAF_ORF0546.
KEGGiraf:RAF_ORF0546.
PATRICi17873048. VBIRicAfr6986_0659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001612 Genomic DNA. Translation: ACP53457.1.
RefSeqiWP_012719678.1. NC_012633.1.
YP_002845200.1. NC_012633.1.

3D structure databases

ProteinModelPortaliC3PNE7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP53457; ACP53457; RAF_ORF0546.
KEGGiraf:RAF_ORF0546.
PATRICi17873048. VBIRicAfr6986_0659.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciRAFR347255:GJCT-511-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the Rickettsia africae genome reveals that virulence acquisition in Rickettsia species may be explained by genome reduction."
    Fournier P.-E., El Karkouri K., Leroy Q., Robert C., Giumelli B., Renesto P., Socolovschi C., Parola P., Audic S., Raoult D.
    BMC Genomics 10:166-166(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ESF-5.

Entry informationi

Entry nameiSYL_RICAE
AccessioniPrimary (citable) accession number: C3PNE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: June 24, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.