C3PND1 (LSPA_RICAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	RAF_ORF0520

Organism

Rickettsia africae (strain ESF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

347255 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia africae

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP-Rule MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP-Rule MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP-Rule MF_00161
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Lipoprotein signal peptidase HAMAP-Rule MF_00161		PRO_1000203595
Regions
	Transmembrane	73 – 93	21	Helical; Potential
	Transmembrane	97 – 117	21	Helical; Potential
	Transmembrane	135 – 155	21	Helical; Potential
Sites
	Active site	117	1	By similarity
	Active site	144	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	C3PND1 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 00A080A03DA9139D	FASTA	201	23,483
	10 20 30 40 50 60 MLPLLKKLYL TFARSSRIII TLVIIDQLSK WWFIDNLRWK PDLMLKFTSF LNMVYTWNYG 70 80 90 100 110 120 ISFGLMREYY QYSNAIFLIT NTIIVCYLYY LMIRSKTIGS FAGYSFVIGG AVGNLIDRFF 130 140 150 160 170 180 RGAVFDFIHF HYQNYSFPVF NLADCFITIG VIILIEDYYS TKKVIEEKAK GNYDNAQIEA 190 200 MAEKIRNTDK GGNDKIASLQ N

References

[1]

"Analysis of the Rickettsia africae genome reveals that virulence acquisition in Rickettsia species may be explained by genome reduction." Fournier P.-E., El Karkouri K., Leroy Q., Robert C., Giumelli B., Renesto P., Socolovschi C., Parola P., Audic S., Raoult D. BMC Genomics 10:166-166(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ESF-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001612 Genomic DNA. Translation: ACP53441.1.
RefSeq	YP_002845184.1. NC_012633.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	347255.RAF_ORF0520.
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACP53441; ACP53441; RAF_ORF0520.
GeneID	7814944.
KEGG	raf:RAF_ORF0520.
PATRIC	17872982. VBIRicAfr6986_0626.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0597.
HOGENOM	HOG000096992.
KO	K03101.
OMA	ICSKTIG.
ProtClustDB	PRK00376.
Enzyme and pathway databases
BioCyc	RAFR347255:GJCT-492-MONOMER.
UniPathway	UPA00665.
Family and domain databases
HAMAP	MF_00161. LspA.
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_RICAE

Accession

Primary (citable) accession number: C3PND1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program