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Protein

Malate dehydrogenase

Gene

mdh

Organism
Rickettsia africae (strain ESF-5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351NADUniRule annotation
Binding sitei84 – 841SubstrateUniRule annotation
Binding sitei90 – 901SubstrateUniRule annotation
Binding sitei97 – 971NADUniRule annotation
Binding sitei122 – 1221SubstrateUniRule annotation
Binding sitei153 – 1531SubstrateUniRule annotation
Active sitei177 – 1771Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 166NADUniRule annotation
Nucleotide bindingi120 – 1223NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciRAFR347255:GJCT-451-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:RAF_ORF0481
OrganismiRickettsia africae (strain ESF-5)
Taxonomic identifieri347255 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000002305: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Malate dehydrogenasePRO_1000206445Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi347255.RAF_ORF0481.

Structurei

3D structure databases

ProteinModelPortaliC3PN92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiAGNVGEH.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

C3PN92-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQNPKISLI GSGNIGGTLA HLISLRELGD IVLFDVTEGV PQGKALDLMQ
60 70 80 90 100
AGTIAGSDIN IKGTNDYKDI EGSDAIIITA GLPRKPGMSR EDLISINTGI
110 120 130 140 150
MKTVAANVKK YAPDAFVIVI TNPLDVMVYV MLKESGLPHN KVIGMAGVLD
160 170 180 190 200
SSRFNLFLAE EFKVSVSNVN SMVLGGHGDA MVPLARYSTI SGVPIPDLIK
210 220 230 240 250
MGLSSNENIE KIIDRTRNGG GEIVALLKTG SAYYAPAASA IEMLESYLKD
260 270 280 290 300
KRQILTCAAH LQGEYGVHDL YVGVPIMIGK EGVLRVIELQ LTTEEKALFD
310
KSVEGVKKLI ETIK
Length:314
Mass (Da):33,653
Last modified:June 16, 2009 - v1
Checksum:iD3F66A98E3A00418
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001612 Genomic DNA. Translation: ACP53402.1.
RefSeqiYP_002845145.1. NC_012633.1.

Genome annotation databases

EnsemblBacteriaiACP53402; ACP53402; RAF_ORF0481.
GeneIDi7815545.
KEGGiraf:RAF_ORF0481.
PATRICi17872891. VBIRicAfr6986_0583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001612 Genomic DNA. Translation: ACP53402.1.
RefSeqiYP_002845145.1. NC_012633.1.

3D structure databases

ProteinModelPortaliC3PN92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi347255.RAF_ORF0481.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP53402; ACP53402; RAF_ORF0481.
GeneIDi7815545.
KEGGiraf:RAF_ORF0481.
PATRICi17872891. VBIRicAfr6986_0583.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiAGNVGEH.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciRAFR347255:GJCT-451-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the Rickettsia africae genome reveals that virulence acquisition in Rickettsia species may be explained by genome reduction."
    Fournier P.-E., El Karkouri K., Leroy Q., Robert C., Giumelli B., Renesto P., Socolovschi C., Parola P., Audic S., Raoult D.
    BMC Genomics 10:166-166(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ESF-5.

Entry informationi

Entry nameiMDH_RICAE
AccessioniPrimary (citable) accession number: C3PN92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: February 4, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.