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C3PKU4 (SYL_CORA7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:cauri_2469
OrganismCorynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CN-1) (Corynebacterium nigricans) [Complete proteome] [HAMAP]
Taxonomic identifier548476 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length949 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 949949Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199188

Regions

Motif62 – 7312"HIGH" region HAMAP-Rule MF_00049
Motif719 – 7235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C3PKU4 [UniParc].

Last modified June 16, 2009. Version 1.
Checksum: 740B6BD315B1ECE1

FASTA949107,536
        10         20         30         40         50         60 
MTNPSDNTTH RYTPELAAQI ESAWQQYWKD NGTFNAPNPV GPLAEEGKEL PAEKMNIQDM 

        70         80         90        100        110        120 
FPYPSGAGLH VGHPLGYIAT DTYARFNRML GKNVLHTLGY DAFGLPAEQY AIQTGTHPRT 

       130        140        150        160        170        180 
TTEANINNMR RQLGMLGLGH DPRRSVASTD PEFYKWTQWI FLQIYNSWFD EEQQKARPIS 

       190        200        210        220        230        240 
ELIKELEVGK RTTKDGRYYA DLTKEEQRQA LDEFRLVYLS NSTVNWCPGL GTVLANEEVT 

       250        260        270        280        290        300 
AEGKSERGNF PVFRKNLRQW MMRITAYSDR LLDDLELLDW PEKVKSMQRN WIGRSRGAEV 

       310        320        330        340        350        360 
SFHAEGYNID VFTTRPDTLF GAEYVVLAPE HELVDALLSP IPYEDDVDKR WTFGHDDPKE 

       370        380        390        400        410        420 
AVEAYRASIA AKSDLERQEN KEKTGVFLGT YATNPVNGKQ IPIFIADYVL TGYGTGAIMA 

       430        440        450        460        470        480 
VPAHDTRDYE FATVFGLPIT EVVAGGDITK EAYTESGQAV NSANDSLDIN GMSKDEAIAT 

       490        500        510        520        530        540 
IIPWLEEQGV GREKIQYKLR DWLFARQRYW GEPFPIVYDE AGQAYPLPES MLPIELPEVE 

       550        560        570        580        590        600 
DYKPVSFDPD DADSSPQPPL AKAREWVEVE LDLGLGDGPK TYYRDTNVMP QWAGSSWYQL 

       610        620        630        640        650        660 
RYIDPTNDEI FCDLENERYW TGPRTEEHGA NDPGGVDLYV GGVEHAVLHL LYARFWHKVL 

       670        680        690        700        710        720 
FDLGYVSSKE PYRRLYNQGY IQAYAYTDSR GVYVPAAEVE EKDGKFYYNG EEVNQEYGKM 

       730        740        750        760        770        780 
GKSLKNSVAP DDICRDYGAD TLRVYEMSMG PLDTSRPWAT KDVVGAQRFL QRLWRLAIDE 

       790        800        810        820        830        840 
NSGELSTTDA ELSEDDLKHL NRTIAGVRDD YENLRLNTVV AKLIEYVNYL TKAYPKGAPR 

       850        860        870        880        890        900 
AAVEPLAQLV SPVAPHIAEE LWKRFGHEGT ITYEAFPEFD EKYLVDDEIE VPVQINGKVK 

       910        920        930        940 
ARVMVPADAD QATVVGIAKD DERIAELTAG KNVVKEIYVP GRMVNLVVK 

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References

[1]"Complete genome sequence and lifestyle of black-pigmented Corynebacterium aurimucosum ATCC 700975 (formerly C. nigricans CN-1) isolated from a vaginal swab of a woman with spontaneous abortion."
Trost E., Gotker S., Schneider J., Schneiker-Bekel S., Szczepanowski R., Tilker A., Viehoever P., Arnold W., Bekel T., Blom J., Gartemann K.H., Linke B., Goesmann A., Puhler A., Shukla S.K., Tauch A.
BMC Genomics 11:91-91(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700975 / DSM 44827 / CN-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001601 Genomic DNA. Translation: ACP34060.1.
RefSeqYP_002835998.1. NC_012590.1.

3D structure databases

ProteinModelPortalC3PKU4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING169292.cauri_2469.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACP34060; ACP34060; cauri_2469.
GeneID7801606.
KEGGcar:cauri_2469.
PATRIC21481014. VBICorAur68407_2501.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCAUR548476:GH9E-2532-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CORA7
AccessionPrimary (citable) accession number: C3PKU4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 16, 2009
Last modified: April 16, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries