C3PH72 (TRMD_CORA7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	cauri_1583

Organism

Corynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CN-1) (Corynebacterium nigricans) [Complete proteome] [HAMAP]

Taxonomic identifier

548476 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000198566
Regions
	Region	170 – 175	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	146	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	C3PH72 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 23DAA15598D068A3	FASTA	280	31,093
	10 20 30 40 50 60 MRIDVLTIFP EYLDPLRHAL LGKAIEQGKL AVGVHDLRQW ATDAHKSVDD SPYGGGPGMV 70 80 90 100 110 120 MKPEVWGPAL DDVSSGTAST QDLQSALPHL SKPRHDDIHG VEARSYGESE DSDKPLLIVP 130 140 150 160 170 180 TPAGKPFTQA DAQAWSAEEH IVFACGRYEG IDQRVVDDAQ QRYRVREVSI GDYVLIGGEV 190 200 210 220 230 240 AALVIAEAVV RLIPGVLGNR RSHEEDSFSD GLLEGPSYTK PREWRDLQVP DVLTSGDHAR 250 260 270 280 VDRWRREQSL ARTWQRRPEL LDAAELSDAD RAYLETLEED

References

[1]

"Complete genome sequence and lifestyle of black-pigmented Corynebacterium aurimucosum ATCC 700975 (formerly C. nigricans CN-1) isolated from a vaginal swab of a woman with spontaneous abortion." Trost E., Gotker S., Schneider J., Schneiker-Bekel S., Szczepanowski R., Tilker A., Viehoever P., Arnold W., Bekel T., Blom J., Gartemann K.H., Linke B., Goesmann A., Puhler A., Shukla S.K., Tauch A. BMC Genomics 11:91-91(2010) [PubMed: 20137072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700975 / DSM 44827 / CN-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001601 Genomic DNA. Translation: ACP33176.1.
RefSeq	YP_002835114.1. NC_012590.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7800862.
GenomeReviews	Gene locus cauri_1583 in contig CP001601_GR.
KEGG	car:cauri_1583.
PATRIC	21479202. VBICorAur68407_1621.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 2 hits. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_CORA7

Accession

Primary (citable) accession number: C3PH72

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program