C3PH04 (DAPA_CORA7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	cauri_1515

Organism

Corynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CN-1) (Corynebacterium nigricans) [Complete proteome] [HAMAP]

Taxonomic identifier

548476 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000134863
Regions
	Region	60 – 61	2	Pyruvate binding By similarity
Sites
	Active site	173	1	Schiff-base intermediate with substrate By similarity
	Binding site	118	1	Pyruvate By similarity
	Site	145	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	C3PH04 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: F1AC56243B1A964B	FASTA	302	31,165
	10 20 30 40 50 60 MSTGMTAQAG VDTFGRVGVA MVTPFDQDGA LDVSAGRRLA AHLVDNGVDS LILAGTTGES 70 80 90 100 110 120 PTTSLDEKLE LFAAVKEEVG GRAKLCAGAG TNNTATSIEA ARAFADAGAD SLLVVTPYYS 130 140 150 160 170 180 KPSQAGVYAH FTAVADAVDL PVCLYDIPGR SGIPIETETL LRLAEVPNIK AVKDAKGDLV 190 200 210 220 230 240 AAAPLIQETG LAWYSGDDGL NLPWLALGAS GVISVIGHIA PRALADLYVA FDEGDIARAR 250 260 270 280 290 300 EINAKTLSPL VEAQGRLGGV TLVKAALRLQ GIEVGEPRLP VTAADAEEIE ALRHDLEKAG VL

References

[1]

"Complete genome sequence and lifestyle of black-pigmented Corynebacterium aurimucosum ATCC 700975 (formerly C. nigricans CN-1) isolated from a vaginal swab of a woman with spontaneous abortion." Trost E., Gotker S., Schneider J., Schneiker-Bekel S., Szczepanowski R., Tilker A., Viehoever P., Arnold W., Bekel T., Blom J., Gartemann K.H., Linke B., Goesmann A., Puhler A., Shukla S.K., Tauch A. BMC Genomics 11:91-91(2010) [PubMed: 20137072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700975 / DSM 44827 / CN-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001601 Genomic DNA. Translation: ACP33108.1.
RefSeq	YP_002835046.1. NC_012590.1.
3D structure databases
ProteinModelPortal	C3PH04.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7800721.
GenomeReviews	Gene locus cauri_1515 in contig CP001601_GR.
KEGG	car:cauri_1515.
PATRIC	21479062. VBICorAur68407_1551.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HGCISVV.
ProtClustDB	PRK03170.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_CORA7

Accession

Primary (citable) accession number: C3PH04

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program