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Protein

Chorismate synthase

Gene

aroC

Organism
Corynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CN-1) (Corynebacterium nigricans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotationNote: Reduced FMN (FMNH(2)).UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. no protein annotated in this organism
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401NADPUniRule annotation
Binding sitei46 – 461NADPUniRule annotation
Binding sitei305 – 3051FMN; via amide nitrogenUniRule annotation
Binding sitei346 – 3461FMNUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi140 – 1423FMNUniRule annotation
Nucleotide bindingi261 – 2622FMNUniRule annotation
Nucleotide bindingi320 – 3245FMNUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciCAUR548476:GH9E-1419-MONOMER.
UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthaseUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CSUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
Ordered Locus Names:cauri_1388
OrganismiCorynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CN-1) (Corynebacterium nigricans)
Taxonomic identifieri548476 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000002077 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Chorismate synthasePRO_1000132764Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi548476.CaurA7_010100009417.

Structurei

3D structure databases

ProteinModelPortaliC3PGM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG6WDSHT.

Family and domain databases

Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 2 hits.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3PGM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRWTTAGES HGQALVALIE HMPAGVPISQ DDIALQLSRR RLGYGRGARM
60 70 80 90 100
KFEADDLTLL SGIRHGLTLG SPIAIMIGNT EWPKWTTIMS PEALDLEDPE
110 120 130 140 150
VAKAMASGRG APLTRPRPGH ADFAGMVKFD HSEARPILER SSARETAARV
160 170 180 190 200
AAATVARNFL RETLGVEVLS HVISIGASAP YKGPHPSFAD IEAIDASPVR
210 220 230 240 250
ACDKTAEESM ISEIETAKKQ GDTLGGIVEV IVEGLPIGLG SHTSGEDRLD
260 270 280 290 300
AQLAAALMGI QAIKGVEVGD GFAEARRRGS EAHDEMVRTD EGVDRETNRA
310 320 330 340 350
GGLEGGMTNG QTLRLRAAMK PISTVPRALK TVDMSTGGSA TAIHQRSDVC
360 370 380 390 400
AVPAAGVVAE AMVALVLARA VLEKFGGDSL AETKRNIAAY QEYVAGRLEW

GEA
Length:403
Mass (Da):42,653
Last modified:June 16, 2009 - v1
Checksum:iD5DF10F6A53E1DF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001601 Genomic DNA. Translation: ACP32981.1.
RefSeqiWP_012715065.1. NZ_ACLH01000081.1.

Genome annotation databases

EnsemblBacteriaiACP32981; ACP32981; cauri_1388.
KEGGicar:cauri_1388.
PATRICi21478798. VBICorAur68407_1426.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001601 Genomic DNA. Translation: ACP32981.1.
RefSeqiWP_012715065.1. NZ_ACLH01000081.1.

3D structure databases

ProteinModelPortaliC3PGM7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi548476.CaurA7_010100009417.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP32981; ACP32981; cauri_1388.
KEGGicar:cauri_1388.
PATRICi21478798. VBICorAur68407_1426.

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG6WDSHT.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.
BioCyciCAUR548476:GH9E-1419-MONOMER.

Family and domain databases

Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 2 hits.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence and lifestyle of black-pigmented Corynebacterium aurimucosum ATCC 700975 (formerly C. nigricans CN-1) isolated from a vaginal swab of a woman with spontaneous abortion."
    Trost E., Gotker S., Schneider J., Schneiker-Bekel S., Szczepanowski R., Tilker A., Viehoever P., Arnold W., Bekel T., Blom J., Gartemann K.H., Linke B., Goesmann A., Puhler A., Shukla S.K., Tauch A.
    BMC Genomics 11:91-91(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700975 / DSM 44827 / CN-1.

Entry informationi

Entry nameiAROC_CORA7
AccessioniPrimary (citable) accession number: C3PGM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 16, 2009
Last modified: November 11, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.