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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Corynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CN-1) (Corynebacterium nigricans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44ZincUniRule annotation1
Binding sitei59Cysteinyl adenylateUniRule annotation1
Binding sitei228Cysteinyl adenylateUniRule annotation1
Metal bindingi232ZincUniRule annotation1
Metal bindingi257ZincUniRule annotation1
Binding sitei284Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:cauri_1250
OrganismiCorynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CN-1) (Corynebacterium nigricans)
Taxonomic identifieri548476 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004004371 – 414L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseAdd BLAST414

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi548476.CaurA7_010100000447

Structurei

3D structure databases

ProteinModelPortaliC3PG89
SMRiC3PG89
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni44 – 47Cysteinyl adenylate bindingUniRule annotation4
Regioni82 – 84Cysteinyl adenylate bindingUniRule annotation3
Regioni250 – 252Cysteinyl adenylate bindingUniRule annotation3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi46 – 56"HIGH" regionUniRule annotationAdd BLAST11
Motifi188 – 193"ERGGDP" regionUniRule annotation6
Motifi290 – 294"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N Bacteria
COG0215 LUCA
HOGENOMiHOG000245251
KOiK15526
OMAiKVERMAG
OrthoDBiPOG091H004K

Family and domain databases

CDDicd00672 CysRS_core, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_01697 MshC, 1 hit
InterProiView protein in InterPro
IPR024909 Cys-tRNA/MSH_ligase
IPR017812 Mycothiol_ligase_MshC
IPR014729 Rossmann-like_a/b/a_fold
IPR032678 tRNA-synt_1_cat_dom
PANTHERiPTHR10890 PTHR10890, 1 hit
PTHR10890:SF13 PTHR10890:SF13, 1 hit
PfamiView protein in Pfam
PF01406 tRNA-synt_1e, 1 hit
PRINTSiPR00983 TRNASYNTHCYS
TIGRFAMsiTIGR03447 mycothiol_MshC, 1 hit

Sequencei

Sequence statusi: Complete.

C3PG89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSWPVPHID SVPGTPSPLH LYDSADGEVK RLNIKGETAT MYVCGITPYD
60 70 80 90 100
STHLGHAATY LTFDLIYRQL LDNGYKVNYA QNITDVDDPL FERAERDGVD
110 120 130 140 150
WRELGTSQIN LFRSDMELLS VFPPQHFVGA MEAIDEITEM VQALLDAGAA
160 170 180 190 200
YVVDDPDYPD VYASIEATKN FGYESNYSRE QMETFFAERG GDPERPGKRD
210 220 230 240 250
PLDALIWRAH REGEPAWESP FGPGRPGWHI ECSAIATNRL GSSFDIQGGG
260 270 280 290 300
SDLKFPHHEF SAAHAEAALG VERMAQSYVH TGMIGLEGTK MSKSLGNLVF
310 320 330 340 350
VHKLVEAGVD PSAIRLGVFS GHYREDRDWS DEVLHTAQER LERWRAASRN
360 370 380 390 400
PGTVEEIKEV VHNVRLALAE DLDTQRAIAV LDQWAEQALG AAEASEEASD
410
VLRTAVNSLL GVRL
Length:414
Mass (Da):45,942
Last modified:June 16, 2009 - v1
Checksum:iCA66809E7C0AD4D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001601 Genomic DNA Translation: ACP32843.1
RefSeqiWP_010186666.1, NZ_ACLH01000003.1

Genome annotation databases

EnsemblBacteriaiACP32843; ACP32843; cauri_1250
GeneIDi31923873
KEGGicar:cauri_1250

Entry informationi

Entry nameiMSHC_CORA7
AccessioniPrimary (citable) accession number: C3PG89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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