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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Corynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CN-1) (Corynebacterium nigricans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44ZincUniRule annotation1
Binding sitei59Cysteinyl adenylateUniRule annotation1
Binding sitei228Cysteinyl adenylateUniRule annotation1
Metal bindingi232ZincUniRule annotation1
Metal bindingi257ZincUniRule annotation1
Binding sitei284Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:cauri_1250
OrganismiCorynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CN-1) (Corynebacterium nigricans)
Taxonomic identifieri548476 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000002077 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004004371 – 414L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseAdd BLAST414

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi548476.CaurA7_010100000447.

Structurei

3D structure databases

ProteinModelPortaliC3PG89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni44 – 47Cysteinyl adenylate bindingUniRule annotation4
Regioni82 – 84Cysteinyl adenylate bindingUniRule annotation3
Regioni250 – 252Cysteinyl adenylate bindingUniRule annotation3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi46 – 56"HIGH" regionUniRule annotationAdd BLAST11
Motifi188 – 193"ERGGDP" regionUniRule annotation6
Motifi290 – 294"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiSGHDVHY.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

C3PG89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSWPVPHID SVPGTPSPLH LYDSADGEVK RLNIKGETAT MYVCGITPYD
60 70 80 90 100
STHLGHAATY LTFDLIYRQL LDNGYKVNYA QNITDVDDPL FERAERDGVD
110 120 130 140 150
WRELGTSQIN LFRSDMELLS VFPPQHFVGA MEAIDEITEM VQALLDAGAA
160 170 180 190 200
YVVDDPDYPD VYASIEATKN FGYESNYSRE QMETFFAERG GDPERPGKRD
210 220 230 240 250
PLDALIWRAH REGEPAWESP FGPGRPGWHI ECSAIATNRL GSSFDIQGGG
260 270 280 290 300
SDLKFPHHEF SAAHAEAALG VERMAQSYVH TGMIGLEGTK MSKSLGNLVF
310 320 330 340 350
VHKLVEAGVD PSAIRLGVFS GHYREDRDWS DEVLHTAQER LERWRAASRN
360 370 380 390 400
PGTVEEIKEV VHNVRLALAE DLDTQRAIAV LDQWAEQALG AAEASEEASD
410
VLRTAVNSLL GVRL
Length:414
Mass (Da):45,942
Last modified:June 16, 2009 - v1
Checksum:iCA66809E7C0AD4D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001601 Genomic DNA. Translation: ACP32843.1.
RefSeqiWP_010186666.1. NZ_ACLH01000003.1.

Genome annotation databases

EnsemblBacteriaiACP32843; ACP32843; cauri_1250.
KEGGicar:cauri_1250.
PATRICi21478500. VBICorAur68407_1284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001601 Genomic DNA. Translation: ACP32843.1.
RefSeqiWP_010186666.1. NZ_ACLH01000003.1.

3D structure databases

ProteinModelPortaliC3PG89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi548476.CaurA7_010100000447.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP32843; ACP32843; cauri_1250.
KEGGicar:cauri_1250.
PATRICi21478500. VBICorAur68407_1284.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiSGHDVHY.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHC_CORA7
AccessioniPrimary (citable) accession number: C3PG89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.