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C3PBM5

- C3PBM5_BACAA

UniProt

C3PBM5 - C3PBM5_BACAA

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Protein

N5-carboxyaminoimidazole ribonucleotide synthase

Gene

purK

Organism
Bacillus anthracis (strain A0248)
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761ATP; via carbonyl oxygenImported
Metal bindingi104 – 1041Magnesium 1; via carbonyl oxygenImported
Binding sitei107 – 1071ADPImported
Binding sitei107 – 1071ATPImported
Metal bindingi110 – 1101Magnesium 1Imported
Binding sitei147 – 1471ADPImported
Binding sitei147 – 1471ATPImported
Binding sitei190 – 1901ADPImported
Binding sitei190 – 1901ATPImported
Metal bindingi255 – 2551CalciumImported
Metal bindingi255 – 2551Magnesium 2Imported
Binding sitei255 – 2551ATPImported
Metal bindingi268 – 2681CalciumImported
Metal bindingi268 – 2681Magnesium 2Imported
Metal bindingi269 – 2691Magnesium 1; via carbonyl oxygenImported
Binding sitei340 – 3401ATPImported

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi153 – 1586ATPImported
Nucleotide bindingi154 – 1585ADPImported
Nucleotide bindingi182 – 1854ADPImported
Nucleotide bindingi182 – 1854ATPImported
Nucleotide bindingi213 – 2164ADPImported
Nucleotide bindingi213 – 2164ATPImported
Nucleotide bindingi255 – 2573ADPImported
Nucleotide bindingi267 – 2682ADPImported
Nucleotide bindingi267 – 2682ATPImported
Nucleotide bindingi272 – 2754ATPImported
Nucleotide bindingi347 – 3482ATPImported

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NAD binding Source: InterPro
  4. phosphoribosylaminoimidazole carboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: InterPro
  2. oxidation-reduction process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation, LyaseImported

Keywords - Biological processi

Purine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, CalciumImported, MagnesiumImported, Metal-bindingImported, Nucleotide-binding

Enzyme and pathway databases

BioCyciBANT592021:GJAQ-334-MONOMER.
UniPathwayiUPA00074; UER00942.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)
Short name:
N5-CAIR synthaseUniRule annotation
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation
Gene namesi
Name:purKImported
Ordered Locus Names:BAA_0344Imported
OrganismiBacillus anthracis (strain A0248)Imported
Taxonomic identifieri592021 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000002335: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi592021.BAA_0344.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3Q2OX-ray1.96A/B1-383[»]
3QFFX-ray1.96A/B1-383[»]
3R5HX-ray2.20A/B1-383[»]
3V4SX-ray2.02A/B1-378[»]
4DLKX-ray2.02A/B1-380[»]
ProteinModelPortaliC3PBM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotation
Contains ATP-grasp domain.SAAS annotation

Phylogenomic databases

eggNOGiCOG0026.
HOGENOMiHOG000034029.
KOiK01589.
OMAiDCILEGW.
OrthoDBiEOG6QZMX7.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR005875. AIR_COase_ATPase-su.
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006140. D-isomer_DH_NAD-bd.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02826. 2-Hacid_dh_C. 1 hit.
PF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3PBM5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRIILPGKT IGIIGGGQLG RMMALAAKEM GYKIAVLDPT KNSPCAQVAD
60 70 80 90 100
IEIVASYDDL KAIQHLAEIS DVVTYEFENI DYRCLQWLEK HAYLPQGSQL
110 120 130 140 150
LSKTQNRFTE KNAIEKAGLP VATYRLVQNQ EQLTEAIAEL SYPSVLKTTT
160 170 180 190 200
GGYDGKGQVV LRSEADVDEA RKLANAAECI LEKWVPFEKE VSVIVIRSVS
210 220 230 240 250
GETKVFPVAE NIHVNNILHE SIVPARITEE LSQKAIAYAK VLADELELVG
260 270 280 290 300
TLAVEMFATA DGEIYINELA PRPHNSGHYT QDACETSQFG QHIRAICNLP
310 320 330 340 350
LGETNLLKPV VMVNILGEHI EGVLRQVNRL TGCYLHLYGK EEAKAQRKMG
360 370 380
HVNILNDNIE VALEKAKSLH IWDHQEQLLE GKR
Length:383
Mass (Da):42,517
Last modified:June 16, 2009 - v1
Checksum:iBE0E5E1A56F88287
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001598 Genomic DNA. Translation: ACQ48995.1.
RefSeqiYP_002864907.1. NC_012659.1.

Genome annotation databases

EnsemblBacteriaiACQ48995; ACQ48995; BAA_0344.
GeneIDi7848383.
KEGGibai:BAA_0344.
PATRICi18766429. VBIBacAnt132916_0589.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001598 Genomic DNA. Translation: ACQ48995.1 .
RefSeqi YP_002864907.1. NC_012659.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3Q2O X-ray 1.96 A/B 1-383 [» ]
3QFF X-ray 1.96 A/B 1-383 [» ]
3R5H X-ray 2.20 A/B 1-383 [» ]
3V4S X-ray 2.02 A/B 1-378 [» ]
4DLK X-ray 2.02 A/B 1-380 [» ]
ProteinModelPortali C3PBM5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 592021.BAA_0344.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACQ48995 ; ACQ48995 ; BAA_0344 .
GeneIDi 7848383.
KEGGi bai:BAA_0344.
PATRICi 18766429. VBIBacAnt132916_0589.

Phylogenomic databases

eggNOGi COG0026.
HOGENOMi HOG000034029.
KOi K01589.
OMAi DCILEGW.
OrthoDBi EOG6QZMX7.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00942 .
BioCyci BANT592021:GJAQ-334-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProi IPR005875. AIR_COase_ATPase-su.
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006140. D-isomer_DH_NAD-bd.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF02826. 2-Hacid_dh_C. 1 hit.
PF02222. ATP-grasp. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01161. purK. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of Bacillus anthracis A0248."
    Dodson R.J., Munk A.C., Bruce D., Detter C., Tapia R., Sutton G., Sims D., Brettin T.
    Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A0248Imported.
  2. "Structure of N5-carboxyaminoimidazole ribonucleotide synthase (PurK) from Bacillus anthracis."
    Tuntland M.L., Johnson M.E., Fung L.W., Santarsiero B.D.
    Acta Crystallogr. D 67:870-874(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.
  3. "Crystal Structure of purK: N5-carboxyaminoimidazole ribonucleotide synthetase."
    Fung L.W., Tuntland M.L., Santarsiero B.D., Johnson M.E.
    Submitted (JAN-2011) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) IN COMPLEX WITH ADP.
  4. "Crystal Structure of purK: N5-carboxyaminoimidazole ribonucleotide synthetase."
    Fung L.W., Tuntland M.L., Santarsiero B.D., Johnson M.E.
    Submitted (MAR-2011) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH ADP.
  5. "Crystal Structure of ADP-ATP complex of purK: N5-carboxyaminoimidazole ribonucleotide synthetase."
    Fung L.W., Tuntland M.L., Santarsiero B.D., Johnson M.E.
    Submitted (DEC-2011) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 1-378 IN COMPLEX WITH ADP; ATP AND MAGNESIUM.
  6. "Crystal Structure of purK: N5-carboxyaminoimidazole ribonucleotide synthetase."
    Fung L.W., Tuntland M.L., Santarsiero B.D., Johnson M.E.
    Submitted (FEB-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 1-380 IN COMPLEX WITH ATP AND CALCIUM.

Entry informationi

Entry nameiC3PBM5_BACAA
AccessioniPrimary (citable) accession number: C3PBM5
Entry historyi
Integrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteomeImported

External Data

Dasty 3