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Protein

Beta-galactosidase

Gene

lacZ

Organism
Vibrio cholerae serotype O1 (strain MJ-1236)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201SubstrateUniRule annotation
Metal bindingi218 – 2181SodiumUniRule annotation
Binding sitei218 – 2181SubstrateUniRule annotation
Sitei375 – 3751Transition state stabilizerUniRule annotation
Sitei409 – 4091Transition state stabilizerUniRule annotation
Metal bindingi434 – 4341Magnesium 1UniRule annotation
Metal bindingi436 – 4361Magnesium 1UniRule annotation
Active sitei479 – 4791Proton donorUniRule annotation
Metal bindingi479 – 4791Magnesium 1UniRule annotation
Binding sitei479 – 4791SubstrateUniRule annotation
Active sitei555 – 5551NucleophileUniRule annotation
Metal bindingi615 – 6151Magnesium 2UniRule annotation
Metal bindingi619 – 6191Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi622 – 6221SodiumUniRule annotation
Binding sitei622 – 6221SubstrateUniRule annotation
Binding sitei1015 – 10151SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationSAAS annotation, Hydrolase

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation, SodiumUniRule annotation

Enzyme and pathway databases

BioCyciVCHO593588:GI2R-1063-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotationSAAS annotation (EC:3.2.1.23UniRule annotationSAAS annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:VCD_002010Imported
OrganismiVibrio cholerae serotype O1 (strain MJ-1236)Imported
Taxonomic identifieri593588 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000001618 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi593588.VCD_002010.

Structurei

3D structure databases

ProteinModelPortaliC3NVQ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni555 – 5584Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiTNRHEHH.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3NVQ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYAVEQRRYW LVASRIQEDL MRNFSDILLS QDWQNPHIVK WHCRTPHVPL
60 70 80 90 100
HSYRTEQEAR LDVGGNRQSL NGQWRFALFE KPEAVEPAVI DPDFDDSAWA
110 120 130 140 150
HIPVPSNWQM QGFDKPIYTN IQYPFADRPP YVPQDNPTGC YRHRFTLEKQ
160 170 180 190 200
ALTESIRIVF DGVNSAFHLW CNGHWVGYSQ DSRLPAEFEL TPYLQEGENL
210 220 230 240 250
LVAMVLRWSD GSYLEDQDMW WLSGIFRDVY LYRKPILAIE DFFIRTELDA
260 270 280 290 300
LYQHAELRVE TRLSQVTRHH QVQVALFDAQ GECVARSQAL HTGQRVVDEK
310 320 330 340 350
GAWHDKTEHS LAICSPTLWS DEAPYLYRCV ICLLDEDGAP IEFESAAVGF
360 370 380 390 400
RKVEITQGLL KLNGQPLLIR GVNRHEHHPE LGHVMDEASM RRDIELMKQH
410 420 430 440 450
NFNAVRTAHY PNHPRWYELC DEYGLYVVDE ANLETHGQFP MSRLSNDPQW
460 470 480 490 500
VNAYLQRMIG MVERDKNHPC VIIWSLGNES GIGTNHHAMY QWTKQRDPSR
510 520 530 540 550
PVQYEGGGAN TAATDIVCPM YARVDQHQPH PAVPKYALKN WISLPQENRP
560 570 580 590 600
LILCEYAHAM GNSLGAFYKY WQAFREFPRL QGGFIWDWVD QGISKWDSEG
610 620 630 640 650
RHYWGYGGDF GDTINDRQFC INGLLFPDRT PHPALHEVKK VQQPYQFSLS
660 670 680 690 700
YPKLTIHNER LFAALPLELV VSVLCDGQEI KQERLPLDIA PRGTITLDLA
710 720 730 740 750
SLPMLPEHEY HLNAVLLCRE DQPWSNAGHC IASEQWCLQP RRSMLPKITH
760 770 780 790 800
APLPQWQQDG DKVRIEAANQ QWQFNRQTGL LEQWWQNGQP VLSEPLRDNF
810 820 830 840 850
YRAVLDNDIG TSEAQHLDPN SWIARWHAAG LDKLRVECDD LRVTTLNESV
860 870 880 890 900
EVVIDVAHYH QQALALRTRW RYQIFGDARV ELNVEVMLCS DLPPLPRVGL
910 920 930 940 950
TLALPVAENP VSWFGRGPHE NYPDRLQSAH VGRYTATVDE LHTPYIFPSE
960 970 980 990 1000
NGLRCDTRQL QVGALVVEGH FHFSLSRYSQ TMLDKAKHSN ELVAGDKWYL
1010 1020 1030 1040
NLDAQHMGVG GDDSWSQSVH PEFLLTQPHY QYQLTLRVKA SSPQ
Length:1,044
Mass (Da):120,685
Last modified:June 15, 2009 - v1
Checksum:i2AF5D57EC04B6908
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001485 Genomic DNA. Translation: ACQ60179.1.
RefSeqiYP_002877749.1. NC_012668.1.

Genome annotation databases

EnsemblBacteriaiACQ60179; ACQ60179; VCD_002010.
GeneIDi7855259.
KEGGivcj:VCD_002010.
PATRICi20075382. VBIVibCho20143_2033.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001485 Genomic DNA. Translation: ACQ60179.1.
RefSeqiYP_002877749.1. NC_012668.1.

3D structure databases

ProteinModelPortaliC3NVQ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi593588.VCD_002010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACQ60179; ACQ60179; VCD_002010.
GeneIDi7855259.
KEGGivcj:VCD_002010.
PATRICi20075382. VBIVibCho20143_2033.

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiTNRHEHH.
OrthoDBiEOG6XWV0T.

Enzyme and pathway databases

BioCyciVCHO593588:GI2R-1063-MONOMER.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MJ-1236Imported.

Entry informationi

Entry nameiC3NVQ6_VIBCJ
AccessioniPrimary (citable) accession number: C3NVQ6
Entry historyi
Integrated into UniProtKB/TrEMBL: June 15, 2009
Last sequence update: June 15, 2009
Last modified: March 3, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.