C3NUN6 (C3NUN6_VIBCJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase B HAMAP-Rule MF_01080
EC=5.4.99.25 HAMAP-Rule MF_01080

Alternative name(s):
tRNA pseudouridine(55) synthase HAMAP-Rule MF_01080
tRNA pseudouridylate synthase HAMAP-Rule MF_01080
tRNA-uridine isomerase HAMAP-Rule MF_01080

Gene names

Name:	truB HAMAP-Rule MF_01080
Ordered Locus Names:	VCD_003766

Organism

Vibrio cholerae serotype O1 (strain MJ-1236) [Complete proteome] [HAMAP] EMBL ACQ61922.1

Taxonomic identifier

593588 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01080 SAAS SAAS014780
Catalytic activity	tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01080 SAAS SAAS014780
Sequence similarities	Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. HAMAP-Rule MF_01080

Ontologies

Keywords
Biological process	tRNA processing HAMAP-Rule MF_01080 SAAS SAAS014780
Molecular function	Isomerase HAMAP-Rule MF_01080 SAAS SAAS014780 Lyase EMBL ACQ61922.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro lyase activity Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	47	1	Nucleophile By similarity HAMAP-Rule MF_01080

Sequences

	Sequence		Length	Mass (Da)
	C3NUN6 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: C568441464501A53	FASTA	312	34,488
	10 20 30 40 50 60 MARRRKGRVI HGVILLDKPT GISSNDALQK VKRLYGAEKA GHTGALDPLA TGMLPICLGE 70 80 90 100 110 120 ATKFSQFLLD SDKRYRVIAK LGERTDTSDS DGQVVQTRPV HVDYDTLLAC IAKFRGETDQ 130 140 150 160 170 180 VPSMFSALKY QGRPLYEYAR QGIEVPREAR KITVYEIELH RFEGDEVEME VHCSKGTYIR 190 200 210 220 230 240 TIVDDLGEML GCGAHVTMLR RVGVANYPYE RMVTLEQLNA LVEQAHRDEK AVADVLDPLL 250 260 270 280 290 300 LPMDTAVEAL PEVNVIPELM TLIQHGQAVQ VSGAPSDGMV RITGGEQKLF LGVGEIDDNG 310 KVAPKRLVVY GE

References

[1]

"Comparative genomics reveals mechanism for short-term and long-term clonal transitions in pandemic Vibrio cholerae." Chun J., Grim C.J., Hasan N.A., Lee J.H., Choi S.Y., Haley B.J., Taviani E., Jeon Y.-S., Kim D.W., Lee J.-H., Brettin T.S., Bruce D.C., Challacombe J.F., Detter J.C., Han C.S., Munk A.C., Chertkov O., Meincke L. , Saunders E., Walters R.A., Huq A., Nair G.B., Colwell R.R. Proc. Natl. Acad. Sci. U.S.A. 106:15442-15447(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MJ-1236.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001485 Genomic DNA. Translation: ACQ61922.1.
RefSeq	YP_002879492.1. NC_012668.1.
3D structure databases
ProteinModelPortal	C3NUN6.
SMR	C3NUN6. Positions 10-309.
ModBase	Search...
Protein-protein interaction databases
STRING	593588.VCD_003766.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACQ61922; ACQ61922; VCD_003766.
GeneID	7856195.
KEGG	vcj:VCD_003766.
PATRIC	20078947. VBIVibCho20143_3776.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0130.
HOGENOM	HOG000231223.
KO	K03177.
OMA	LGCGAYV.
ProtClustDB	PRK05033.
Family and domain databases
HAMAP	MF_01080. TruB_bact.
InterPro	IPR002501. PsdUridine_synth. IPR020103. PsdUridine_synth_cat_dom. IPR015947. PUA-like_domain. IPR014780. tRNA_psdUridine_synth_TruB. IPR015240. tRNA_sdUridine_synth_fam1_C. [Graphical view]
PANTHER	PTHR13767. PTHR13767. 1 hit.
Pfam	PF09157. TruB-C_2. 1 hit. PF01509. TruB_N. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR00431. TruB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C3NUN6_VIBCJ

Accession

Primary (citable) accession number: C3NUN6

Entry history

Integrated into UniProtKB/TrEMBL:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)