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Protein

CCA-adding enzyme

Gene

cca

Organism
Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41ATP or CTPUniRule annotation1
Binding sitei44ATP or CTPUniRule annotation1
Metal bindingi53MagnesiumUniRule annotation1
Metal bindingi55MagnesiumUniRule annotation1
Metal bindingi106MagnesiumUniRule annotation1
Binding sitei129ATP or CTPUniRule annotation1
Binding sitei149ATP or CTPUniRule annotation1
Binding sitei158ATP or CTPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

RNA repair, tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotation
Ordered Locus Names:YN1551_1673
OrganismiSulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)
Taxonomic identifieri419942 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000006818 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002141441 – 412CCA-adding enzymeAdd BLAST412

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000226400.
KOiK07558.
OMAiKGTWLKG.

Family and domain databases

HAMAPiMF_01264. CCA_arch. 1 hit.
InterProiIPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.

Sequencei

Sequence statusi: Complete.

C3NHZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEEEVLKII KPTEEDKKGI EKVLEIIRER LNKLDFEVEG SFRKGTWLRQ
60 70 80 90 100
DTDIDVFVFY PKDVGKEYLE RNALNDIINR IKDLDYTLAY AEHPYVIVNI
110 120 130 140 150
NNVEVDIVPA LRVESGDKAI TAVDRTPFHT KYVTSHLDER GKDEVRLLKR
160 170 180 190 200
FMKGIGVYGA ELKVQGFSGY ATELLIIYYG NFRKVLEEAS KWKHPIKIEL
210 220 230 240 250
TKPMKIFSEP LIIPDPVDPK RNVTAAVSLK NIATFSIAAK YYLKNPSIEF
260 270 280 290 300
FFPSKKVEEK VKGDVLILRL NLDEKSSEDI VWGQIKRSVN KIERALKQYG
310 320 330 340 350
FRVIDVQAWG DTNNITIAVQ LESKNIGQYY LNIGPQYYSE TIEDFIQKND
360 370 380 390 400
NIWVGEDGRL YSIKERKEYD AETIAKKNIV LKVKYNIESY WLQNKEDQQI
410
MKFLRKTPTW LK
Length:412
Mass (Da):48,050
Last modified:June 16, 2009 - v1
Checksum:i13640523B9E2991B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001404 Genomic DNA. Translation: ACP48755.1.
RefSeqiWP_012717536.1. NC_012623.1.

Genome annotation databases

EnsemblBacteriaiACP48755; ACP48755; YN1551_1673.
GeneIDi7810631.
KEGGisin:YN1551_1673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001404 Genomic DNA. Translation: ACP48755.1.
RefSeqiWP_012717536.1. NC_012623.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP48755; ACP48755; YN1551_1673.
GeneIDi7810631.
KEGGisin:YN1551_1673.

Phylogenomic databases

HOGENOMiHOG000226400.
KOiK07558.
OMAiKGTWLKG.

Family and domain databases

HAMAPiMF_01264. CCA_arch. 1 hit.
InterProiIPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCCA_SULIN
AccessioniPrimary (citable) accession number: C3NHZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.