C3NH14 (PURL_SULIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	YN1551_1329

Organism

Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2) [Complete proteome] [HAMAP]

Taxonomic identifier

419942 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	709 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 709	709	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000206048
Regions
	Nucleotide binding	95 – 106	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	C3NH14 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 9C41095D1EB3F232	FASTA	709	77,165
	10 20 30 40 50 60 MGLNLLPIEM DDIRKRLDRE PNEIEWRVID AVWSEHCSYK SSKIFLKSFS IDSPNVIMGI 70 80 90 100 110 120 KDWQDAGAVD IGDGWAIVIK VESHNHPSAI DPFNGAATGV GGIIRDIISK GAKPIALMDM 130 140 150 160 170 180 IRVGNLKIRK NVWLLKNIIA GIAAYGNSIG VPVVGGELSF DDTYNDNPLV DVAAIGIVRK 190 200 210 220 230 240 DKIKPSIVDK AGLKLVLAGL TGIDGLGGAS FASRKLSGED EIGAVQIADP FAGKIILDVT 250 260 270 280 290 300 LEIADKVEAI KDLGGGGLAV AVTEMANGLG AIVDIEKIPL RVKNMDPADV IISETQERML 310 320 330 340 350 360 YAVEEKNVEE VCKAFEEYEY PCSVIGEITS EPIIKFRYFG KDLVSLPTNA LLEPPKFLWP 370 380 390 400 410 420 IKNVRKNVEE KNVDLSLEST IYTVLSHPDL VSKEWVYSQF DYEVNTSTVV KPGDANGAVV 430 440 450 460 470 480 SLPNGKLLAI KADGNPDLCS EDAYECGKGI VAEAYRNLAT VGARGMVAVD HLQFGDPKKP 490 500 510 520 530 540 EVYYTFVEAI RGIGEATRFF NIPIVGGKVS FYNENSQGKP IKPTPLIVMA GLVQGKLLKN 550 560 570 580 590 600 RVEDSSYVVL LGYTRKELGG SLLSKIFKVP SQAPKVRLQE DLLSSEVVID AINEEKITFA 610 620 630 640 650 660 KDISRGGLAA SLFNIIVHGY GVEISTKSIL SDTDNVVENL FSESSGRFVI LTNEPEWIVE 670 680 690 700 KSRSKGIVAS IIGKVNKKTS ILTIDNTDYD LKTIVNNYFN FLEEVIGNG

References

[1]	"Biogeography of the Sulfolobus islandicus pan-genome." Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J. Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610(2009) [PubMed: 19435847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Y.N.15.51 / Yellowstone #2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001404 Genomic DNA. Translation: ACP48424.1.
RefSeq	YP_002840346.1. NC_012623.1.
3D structure databases
ProteinModelPortal	C3NH14.
ModBase	Search...
Protein-protein interaction databases
STRING	C3NH14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7810114.
GenomeReviews	Gene locus YN1551_1329 in contig CP001404_GR.
KEGG	sin:YN1551_1329.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_SULIN

Accession

Primary (citable) accession number: C3NH14

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program