C3N969 (RTCA_SULIY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	YG5714_0238

Organism

Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1) [Complete proteome] [HAMAP]

Taxonomic identifier

439386 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_1000204097
Regions
	Nucleotide binding	282 – 285	4	ATP By similarity
Sites
	Active site	306	1	Tele-AMP-histidine intermediate By similarity
	Binding site	101	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	C3N969 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: D5196BC3FC6CAA71	FASTA	337	36,685
	10 20 30 40 50 60 MIEIDGSFGE GGGQILRTSL TLSVITGKPF RIFNIRANRP NPGLQRQHLW AVKAMKMISN 70 80 90 100 110 120 AETKGDEVGS KELIFVPHEI KGNTNIDIDV GTAGSVTLII QTVLPAIINK NVRIRIKGGT 130 140 150 160 170 180 DVPKSPTIDY IRLVYLEILR KIGIEAKLNL IKRGHYPEGG GEVIIENVNG NPSAFSLLEL 190 200 210 220 230 240 GKLTIIKGIS HVSSLPAHIA ERQMNSAREI LSKLGVPIEI ETDVRQGEVS KGSGIALAAI 250 260 270 280 290 300 GEKSIIGADS LGERGKRAEI VGEEAARILI NNLNTKASVD IHMSDMLMIF ASLYGGEYIG 310 320 330 AELTSHAYTN MEIIKKFLDI KIDVSGKRPF RFKAKIF

References

[1]	"Biogeography of the Sulfolobus islandicus pan-genome." Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J. Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610(2009) [PubMed: 19435847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Y.G.57.14 / Yellowstone #1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001403 Genomic DNA. Translation: ACP44531.1.
RefSeq	YP_002836453.1. NC_012622.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C3N969.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7806388.
GenomeReviews	Gene locus YG5714_0238 in contig CP001403_GR.
KEGG	siy:YG5714_0238.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GGTDVAW.
ProtClustDB	PRK04204.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_SULIY

Accession

Primary (citable) accession number: C3N969

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	June 16, 2009
Last modified:	December 14, 2011
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program