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Protein

[LysW]-lysine/[LysW]-ornithine hydrolase

Gene

lysK

Organism
Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the release of L-lysine from [LysW]-gamma-L-lysine and the release of L-ornithine from [LysW]-L-ornithine.UniRule annotation

Catalytic activityi

[LysW]-C-terminal-L-glutamyl-gamma-L-lysine + H2O = [LysW]-C-terminal-L-glutamate + L-lysine.UniRule annotation
[LysW]-L-ornithine + H2O = [LysW] + L-ornithine.UniRule annotation

Cofactori

Zn2+UniRule annotation, Co2+UniRule annotationNote: Binds 2 Zn2+ or Co2+ ions per subunit.UniRule annotation

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 5 of the subpathway that synthesizes L-lysine from L-alpha-aminoadipate (Thermus route).UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. [LysW]-aminoadipate/[LysW]-glutamate kinase (lysZ)
  3. N-acetyl-gamma-glutamyl-phosphate/N-acetyl-gamma-aminoadipyl-phosphate reductase (argC)
  4. Acetylornithine/acetyl-lysine aminotransferase (argD), Acetylornithine/acetyl-lysine aminotransferase (argD)
  5. [LysW]-lysine/[LysW]-ornithine hydrolase (lysK)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-lysine from L-alpha-aminoadipate (Thermus route), the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

Pathwayi: L-arginine biosynthesis

This protein is involved in the pathway L-arginine biosynthesis, which is part of Amino-acid biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68Cobalt or zinc 1UniRule annotation1
Active sitei70UniRule annotation1
Metal bindingi92Cobalt or zinc 1UniRule annotation1
Metal bindingi92Cobalt or zinc 2UniRule annotation1
Active sitei122Proton acceptorUniRule annotation1
Metal bindingi123Cobalt or zinc 2UniRule annotation1
Metal bindingi146Cobalt or zinc 1UniRule annotation1
Metal bindingi317Cobalt or zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Lysine biosynthesis
LigandCobalt, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00033; UER00039.
UPA00068.

Names & Taxonomyi

Protein namesi
Recommended name:
[LysW]-lysine/[LysW]-ornithine hydrolaseUniRule annotation (EC:3.5.1.-UniRule annotation)
Gene namesi
Name:lysKUniRule annotation
Ordered Locus Names:YG5714_2101
OrganismiSulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1)
Taxonomic identifieri439386 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000002308 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002136181 – 346[LysW]-lysine/[LysW]-ornithine hydrolaseAdd BLAST346

Structurei

3D structure databases

ProteinModelPortaliC3N866.
SMRiC3N866.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family. LysK subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000105331.
KOiK05831.
OMAiNGEPSGW.
OrthoDBiPOG094A00MT.

Family and domain databases

CDDicd05653. M20_ArgE_LysK. 1 hit.
Gene3Di3.30.70.360. 1 hit.
HAMAPiMF_01120. LysK. 1 hit.
InterProiView protein in InterPro
IPR001261. ArgE/DapE_CS.
IPR010175. DapE_lys_deAc.
IPR023556. LysK.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
PfamiView protein in Pfam
PF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01902. dapE-lys-deAc. 1 hit.
PROSITEiView protein in PROSITE
PS00758. ARGE_DAPE_CPG2_1. 1 hit.

Sequencei

Sequence statusi: Complete.

C3N866-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQEKELVKQ KAKELLLDLL SIYTPSKNET NATKFFEKIS NEFNLKLEIL
60 70 80 90 100
PDSNSFILGE GEILLASHVD TVPGYIEPKI ENEVIYGRGA VDAKGPLISM
110 120 130 140 150
IIAAWLLNEK GIKVMVSGLA DEESTSIGAK ELTLKNFNFK HIIVGEPSNG
160 170 180 190 200
TDIVVEYRGS IQLDIMCEST PEHSSSAKSN LIVDISKKII EVYKQPENYD
210 220 230 240 250
KPSIVPTIIR AGESYNVTPA KLYLHFDVRY AINNKRDDLI NEIKDKFQEC
260 270 280 290 300
GLKIVDETPP VKVSINNPVV KSLTRALLKQ NIKPRLVRKA GTSDMNILQK
310 320 330 340
ITTSIATYGP GNSMLEHTNQ EKITLDEIYI GVKTYMLAIE ELWQKS
Length:346
Mass (Da):38,769
Last modified:June 16, 2009 - v1
Checksum:iBDE8A5BEEBA0BAF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001403 Genomic DNA. Translation: ACP46353.1.
RefSeqiWP_012711946.1. NC_012622.1.

Genome annotation databases

EnsemblBacteriaiACP46353; ACP46353; YG5714_2101.
GeneIDi7807920.
KEGGisiy:YG5714_2101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001403 Genomic DNA. Translation: ACP46353.1.
RefSeqiWP_012711946.1. NC_012622.1.

3D structure databases

ProteinModelPortaliC3N866.
SMRiC3N866.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP46353; ACP46353; YG5714_2101.
GeneIDi7807920.
KEGGisiy:YG5714_2101.

Phylogenomic databases

HOGENOMiHOG000105331.
KOiK05831.
OMAiNGEPSGW.
OrthoDBiPOG094A00MT.

Enzyme and pathway databases

UniPathwayiUPA00033; UER00039.
UPA00068.

Family and domain databases

CDDicd05653. M20_ArgE_LysK. 1 hit.
Gene3Di3.30.70.360. 1 hit.
HAMAPiMF_01120. LysK. 1 hit.
InterProiView protein in InterPro
IPR001261. ArgE/DapE_CS.
IPR010175. DapE_lys_deAc.
IPR023556. LysK.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
PfamiView protein in Pfam
PF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01902. dapE-lys-deAc. 1 hit.
PROSITEiView protein in PROSITE
PS00758. ARGE_DAPE_CPG2_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLYSK_SULIY
AccessioniPrimary (citable) accession number: C3N866
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: February 15, 2017
This is version 49 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.