C3N7E3 (SYA_SULIY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	YG5714_1881

Organism

Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1) [Complete proteome] [HAMAP]

Taxonomic identifier

439386 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	900 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 900	900	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_1000202051
Sites
	Metal binding	604	1	Zinc By similarity
	Metal binding	608	1	Zinc By similarity
	Metal binding	708	1	Zinc By similarity
	Metal binding	712	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	C3N7E3 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: AE89FEAD497CB69C	FASTA	900	103,090
	10 20 30 40 50 60 MKASEEEYRL NFFIKNDFKR KICKSCKTPF WTRDEKKEYC SDIPCTDYYF FDINIKSQPL 70 80 90 100 110 120 TVKEAREKFL SFFEKRGHTR ISPKPVLARW REDLYLTIAS IVDFQPHVTS GLVPPPANPL 130 140 150 160 170 180 VVSQPSIRLE DIDNVGITFG RHLTTFEMAA HHAFNYPDHY VYWKEETTAY ATEFFTKELG 190 200 210 220 230 240 IPEEELNFKE SWWEGGGNAG PCLEVTVGGL ELATLVFMQY KITDNGNYTP LKLKIVDTGY 250 260 270 280 290 300 GVERIAWITQ KTPSAFHAIY GNLVYKFFNK IGVAYIDETL LKVASRFAGK IDPDNPDTIK 310 320 330 340 350 360 IHRQMVSKEL GIDIKAVEEE LDRAAKVFQI LDHTKTIMLM LADGLVPSNS GEGYLGRLVI 370 380 390 400 410 420 RRALKVLRLL KSDVRLYELV KEQIDFWKED FPQVLKNKDY ILDAVELEQQ RFEKILEKVP 430 440 450 460 470 480 SIASTLARKS EITTEDLIQV YDSNGIPPDL LEEELKKKSV KFELPRNFYA LVAKRHQTST 490 500 510 520 530 540 IKSAYDKVKL PKDMLEYITA LQPTEKLYYK DQYMRSFEGK VLGVYKNYLI LDKTTFYPEG 550 560 570 580 590 600 GGQLGDTGLI IDEKSSKRYE VIDTQKVNDV IVHILKEEPS TIKVGDNVRG EINWERRYRL 610 620 630 640 650 660 MRHHTVTHVI LAAAKKVLGE HVWQAGAEKT PEKGRLDITH HKTLTEEEVK LIENYANSVI 670 680 690 700 710 720 SDRRQVKPLE MNRMEAEMKY GVSIYEGGVP NSATIRLLEI KDWDIESCGG THVSNTSEIG 730 740 750 760 770 780 AVKIINVERI QDGVIRLEYV AGPALVDYIR ETQAKIVEAS KIIGTSPDQL TSRLRRILNE 790 800 810 820 830 840 IEEKNNLIIQ YRRIIETELL NNLKPYEINS NKIYIIEGLG DEEENKEILR KLTSTDNTIA 850 860 870 880 890 900 ISISDNRLQI ATSKNMRVDK IVEELLKGGG KGGGKGTFAN VILNSKKSKE EIIEIVRKSL

References

[1]	"Biogeography of the Sulfolobus islandicus pan-genome." Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J. Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610(2009) [PubMed: 19435847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Y.G.57.14 / Yellowstone #1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001403 Genomic DNA. Translation: ACP46137.1.
RefSeq	YP_002838059.1. NC_012622.1.
3D structure databases
ProteinModelPortal	C3N7E3.
ModBase	Search...
Protein-protein interaction databases
STRING	C3N7E3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7807284.
GenomeReviews	Gene locus YG5714_1881 in contig CP001403_GR.
KEGG	siy:YG5714_1881.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GESKTDQ.
ProtClustDB	PRK13902.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_SULIY

Accession

Primary (citable) accession number: C3N7E3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program