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C3N6N2 (ENDA_SULIA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease
Gene names
Name:endA
Ordered Locus Names:M1627_1782
OrganismSulfolobus islandicus (strain M.16.27) [Complete proteome] [HAMAP]
Taxonomic identifier427318 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length182 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01833

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. HAMAP-Rule MF_01833

Subunit structure

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.

Sequence similarities

Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionlyase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 182182tRNA-splicing endonuclease HAMAP-Rule MF_01833
PRO_1000216079

Sites

Active site1191 By similarity
Active site1271 By similarity
Active site1581 By similarity

Sequences

Sequence LengthMass (Da)Tools
C3N6N2 [UniParc].

Last modified June 16, 2009. Version 1.
Checksum: A155C4FA4C666054

FASTA18220,798
        10         20         30         40         50         60 
MVKALLVGSK VLIPNVDESR YIYSNGFYGK AIGISKPKGP KDIIRPLELS LIESVYLAKK 

        70         80         90        100        110        120 
GLIKVIDKNG EVLEYEKLYE YSSKIINKFD IMYRVYEDLR EKGFIVRSGV KYGADFAVYT 

       130        140        150        160        170        180 
LGPGLEHAPY VVIAVDIDEE ITPHELLSFG RVSHSTRKRL VLALVDRKSE SVRYIMFKWV 


KM 

« Hide

References

[1]"Biogeography of the Sulfolobus islandicus pan-genome."
Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J.
Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: M.16.27.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001401 Genomic DNA. Translation: ACP55657.1.
RefSeqYP_002843702.1. NC_012632.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING427318.M1627_1782.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACP55657; ACP55657; M1627_1782.
GeneID7812446.
KEGGsim:M1627_1782.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1676.
HOGENOMHOG000107823.
KOK01170.
OMAALKFGSH.

Enzyme and pathway databases

BioCycSISL427318:GH3D-1848-MONOMER.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
HAMAPMF_01833. EndA_short.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFPIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsTIGR00324. endA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameENDA_SULIA
AccessionPrimary (citable) accession number: C3N6N2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: June 11, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families