C3MZR1 (DNLI_SULIA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	M1627_2023

Organism

Sulfolobus islandicus (strain M.16.27) [Complete proteome] [HAMAP]

Taxonomic identifier

427318 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus islandicus

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	DNA ligase HAMAP-Rule MF_00407		PRO_1000205955
Sites
	Active site	260	1	N6-AMP-lysine intermediate By similarity
	Binding site	258	1	ATP By similarity
	Binding site	265	1	ATP By similarity
	Binding site	280	1	ATP By similarity
	Binding site	310	1	ATP By similarity
	Binding site	350	1	ATP By similarity
	Binding site	427	1	ATP By similarity
	Binding site	433	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	C3MZR1 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 0D257FC878FA12FC	FASTA	601	67,716
	10 20 30 40 50 60 MEFKVIAEYF DKLEKISSRL QLTALLADLL SKSDKAIIDK VVYIIQGKLW PDFLGYPELG 70 80 90 100 110 120 IGEKFLIKAI SIATNTDENS VENLYKSIGD LGEVARRLKS KQQSTGILGF LGTSSKESLK 130 140 150 160 170 180 VDEVYSTLSK VALTTGEGSR DLKIRLLAGL LKKADPLEAK FLVRFVEGRL RVGIGDATVL 190 200 210 220 230 240 DAMAIAFGGG QSASEIVERA YNLRADLGNI AKIIVEKGIE ALKTLKPEVG IPIRPMLAER 250 260 270 280 290 300 LSNPEEILKK VGGSALVDYK YDGERAQIHK KDDKIFIFSR RLENITSQYP DVVEYISKYT 310 320 330 340 350 360 EGKEFIIEGE IVAVDPESGE MRSFQELMHR KRKSDIYEAI KEYPVNVFLF DLMYYEDVDY 370 380 390 400 410 420 TTKPLEVRRK LLESIVKPND YVKIAHHIQV NNVEDLKSFF YRAISEGGEG VMVKAIGKDA 430 440 450 460 470 480 IYQAGARGWL WIKLKRDYQS EMADTVDLVV VGGFYGKGKR GGKISSLLMA AYNPKTDTFE 490 500 510 520 530 540 SVCKVASGFS DEQLDELQKK LMEIKRDIKH PRVNSKMEPD IWVEPVYVAE IIGAEITISP 550 560 570 580 590 600 LHTCCQDVVE KDAGLSIRFP RFIRWRDDKS PEDATTTDEI LEMYNKQPKK KIESPPIDES V

References

[1]	"Biogeography of the Sulfolobus islandicus pan-genome." Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J. Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: M.16.27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001401 Genomic DNA. Translation: ACP55893.1.
RefSeq	YP_002843938.1. NC_012632.1.
3D structure databases
ProteinModelPortal	C3MZR1.
ModBase	Search...
Protein-protein interaction databases
STRING	427318.M1627_2023.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACP55893; ACP55893; M1627_2023.
GeneID	7812129.
KEGG	sim:M1627_2023.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1793.
HOGENOM	HOG000036008.
KO	K10747.
OMA	AQVACLY.
ProtClustDB	PRK01109.
Enzyme and pathway databases
BioCyc	SISL427318:GH3D-2104-MONOMER.
Family and domain databases
Gene3D	1.10.3260.10. 1 hit. 2.40.50.140. 1 hit.
HAMAP	MF_00407. DNA_ligase.
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_SULIA

Accession

Primary (citable) accession number: C3MZR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program