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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41NADUniRule annotation1
Binding sitei68NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei132NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi162Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi177IronUniRule annotation1
Binding sitei227NAD; via amide nitrogenUniRule annotation1
Binding sitei237GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi60 – 61NADUniRule annotation2
Nucleotide bindingi160 – 162NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:M1425_1670
OrganismiSulfolobus islandicus (strain M.14.25 / Kamchatka #1)
Taxonomic identifieri427317 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001350 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002050081 – 267Thiamine thiazole synthaseAdd BLAST267

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Structurei

3D structure databases

SMRiC3MWW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG094A0142.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

C3MWW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVKIKQVEE VKISRYIIKE TMEDWYQFVE SDVVIVGAGP SGLSAAYYLA
60 70 80 90 100
KAGLKTLVFE RRLSFGGGIG GGAMLFHKLI IEKPADEILR EVNVRLKEVE
110 120 130 140 150
EGVYVVDSAE FMAKLATAAI DAGAKIIHGV TVDDVIFREN PLRVAGVAVE
160 170 180 190 200
WTATQMASLH VDPIFISAKA VVDATGHDAE VISVAARKIP ELGIVIPGEK
210 220 230 240 250
SAYSERAEEL TVINTGKVAE GLYAAGMAVT EVKGLPRMGP IFGAMVLSGK
260
AVAEEITKDL LKSEIRT
Length:267
Mass (Da):28,702
Last modified:June 16, 2009 - v1
Checksum:i4EB28B391187655A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001400 Genomic DNA. Translation: ACP38419.1.
RefSeqiWP_012711650.1. NC_012588.1.

Genome annotation databases

EnsemblBacteriaiACP38419; ACP38419; M1425_1670.
GeneIDi7794128.
KEGGisia:M1425_1670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001400 Genomic DNA. Translation: ACP38419.1.
RefSeqiWP_012711650.1. NC_012588.1.

3D structure databases

SMRiC3MWW9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP38419; ACP38419; M1425_1670.
GeneIDi7794128.
KEGGisia:M1425_1670.

Phylogenomic databases

HOGENOMiHOG000106048.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG094A0142.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_SULIM
AccessioniPrimary (citable) accession number: C3MWW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: February 15, 2017
This is version 49 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.