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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei600ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:M1425_1418
OrganismiSulfolobus islandicus (strain M.14.25 / Kamchatka #1)
Taxonomic identifieri427317 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001350 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002162591 – 1049Isoleucine--tRNA ligaseAdd BLAST1049

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC3MVH6
SMRiC3MVH6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 59"HIGH" regionAdd BLAST12
Motifi597 – 601"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

C3MVH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPLTGNYDP KKIEDEIISF WEENKIYNKL RDIVSKRREK FLFIDGPPYP
60 70 80 90 100
SSPTPHIGTI WNKVIKDCIL RYERLLGKKV HDQPGYDTHG LPIEVATERL
110 120 130 140 150
LGILNKQEII DKIGVENFIN KCKEFALSNA TKMTQNFKDV GVFMDWERPY
160 170 180 190 200
YTLDPSYISS SWSVIKKAYE KGMLDKGTAV LHWCPRCETT LSDYEVSEYR
210 220 230 240 250
DLEDPSIYVK FKIKGEENRY LLIWTTTPWT IPSNVFVMVN KDYDYADVEV
260 270 280 290 300
NGEVLVLAKD RIEAVMKEAS ITNYKVLRTY KGSELIGVKY KHPLRDFVSA
310 320 330 340 350
QTKLDDFHQV VDAGNVVTLT DGTGLVHAAT GHGEEDFLIG QKYGFPVVMF
360 370 380 390 400
VNDRGEFTEE GGKYKGLKVR DASKVIINDL KSKNALFFEG KIVHRYPVCW
410 420 430 440 450
RCKTPLVLRA IDQWFIRVTK IKDEMLKEIE NVNWIPDWGK SRISNMVKEL
460 470 480 490 500
RDWVISRQRF WGTPLPIWIC EKCNNVIVVG SREDLESIAI DSVPNDLHRP
510 520 530 540 550
WIDNVRVKCN KCGGVAKRIA DVADVWFDSG VAFFASLGKD WQEKWKELGP
560 570 580 590 600
VDLVLEGHDQ LRGWFFSLLR SGLILLDKAP YVSVLVHGFM LDEQGREMHK
610 620 630 640 650
SLGNYVEPSV VIQRYGRDIL RLWLLRNTTW EDARFSWRAL ELTKRDLQII
660 670 680 690 700
WNTYVFASMY MNLDNFEPVK YTLDDVIKYA KIEDLWILSR FNSMLKKVNE
710 720 730 740 750
SMKNYKVHEM ANYLINFLVE DVSRFYIRLI RKRAWIEANT QDKIAMYYIL
760 770 780 790 800
YFILKQWIIL ASTIIPFISE KIYKSFVVNP KESVSMESSI NYDERFIDNE
810 820 830 840 850
LERAFEVARE INEASLNARA KAGIKLRWPL AKVYIFVEDE DTLAKVNRIK
860 870 880 890 900
DVLLSLLNAK DIEISKIEGF KSFSKYKVEP NRSIIGKEYK SMSPKILDYI
910 920 930 940 950
RNNSDIIAID ILNKKQHVAR IDNVDVILNT SHVIISEETI EGYVSSKFAQ
960 970 980 990 1000
GIVVISKEIS ESEEEEGLVR DIIRRIQFMR KQLKLNVVDY IEISIKAPEE
1010 1020 1030 1040
RVKTIQKWEE FIKSETRGNK VILGDPKGDI VMDWDIEGES YIIGIKKST
Length:1,049
Mass (Da):121,984
Last modified:June 16, 2009 - v1
Checksum:i6E9C7F840E1C9E40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001400 Genomic DNA Translation: ACP38171.1

Genome annotation databases

EnsemblBacteriaiACP38171; ACP38171; M1425_1418
KEGGisia:M1425_1418

Similar proteinsi

Entry informationi

Entry nameiSYI_SULIM
AccessioniPrimary (citable) accession number: C3MVH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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