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C3MVH6

- SYI_SULIM

UniProt

C3MVH6 - SYI_SULIM

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Protein
Isoleucine--tRNA ligase
Gene
ileS, M1425_1418
Organism
Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei600 – 6001ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSISL427317:GI7C-1463-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:M1425_1418
OrganismiSulfolobus islandicus (strain M.14.25 / Kamchatka #1)
Taxonomic identifieri427317 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001350: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10491049Isoleucine--tRNA ligaseUniRule annotation
PRO_1000216259Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi427317.M1425_1418.

Structurei

3D structure databases

ProteinModelPortaliC3MVH6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5912"HIGH" regionUniRule annotation
Add
BLAST
Motifi597 – 6015"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKKRIELM.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3MVH6-1 [UniParc]FASTAAdd to Basket

« Hide

MKPLTGNYDP KKIEDEIISF WEENKIYNKL RDIVSKRREK FLFIDGPPYP     50
SSPTPHIGTI WNKVIKDCIL RYERLLGKKV HDQPGYDTHG LPIEVATERL 100
LGILNKQEII DKIGVENFIN KCKEFALSNA TKMTQNFKDV GVFMDWERPY 150
YTLDPSYISS SWSVIKKAYE KGMLDKGTAV LHWCPRCETT LSDYEVSEYR 200
DLEDPSIYVK FKIKGEENRY LLIWTTTPWT IPSNVFVMVN KDYDYADVEV 250
NGEVLVLAKD RIEAVMKEAS ITNYKVLRTY KGSELIGVKY KHPLRDFVSA 300
QTKLDDFHQV VDAGNVVTLT DGTGLVHAAT GHGEEDFLIG QKYGFPVVMF 350
VNDRGEFTEE GGKYKGLKVR DASKVIINDL KSKNALFFEG KIVHRYPVCW 400
RCKTPLVLRA IDQWFIRVTK IKDEMLKEIE NVNWIPDWGK SRISNMVKEL 450
RDWVISRQRF WGTPLPIWIC EKCNNVIVVG SREDLESIAI DSVPNDLHRP 500
WIDNVRVKCN KCGGVAKRIA DVADVWFDSG VAFFASLGKD WQEKWKELGP 550
VDLVLEGHDQ LRGWFFSLLR SGLILLDKAP YVSVLVHGFM LDEQGREMHK 600
SLGNYVEPSV VIQRYGRDIL RLWLLRNTTW EDARFSWRAL ELTKRDLQII 650
WNTYVFASMY MNLDNFEPVK YTLDDVIKYA KIEDLWILSR FNSMLKKVNE 700
SMKNYKVHEM ANYLINFLVE DVSRFYIRLI RKRAWIEANT QDKIAMYYIL 750
YFILKQWIIL ASTIIPFISE KIYKSFVVNP KESVSMESSI NYDERFIDNE 800
LERAFEVARE INEASLNARA KAGIKLRWPL AKVYIFVEDE DTLAKVNRIK 850
DVLLSLLNAK DIEISKIEGF KSFSKYKVEP NRSIIGKEYK SMSPKILDYI 900
RNNSDIIAID ILNKKQHVAR IDNVDVILNT SHVIISEETI EGYVSSKFAQ 950
GIVVISKEIS ESEEEEGLVR DIIRRIQFMR KQLKLNVVDY IEISIKAPEE 1000
RVKTIQKWEE FIKSETRGNK VILGDPKGDI VMDWDIEGES YIIGIKKST 1049
Length:1,049
Mass (Da):121,984
Last modified:June 16, 2009 - v1
Checksum:i6E9C7F840E1C9E40
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001400 Genomic DNA. Translation: ACP38171.1.
RefSeqiYP_002829469.1. NC_012588.1.

Genome annotation databases

EnsemblBacteriaiACP38171; ACP38171; M1425_1418.
GeneIDi7793866.
KEGGisia:M1425_1418.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001400 Genomic DNA. Translation: ACP38171.1 .
RefSeqi YP_002829469.1. NC_012588.1.

3D structure databases

ProteinModelPortali C3MVH6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 427317.M1425_1418.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACP38171 ; ACP38171 ; M1425_1418 .
GeneIDi 7793866.
KEGGi sia:M1425_1418.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KKRIELM.

Enzyme and pathway databases

BioCyci SISL427317:GI7C-1463-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: M.14.25 / Kamchatka #1.

Entry informationi

Entry nameiSYI_SULIM
AccessioniPrimary (citable) accession number: C3MVH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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