C3MUW9 (C3MUW9_SULIM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

M1425_0477

Organism

Sulfolobus islandicus (strain M.14.25 / Kamchatka #1) [Complete proteome] [HAMAP]

Taxonomic identifier

427317 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	255	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	286	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	380	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	C3MUW9 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: C13FA0B77E52F85A	FASTA	561	64,358
	10 20 30 40 50 60 MEFGYRLDRN GVTFNLWAPY QRNVKLKILN KGIYEMERDE KGYFTITLNN VKVGDRYKYI 70 80 90 100 110 120 LDDGSEVPDP ASRYQPEGVH GHSEIISLDF KGDDENGVKV KREDLIIYEL HTSTFTSEGT 130 140 150 160 170 180 FEGVIKKLDY LKELGVTAIE IMPIAQFPGK KDWGYDGVYL YAVQNSYGGP IGFRNLVNEA 190 200 210 220 230 240 HKLGLAIILD VVYNHVGPEG NYMAKLGPYF SEKYKTPWGL AFNFDDAGSD EVRKFILENV 250 260 270 280 290 300 EYWINEFHVD GFRLDAVHAI IDNSPKHILE DIADVVHKYG KIVITESDLN DPRVVNPKEK 310 320 330 340 350 360 CGYNTDAQWV DDFHHAIHAF LTGERQGYYS DFGSLDDIVK SYKEVFVYDG KYSNFRRKTH 370 380 390 400 410 420 GKPVGDLDGC KFVVYIQNHD QVGNRGGGER LIKLVDKESY KIAAALYMLS PYIPMIFMGE 430 440 450 460 470 480 EYGEENPFYY FSDFSDPKLI QGVKEGRKRD NGQETDPQSD STFNASKLSW KINDDIFSFY 490 500 510 520 530 540 KTLIKIRKEY GLACNRKLTV ENGNHWLTVK GNGYLVVYVF SQSIIEMKYR GTLVLSSNNS 550 560 FPSQIVENKY KLEKGFALYK L

References

[1]	"Biogeography of the Sulfolobus islandicus pan-genome." Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J. Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610(2009) [PubMed: 19435847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001400 Genomic DNA. Translation: ACP37332.1.
RefSeq	YP_002828630.1. NC_012588.1.
3D structure databases
ProteinModelPortal	C3MUW9.
ModBase	Search...
Protein-protein interaction databases
STRING	C3MUW9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7796506.
GenomeReviews	Gene locus M1425_0477 in contig CP001400_GR.
KEGG	sia:M1425_0477.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EGFVYQG.
ProtClustDB	CLSK285578.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR015156. Maltooligo_trehalose_arc_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF09071. Alpha-amyl_C. 1 hit. PF00128. Alpha-amylase. 2 hits. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C3MUW9_SULIM

Accession

Primary (citable) accession number: C3MUW9

Entry history

Integrated into UniProtKB/TrEMBL:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	November 16, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)