C3MQL0 (SYP_SULIL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	LS215_1669

Organism

Sulfolobus islandicus (strain L.S.2.15 / Lassen #1) [Complete proteome] [HAMAP]

Taxonomic identifier

429572 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Proline--tRNA ligase HAMAP MF_01571		PRO_1000215577

Sequences

	Sequence		Length	Mass (Da)
	C3MQL0 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: CD9522617108D6A4	FASTA	481	55,678
	10 20 30 40 50 60 MQITRDKWSK NFSEWFDWVL REGEFYDYGR YPVKGMGVWM PYGFKLRQNI ISIIRNLLDS 70 80 90 100 110 120 TGHEEVLFPL LIPEDLLRRE STHIKGFEEE VFWVTKGGSE DLDVKLALRP TSEVAITTME 130 140 150 160 170 180 NLWLKSYKQL PKKYYQIVSV FRYETKATRP MIRLREITTF KEAHTVHETY DDAQRQVEEA 190 200 210 220 230 240 IEIYKKIFNN LAIPYVLSER PEWDRFAGAL HTYAFDTIMP DGKVMQIGTV HHLGQNFSRA 250 260 270 280 290 300 LDFKIQKKDG SLDYPHQTSY GISDRAIASV IAIHGDDHGP ILPPSVAPIK VVVVPIPAKN 310 320 330 340 350 360 EEGTQQVMKY SIEICEMLNK NNITCVTDQD TEKTPGEKFY IWEIKGVPIR LEIGPRELAS 370 380 390 400 410 420 STVFIKRRDN LKSYTVKKEE VVNKVKEVLN EIQEDLRKRA WESLKSRIEY ANDIEKAKNI 430 440 450 460 470 480 LENNSGIVDV PWCGSKECGL KIEELTNARV LGYPIEDRKV NDKCVICKMN AKTVLRVAKT Y

References

[1]	"Biogeography of the Sulfolobus islandicus pan-genome." Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J. Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610(2009) [PubMed: 19435847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L.S.2.15 / Lassen #1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001399 Genomic DNA. Translation: ACP35673.1.
RefSeq	YP_002832318.1. NC_012589.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C3MQL0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7799303.
GenomeReviews	Gene locus LS215_1669 in contig CP001399_GR.
KEGG	sis:LS215_1669.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KFAEYEL.
ProtClustDB	PRK08661.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_SULIL

Accession

Primary (citable) accession number: C3MQL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program