C3MPN7 (TOP6B_SULIL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type 2 DNA topoisomerase 6 subunit B
EC=5.99.1.3

Alternative name(s):
Type II DNA topoisomerase VI subunit B
Short name=TopoVI-B

Gene names

Name:	top6B
Ordered Locus Names:	LS215_1342

Organism

Sulfolobus islandicus (strain L.S.2.15 / Lassen #1) [Complete proteome] [HAMAP]

Taxonomic identifier

429572 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus islandicus

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Relaxes both positive and negative superturns and exhibits a strong decatenase activity By similarity. HAMAP-Rule MF_00322
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_00322
Subunit structure	Homodimer. Heterotetramer of two Top6A and two Top6B chains By similarity.
Sequence similarities	Belongs to the TOP6B family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Type 2 DNA topoisomerase 6 subunit B HAMAP-Rule MF_00322		PRO_1000205144
Regions
	Nucleotide binding	97 – 98	2	ATP By similarity
	Nucleotide binding	106 – 113	8	ATP By similarity
Sites
	Binding site	42	1	ATP By similarity
	Binding site	76	1	ATP By similarity
	Binding site	427	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	C3MPN7 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: A89C3767142BF421	FASTA	530	60,563
	10 20 30 40 50 60 MSAKEKFTSL SPAEFFKRNP ELAGFPNPAR ALYQTVRELI ENSLDATDVH GILPNIKITI 70 80 90 100 110 120 DLIDEARQIY KVNVVDNGIG IPPQEVPNAF GRVLYSSKYV NRQTRGMYGL GVKAAVLYSQ 130 140 150 160 170 180 MHQDKPIEIE TSPANSKRIY TFKLKIDINK NEPIIVERGS VENTRGFHGT SVAISIPGDW 190 200 210 220 230 240 PKAKSRIYEY IKRTYIITPY AEFIFKDPEG NVTYYPRLTN KIPKPPQEVK PHPYGVDREE 250 260 270 280 290 300 IKILINNLKR DYTIKEFLVN EFQSIGDTTA DKILELAGLK PNKKVKNLTE EEITRLVETF 310 320 330 340 350 360 KKYEDFRSPS ADSLSVIGED LIELGLKKIF NPDFAASITR KPKAYQGHPF IVEAGVAFGG 370 380 390 400 410 420 SIPVGEEPIV LRYANKIPLI YDEKSDVIWK VVEELDWKRY GIESDQYQMV VMVHLCSTKI 430 440 450 460 470 480 PYKSAGKESI AEVEDIEKEI KNALMEVARK LKQYLSEKRK EQEAKKKLLA YLKYIPEVSR 490 500 510 520 530 SLATFLASGN KELVSKYQNE ISEGLFKLIS KKLDLINIEE YRKVYRVDSE

References

[1]	"Biogeography of the Sulfolobus islandicus pan-genome." Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J. Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L.S.2.15 / Lassen #1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001399 Genomic DNA. Translation: ACP35350.1.
RefSeq	YP_002831995.1. NC_012589.1.
3D structure databases
ProteinModelPortal	C3MPN7.
ModBase	Search...
Protein-protein interaction databases
STRING	429572.LS215_1342.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACP35350; ACP35350; LS215_1342.
GeneID	7797858.
KEGG	sis:LS215_1342.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1389.
HOGENOM	HOG000225919.
KO	K03167.
OMA	IQSRGQQ.
ProtClustDB	PRK04184.
Enzyme and pathway databases
BioCyc	SISL429572:GHOW-1384-MONOMER.
Family and domain databases
Gene3D	3.30.230.10. 1 hit. 3.30.565.10. 1 hit.
HAMAP	MF_00322. Top6B.
InterPro	IPR008616. Fibro-bd_N. IPR003594. HATPase_ATP-bd. IPR010979. Ribosomal_S13-like_H2TH. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005734. TopoVI_B. IPR015320. TopoVI_B_transducer. [Graphical view]
Pfam	PF05833. FbpA. 1 hit. PF09239. Topo-VIb_trans. 1 hit. [Graphical view]
PIRSF	PIRSF006553. TopoVI_B. 1 hit.
ProDom	PD013790. TopoVI_B_transducer. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF46946. Ribosomal_H2TH. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01052. top6b. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TOP6B_SULIL

Accession

Primary (citable) accession number: C3MPN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program