C3MHD5 (SYGB_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase beta subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS

Gene names

Name:	glyS
Ordered Locus Names:	NGR_c04670

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium fredii group › Rhizobium fredii

Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00255
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 721	721	Glycine--tRNA ligase beta subunit HAMAP-Rule MF_00255		PRO_1000197212

Sequences

	Sequence		Length	Mass (Da)
	C3MHD5 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: A18A6D2A5091231B	FASTA	721	79,281
	10 20 30 40 50 60 MPDLLIELRS EEIPARMQRK AAGDLKKLVT DALVEAGLTY EGAREYWTPR RLTLDIRGLN 70 80 90 100 110 120 ARSADVREER KGPRTDANEK AIEGFLRAAG LSSIGDAHVH SDPKKGDFYV AHIVKPGRDA 130 140 150 160 170 180 EDIVAEVMPA IIRDFPWPKS MRSGAASAKP GSLRWVRPLQ SIVCTFGAET EETRVIPFAV 190 200 210 220 230 240 DGITASNITY GHRFHAPEAI MVRRFADYAD KLEKAKVVLD AERRKEIIGA DARNIAFANG 250 260 270 280 290 300 LELVEDEGLL EEVTGLVEWP RVLMGSFEES YLEIPSEIIR LTIKTNQKCF VTRQPGAETL 310 320 330 340 350 360 SNRFILVSNI EAKDGGKEIV HGNGKVVRAR LSDAAHFWKR DQGDLPDLET LKPSAEKFGL 370 380 390 400 410 420 DLGKPLDQRM AKLDALNVTF HAKLGTQGER VARIRSIALA LAEVTGADQA LVERSVVLAK 430 440 450 460 470 480 ADLRTEAVGE FPELQGIMGH KYAVLQGEDA AVANAIEDHY KPQGPSDRVP TDAVSAAVAL 490 500 510 520 530 540 ADKLDTLAGF WAIDEKPTGS KDPYALRRAA LGVIRLILEG RTRLPLLPFF DVALKALRTQ 550 560 570 580 590 600 RPDLTGDIAA DLLAFFHDRL KVYLRDLGAR YDLIDAVLTS EADDLLMIAR RVEALTAFIT 610 620 630 640 650 660 AEEGKNLLAG TKRATQILAA EEKKGTEVAD QVDERLFRLD AERTLHASIK LASDDAREAI 670 680 690 700 710 720 GKEDFRSAMA ALSKLREPVD VFFNDVLVND EDRAIRANRL ALLGLIRSAT GEVADFSKIS G

References

[1]

"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001389 Genomic DNA. Translation: ACP24263.1.
RefSeq	YP_002825016.1. NC_012587.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	394.NGR_c04670.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACP24263; ACP24263; NGR_c04670.
GeneID	7791693.
KEGG	rhi:NGR_c04670.
PATRIC	32307299. VBIRhiSp122450_3275.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0751.
HOGENOM	HOG000264304.
KO	K01879.
OMA	GMHYARL.
ProtClustDB	PRK01233.
Family and domain databases
HAMAP	MF_00255. Gly_tRNA_synth_beta.
InterPro	IPR008909. DALR_anticod-bd. IPR015944. Gly-tRNA-synth_bsu. IPR006194. Gly-tRNA-synth_heterodimer. [Graphical view]
Pfam	PF05746. DALR_1. 1 hit. PF02092. tRNA_synt_2f. 1 hit. [Graphical view]
PRINTS	PR01045. TRNASYNTHGB.
TIGRFAMs	TIGR00211. glyS. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGB_RHISN

Accession

Primary (citable) accession number: C3MHD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program