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C3MCY7 (FPG_RHISN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Formamidopyrimidine-DNA glycosylase

Short name=Fapy-DNA glycosylase
EC=3.2.2.23
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutM
Short name=AP lyase MutM
EC=4.2.99.18
Gene names
Name:mutM
Synonyms:fpg
Ordered Locus Names:NGR_c37140
OrganismRhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]
Taxonomic identifier394 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. HAMAP-Rule MF_00103

Catalytic activity

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine. HAMAP-Rule MF_00103

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00103

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00103

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00103

Sequence similarities

Belongs to the FPG family.

Contains 1 FPG-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 296295Formamidopyrimidine-DNA glycosylase HAMAP-Rule MF_00103
PRO_1000118898

Regions

Zinc finger260 – 29637FPG-type HAMAP-Rule MF_00103

Sites

Active site21Schiff-base intermediate with DNA By similarity
Active site31Proton donor By similarity
Active site581Proton donor; for beta-elimination activity By similarity
Active site2861Proton donor; for delta-elimination activity By similarity
Binding site1041DNA By similarity
Binding site1261DNA By similarity
Binding site1691DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
C3MCY7 [UniParc].

Last modified June 16, 2009. Version 1.
Checksum: 7DCB947600FEBC2D

FASTA29632,144
        10         20         30         40         50         60 
MPELPEVETV KRGLAPAIEG ALLVRAELRR PDLRFPFPEN FAAAVSGRRI LALSRRAKYL 

        70         80         90        100        110        120 
MIDLEGGDVI VAHLGMSGSF RIEAGAQPAA PGEFHHPRGK DEKHDHVIFH LDGGSGQMRV 

       130        140        150        160        170        180 
IYNDPRRFGF MDLARRDTIA EHAYFRDLGE EPTGNALDAA YLAARLAGKS QPLKTALLDQ 

       190        200        210        220        230        240 
RTIAGLGNIY VCEALWRSGL SPNRAAGTLV DKRGRPKQAL LALVEAIRAV IADAIAAGGS 

       250        260        270        280        290 
SLKDHIQADG SLGYFQHAFS VYDRAGEACR KPGCDGTVTR IVQAGRSTFH CPRCQK 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001389 Genomic DNA. Translation: ACP27435.1.
RefSeqYP_002828188.1. NC_012587.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394.NGR_c37140.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACP27435; ACP27435; NGR_c37140.
GeneID7793629.
KEGGrhi:NGR_c37140.
PATRIC32313998. VBIRhiSp122450_6569.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0266.
HOGENOMHOG000020881.
KOK10563.
OMAQNTLGMN.
OrthoDBEOG6QP131.

Enzyme and pathway databases

BioCycSFRE394:GBYN-3698-MONOMER.

Family and domain databases

HAMAPMF_00103. Fapy_DNA_glycosyl.
InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR000191. DNA_glycosylase/AP_lyase.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
TIGRFAMsTIGR00577. fpg. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFPG_RHISN
AccessionPrimary (citable) accession number: C3MCY7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 16, 2009
Last modified: May 14, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families