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Protein

Malate synthase G

Gene

glcB

Organism
Sinorhizobium fredii (strain NBRC 101917 / NGR234)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei274Acetyl-CoAUniRule annotation1
Binding sitei311Acetyl-CoAUniRule annotation1
Active sitei338Proton acceptorUniRule annotation1
Binding sitei338GlyoxylateUniRule annotation1
Metal bindingi430MagnesiumUniRule annotation1
Binding sitei430GlyoxylateUniRule annotation1
Metal bindingi458MagnesiumUniRule annotation1
Binding sitei539Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei629Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:NGR_c34110
OrganismiSinorhizobium fredii (strain NBRC 101917 / NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001054 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001474271 – 724Malate synthase GAdd BLAST724

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei615Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi394.NGR_c34110

Structurei

3D structure databases

SMRiC3MBD2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 124Acetyl-CoA bindingUniRule annotation2
Regioni455 – 458Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3 Bacteria
COG2225 LUCA
HOGENOMiHOG000220740
KOiK01638
OMAiTEPVLHA
OrthoDBiPOG091H05P0

Family and domain databases

CDDicd00728 malate_synt_G, 1 hit
Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

C3MBD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRVEKHDLK IDAGLHRFLV DEAMPGTGID PEHFFAALSA LVHDLAPKNR
60 70 80 90 100
ALLARRDELQ AKLDAWYREH GAPVDLDAYQ AFLKEIGYLL PEGPDFSVST
110 120 130 140 150
ANVDAEIATI AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAIPDAD
160 170 180 190 200
GGTRGRSYNP ARGAKVIAWA RDFLDASVPL AAGRWSDVGG LAIDGTALSL
210 220 230 240 250
TLTNGTKTTL RNPAQFAGYS GSAEAPSQIV LRQNNLHVVI VLDATTPIGQ
260 270 280 290 300
ADPAGISDII LESAITTIMD CEDSVAAVDA EDKVVVYRNW LGLMKGDLAE
310 320 330 340 350
EVAKGGRTFT RKLNADRVFT RPNGETLTLP GRALMLVRNV GHLMTNPAIL
360 370 380 390 400
DRDGREVPEG LMDAMVTALI ALHDIGANGR RANSRAGSMY VVKPKMHGPE
410 420 430 440 450
EVAFACEIFS HVEAALGLPE NTIKMGIMDE ERRTTVNLKE CIRAARDRVV
460 470 480 490 500
FINTGFLDRT GDEIHTSMEA GPMIRKGDMK QAAWIGAYEN WNVDIGLECG
510 520 530 540 550
LSGRAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS PTAATLHATH
560 570 580 590 600
YHRVDVAEVQ AGLGSRPRAR LADILSVPVA ARPNWTEEEI QRELDNNAQG
610 620 630 640 650
ILGYVVRWVD EGVGCSKVPD INNVGLMEDR ATLRISAQHM ANWLHHGIVS
660 670 680 690 700
KVQIVETMKR MAEIVDRQNA GDPSYVPMAG RLEQSIAFQA ALDLVLKGRE
710 720
QPNGYTEPVL HRRRLELKAK QRAS
Length:724
Mass (Da):78,795
Last modified:June 16, 2009 - v1
Checksum:iD90D8C146A321A1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001389 Genomic DNA Translation: ACP27141.1
RefSeqiWP_012709888.1, NC_012587.1
YP_002827894.1, NC_012587.1

Genome annotation databases

EnsemblBacteriaiACP27141; ACP27141; NGR_c34110
GeneIDi7792678
KEGGirhi:NGR_c34110
PATRICifig|394.7.peg.6262

Similar proteinsi

Entry informationi

Entry nameiMASZ_SINFN
AccessioniPrimary (citable) accession number: C3MBD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 16, 2009
Last modified: April 25, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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