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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSFRE394:GBYN-3323-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:NGR_c33390
OrganismiRhizobium sp. (strain NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001054 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Leucine--tRNA ligasePRO_1000199220Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi394.NGR_c33390.

Structurei

3D structure databases

ProteinModelPortaliC3MB60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi632 – 6365"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3MB60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATERYNPRD TEPRWQQEWD ARKVFETEND DPREKYYVLE MFPYPSGRIH
60 70 80 90 100
MGHVRNYTMG DVVARYKRAR GFNVLHPMGW DAFGMPAENA AMERGVHPAG
110 120 130 140 150
WTYQNIAAMK AQLKVMGLSL DWSREFATCD PAYYQRQQYL FLDFLEKGLV
160 170 180 190 200
YRRQSKVNWD PVDNTVLANE QVIDGRGWRS GALVEQRELT QWFFRITDFS
210 220 230 240 250
QDLLDALDGL EQWPEKVRLM QKNWIGRSEG LALRWALDPA TVPGETKELT
260 270 280 290 300
VYTTRPDTLF GASFLAISAD HPLAREAAAK DAAIDAFCEE CRRAGTSLAA
310 320 330 340 350
LETAEKKGID TGIRARHPFD PEWELPVYVA NFVLMDYGTG AIFGCPSGDQ
360 370 380 390 400
RDLDFARKYG LSVVPVVMPK DADAATFTIE DEAYGGDGVM INSRFLDGLS
410 420 430 440 450
TEEAFEAVAS KLEQETLDGA PRAERKVNFR LRDWGISRQR YWGCPIPVIH
460 470 480 490 500
CEDCGVVPVP KADLPVTLPP DVTFDKPGNP LDRHPTWRHV ACPQCGKDAR
510 520 530 540 550
RETDTMDTFV DSSWYYARFT APWEDKPTDP KAANHWLPVD QYIGGIEHAI
560 570 580 590 600
LHLLYSRFFT RAMKATGHVA MDEPFKGLFT QGMVVHETYS RGEGAQREWV
610 620 630 640 650
TPAEIRIDEI DGRRRAVLIE TGEEVAIGSI EKMSKSKKNV VDPDDIIGSY
660 670 680 690 700
GADTARFFVL SDSPPDRDVI WSEAGVEGAH RFVQRVWRLV GEAAESLRGA
710 720 730 740 750
KASPAKEGEG LAISQAAHRT LRAVEADYDK LAFNKAVARI YELVNVLAAP
760 770 780 790 800
LTQVAAGKAD QAVTAAVKDA TTILIDLIAP MMPHLAEECW REIGGEGLIA
810 820 830 840 850
ERPWPTFDPA LVVENEITLP VQINGKKRAD LTIARDADQS AIESAVLALD
860 870
AVKAALNGAS PRKIIVVPQR IVNVVV
Length:876
Mass (Da):97,961
Last modified:June 16, 2009 - v1
Checksum:i247F386ACAF60FEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001389 Genomic DNA. Translation: ACP27069.1.
RefSeqiWP_012709816.1. NC_012587.1.
YP_002827822.1. NC_012587.1.

Genome annotation databases

EnsemblBacteriaiACP27069; ACP27069; NGR_c33390.
GeneIDi7792606.
KEGGirhi:NGR_c33390.
PATRICi32313209. VBIRhiSp122450_6184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001389 Genomic DNA. Translation: ACP27069.1.
RefSeqiWP_012709816.1. NC_012587.1.
YP_002827822.1. NC_012587.1.

3D structure databases

ProteinModelPortaliC3MB60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394.NGR_c33390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP27069; ACP27069; NGR_c33390.
GeneIDi7792606.
KEGGirhi:NGR_c33390.
PATRICi32313209. VBIRhiSp122450_6184.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciSFRE394:GBYN-3323-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.

Entry informationi

Entry nameiSYL_RHISN
AccessioniPrimary (citable) accession number: C3MB60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 16, 2009
Last modified: April 29, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.