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Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Sinorhizobium fredii (strain NBRC 101917 / NGR234)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei30 – 301S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei55 – 551S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei77 – 771S-adenosyl-L-methionineUniRule annotation
Binding sitei103 – 1031S-adenosyl-L-methionineUniRule annotation
Binding sitei122 – 1221S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSFRE394:GBYN-896-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotation
Ordered Locus Names:NGR_c08980
OrganismiSinorhizobium fredii (strain NBRC 101917 / NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001054 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Ribosomal RNA small subunit methyltransferase APRO_1000194394Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi394.NGR_c08980.

Structurei

3D structure databases

ProteinModelPortaliC3M9C2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227961.
KOiK02528.
OMAiHYPGRLE.
OrthoDBiEOG66F08Z.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3M9C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALDGLPPL RDVIQRHGLD AKKALGQNFL LDLNLTQKIA RTAGPLEDVT
60 70 80 90 100
VIEVGPGPGG LTRAILALGA RKVVAIERDP RCLPALAEIS AHYPGRLDVV
110 120 130 140 150
EGDALKVDFE RLAEGPVRII ANLPYNVGTQ LLVNWLLPER WPPFWQSMTL
160 170 180 190 200
MIQREVGLRI VAGADDDHYG RLGVLCGWRT KARLAFDVPP QAFTPPPKVT
210 220 230 240 250
STVVHLEPID NPIPCAVSAL EKVTQAAFGQ RRKMLRQSLK SIGGEALLGK
260 270 280 290
AGIDPQRRAE TLTVEEFCRL ANCLLGEKVP RSGAAPLRTG FPSEDR
Length:296
Mass (Da):32,210
Last modified:June 16, 2009 - v1
Checksum:i0760DCED2525D17F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001389 Genomic DNA. Translation: ACP24688.1.
RefSeqiWP_012707472.1. NC_012587.1.
YP_002825441.1. NC_012587.1.

Genome annotation databases

EnsemblBacteriaiACP24688; ACP24688; NGR_c08980.
GeneIDi7792123.
KEGGirhi:NGR_c08980.
PATRICi32308181. VBIRhiSp122450_3713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001389 Genomic DNA. Translation: ACP24688.1.
RefSeqiWP_012707472.1. NC_012587.1.
YP_002825441.1. NC_012587.1.

3D structure databases

ProteinModelPortaliC3M9C2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394.NGR_c08980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP24688; ACP24688; NGR_c08980.
GeneIDi7792123.
KEGGirhi:NGR_c08980.
PATRICi32308181. VBIRhiSp122450_3713.

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227961.
KOiK02528.
OMAiHYPGRLE.
OrthoDBiEOG66F08Z.

Enzyme and pathway databases

BioCyciSFRE394:GBYN-896-MONOMER.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 101917 / NGR234.

Entry informationi

Entry nameiRSMA_SINFN
AccessioniPrimary (citable) accession number: C3M9C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 16, 2009
Last modified: July 6, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.