C3M930 (HGD_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	hmgA
Ordered Locus Names:	NGR_c29980

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium fredii group › Rhizobium fredii

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP-Rule MF_00334
Cofactor	Iron By similarity. HAMAP-Rule MF_00334
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP-Rule MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	Homogentisate 1,2-dioxygenase HAMAP-Rule MF_00334		PRO_1000190375
Sites
	Metal binding	347	1	Iron By similarity
	Metal binding	353	1	Iron By similarity
	Metal binding	383	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	C3M930 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 036CCDAB72DCBD48	FASTA	453	50,598
	10 20 30 40 50 60 MLGKAEKRSE ALAAEALAYM PGFGNDFETE SLPGALPQGQ NSPQKCNYGL YAEQLSGSPF 70 80 90 100 110 120 TAPRGTNERS WLYRIRPSVR HTGRFAKIDY PHWKTAPHIA EHALALGQLR WNPLPEPTGE 130 140 150 160 170 180 LNFLQGIRTM TTAGDVLTQV GMAAHAYVFN ADMVDDYFFN ADGELLIVPE MGALQVFTEL 190 200 210 220 230 240 GKMDVAPSEI CLVPRGTMFK VARLGEEKAW RGYICENYGA KFTLPDRGPI GANCLANPRD 250 260 270 280 290 300 FKTPVAAFED KETPCRVQVK WCGSFHTVEI GHSPLDVVAW HGNYAPYKYD LKTFSPVGAI 310 320 330 340 350 360 LFDHPDPSIF TVLTAPSGEE GTANVDFVLF PPRWLVAEHT FRPPWYHRNI MSEFMGLIHG 370 380 390 400 410 420 RYDAKEEGFV PGGMSLHNMM LAHGPDTSGF EKATNGELKP VKLDNTMAFM FETRFPQQLT 430 440 450 RFAAELETLQ DDYIDCWAGL RKRFNGTPEG DWS

References

[1]

"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001389 Genomic DNA. Translation: ACP26741.1.
RefSeq	YP_002827494.1. NC_012587.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	394.NGR_c29980.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACP26741; ACP26741; NGR_c29980.
GeneID	7792270.
KEGG	rhi:NGR_c29980.
PATRIC	32312499. VBIRhiSp122450_5838.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
KO	K00451.
OMA	AVTKWGL.
ProtClustDB	PRK05341.
Enzyme and pathway databases
BioCyc	SFRE394:GBYN-2987-MONOMER.
UniPathway	UPA00139; UER00339.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
HAMAP	MF_00334. Homogentis_dioxygen.
InterPro	IPR005708. Homogentis_dOase. IPR022950. Homogentis_dOase_bac. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_RHISN

Accession

Primary (citable) accession number: C3M930

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 16, 2009
Last modified:	May 29, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program