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Protein

Homogentisate 1,2-dioxygenase

Gene

hmgA

Organism
Sinorhizobium fredii (strain NBRC 101917 / NGR234)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the catabolism of homogentisate (2,5-dihydroxyphenylacetate or 2,5-OH-PhAc), a central intermediate in the degradation of phenylalanine and tyrosine. Catalyzes the oxidative ring cleavage of the aromatic ring of homogentisate to yield maleylacetoacetate.UniRule annotation

Catalytic activityi

Homogentisate + O2 = 4-maleylacetoacetate.UniRule annotation

Cofactori

Fe cationUniRule annotation

Pathwayi: L-phenylalanine degradation

This protein is involved in step 4 of the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Homogentisate 1,2-dioxygenase (hmgA)
  5. no protein annotated in this organism
  6. no protein annotated in this organism
This subpathway is part of the pathway L-phenylalanine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine, the pathway L-phenylalanine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei304Proton acceptorUniRule annotation1
Metal bindingi347IronUniRule annotation1
Metal bindingi353IronUniRule annotation1
Binding sitei362homogentisateUniRule annotation1
Metal bindingi383IronUniRule annotation1
Binding sitei383homogentisateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00139; UER00339.

Names & Taxonomyi

Protein namesi
Recommended name:
Homogentisate 1,2-dioxygenaseUniRule annotation (EC:1.13.11.5UniRule annotation)
Short name:
HGDOUniRule annotation
Alternative name(s):
Homogentisate oxygenaseUniRule annotation
Homogentisic acid oxidaseUniRule annotation
HomogentisicaseUniRule annotation
Gene namesi
Name:hmgAUniRule annotation
Ordered Locus Names:NGR_c29980
OrganismiSinorhizobium fredii (strain NBRC 101917 / NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001054 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001903751 – 453Homogentisate 1,2-dioxygenaseAdd BLAST453

Interactioni

Subunit structurei

Hexamer; dimer of trimers.UniRule annotation

Protein-protein interaction databases

STRINGi394.NGR_c29980.

Structurei

3D structure databases

ProteinModelPortaliC3M930.
SMRiC3M930.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the homogentisate dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F1J. Bacteria.
COG3508. LUCA.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiPOG091H04E0.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen. 1 hit.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.

Sequencei

Sequence statusi: Complete.

C3M930-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGKAEKRSE ALAAEALAYM PGFGNDFETE SLPGALPQGQ NSPQKCNYGL
60 70 80 90 100
YAEQLSGSPF TAPRGTNERS WLYRIRPSVR HTGRFAKIDY PHWKTAPHIA
110 120 130 140 150
EHALALGQLR WNPLPEPTGE LNFLQGIRTM TTAGDVLTQV GMAAHAYVFN
160 170 180 190 200
ADMVDDYFFN ADGELLIVPE MGALQVFTEL GKMDVAPSEI CLVPRGTMFK
210 220 230 240 250
VARLGEEKAW RGYICENYGA KFTLPDRGPI GANCLANPRD FKTPVAAFED
260 270 280 290 300
KETPCRVQVK WCGSFHTVEI GHSPLDVVAW HGNYAPYKYD LKTFSPVGAI
310 320 330 340 350
LFDHPDPSIF TVLTAPSGEE GTANVDFVLF PPRWLVAEHT FRPPWYHRNI
360 370 380 390 400
MSEFMGLIHG RYDAKEEGFV PGGMSLHNMM LAHGPDTSGF EKATNGELKP
410 420 430 440 450
VKLDNTMAFM FETRFPQQLT RFAAELETLQ DDYIDCWAGL RKRFNGTPEG

DWS
Length:453
Mass (Da):50,598
Last modified:June 16, 2009 - v1
Checksum:i036CCDAB72DCBD48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001389 Genomic DNA. Translation: ACP26741.1.
RefSeqiWP_012709494.1. NC_012587.1.
YP_002827494.1. NC_012587.1.

Genome annotation databases

EnsemblBacteriaiACP26741; ACP26741; NGR_c29980.
GeneIDi7792270.
KEGGirhi:NGR_c29980.
PATRICi32312499. VBIRhiSp122450_5838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001389 Genomic DNA. Translation: ACP26741.1.
RefSeqiWP_012709494.1. NC_012587.1.
YP_002827494.1. NC_012587.1.

3D structure databases

ProteinModelPortaliC3M930.
SMRiC3M930.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394.NGR_c29980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP26741; ACP26741; NGR_c29980.
GeneIDi7792270.
KEGGirhi:NGR_c29980.
PATRICi32312499. VBIRhiSp122450_5838.

Phylogenomic databases

eggNOGiENOG4105F1J. Bacteria.
COG3508. LUCA.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiPOG091H04E0.

Enzyme and pathway databases

UniPathwayiUPA00139; UER00339.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen. 1 hit.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHGD_SINFN
AccessioniPrimary (citable) accession number: C3M930
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.