Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Vibrio cholerae serotype O1 (strain M66-2)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotationSAAS annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotationSAAS annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei194Thioimide intermediateUniRule annotation1
Active sitei201Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi95 – 96NADPHUniRule annotation2
Nucleotide bindingi262 – 263NADPHUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotation
Biological processQueuosine biosynthesisUniRule annotationSAAS annotation
LigandNADPUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotationSAAS annotation (EC:1.7.1.13UniRule annotationSAAS annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:VCM66_0859Imported
OrganismiVibrio cholerae serotype O1 (strain M66-2)Imported
Taxonomic identifieri579112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000001217 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliC3LTF1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 135QueF_NInterPro annotationAdd BLAST109

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 95Substrate bindingUniRule annotation3
Regioni233 – 234Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000273755.
KOiK06879.
OMAiQCVERIY.

Family and domain databases

HAMAPiMF_00817. QueF_type2. 1 hit.
InterProiView protein in InterPro
IPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
PfamiView protein in Pfam
PF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.

Sequencei

Sequence statusi: Complete.

C3LTF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRLKNMSKY SDAKELASLT LGKKTEYANQ YDPSLLQPVP RSLNRNDLHL
60 70 80 90 100
SATLPFQGCD IWTLYELSWL NQKGLPQVAI GEVSIPATSA NLIESKSFKL
110 120 130 140 150
YLNSYNQTRF ASWDEVQTRL VHDLSACAGE TVTVNVKSLN EYTAEPIVTM
160 170 180 190 200
QGECIDDQDI EIANYEFDDA LLQGAAQGEE VSEVLHSHLL KSNCLITNQP
210 220 230 240 250
DWGSVEIAYH GAKMNREALL RYLVSFREHN EFHEQCVERI FTDIMRYCQP
260 270 280
QSLTVYARYT RRGGLDINPF RSSHQSAPNH NQRMARQ
Length:287
Mass (Da):32,726
Last modified:June 16, 2009 - v1
Checksum:iC7A848947FAF6A08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001233 Genomic DNA. Translation: ACP05177.1.

Genome annotation databases

EnsemblBacteriaiACP05177; ACP05177; VCM66_0859.
KEGGivcm:VCM66_0859.

Similar proteinsi

Entry informationi

Entry nameiC3LTF1_VIBCM
AccessioniPrimary (citable) accession number: C3LTF1
Entry historyiIntegrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: September 27, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported