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C3LTF1 (C3LTF1_VIBCM) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817

EC=1.7.1.13 HAMAP-Rule MF_00817
Alternative name(s):
7-cyano-7-carbaguanine reductase HAMAP-Rule MF_00817
NADPH-dependent nitrile oxidoreductase HAMAP-Rule MF_00817
PreQ(0) reductase HAMAP-Rule MF_00817
Gene names
Name:queF HAMAP-Rule MF_00817
Ordered Locus Names:VCM66_0859 EMBL ACP05177.1
OrganismVibrio cholerae serotype O1 (strain M66-2) [Complete proteome] [HAMAP] EMBL ACP05177.1
Taxonomic identifier579112 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00817 SAAS SAAS016428

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817 SAAS SAAS016428

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817 SAAS SAAS016428

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00817 SAAS SAAS016428

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00817.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily. HAMAP-Rule MF_00817

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding95 – 962NADPH By similarity HAMAP-Rule MF_00817
Nucleotide binding262 – 2632NADPH By similarity HAMAP-Rule MF_00817
Region93 – 953Substrate binding By similarity HAMAP-Rule MF_00817
Region233 – 2342Substrate binding By similarity HAMAP-Rule MF_00817

Sites

Active site1941Thioimide intermediate By similarity HAMAP-Rule MF_00817
Active site2011Proton donor By similarity HAMAP-Rule MF_00817

Sequences

Sequence LengthMass (Da)Tools
C3LTF1 [UniParc].

Last modified June 16, 2009. Version 1.
Checksum: C7A848947FAF6A08

FASTA28732,726
        10         20         30         40         50         60 
MNRLKNMSKY SDAKELASLT LGKKTEYANQ YDPSLLQPVP RSLNRNDLHL SATLPFQGCD 

        70         80         90        100        110        120 
IWTLYELSWL NQKGLPQVAI GEVSIPATSA NLIESKSFKL YLNSYNQTRF ASWDEVQTRL 

       130        140        150        160        170        180 
VHDLSACAGE TVTVNVKSLN EYTAEPIVTM QGECIDDQDI EIANYEFDDA LLQGAAQGEE 

       190        200        210        220        230        240 
VSEVLHSHLL KSNCLITNQP DWGSVEIAYH GAKMNREALL RYLVSFREHN EFHEQCVERI 

       250        260        270        280 
FTDIMRYCQP QSLTVYARYT RRGGLDINPF RSSHQSAPNH NQRMARQ 

« Hide

References

[1]"A recalibrated molecular clock and independent origins for the cholera pandemic clones."
Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: M66-2 EMBL ACP05177.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001233 Genomic DNA. Translation: ACP05177.1.
RefSeqYP_002809628.1. NC_012578.1.

3D structure databases

ProteinModelPortalC3LTF1.
SMRC3LTF1. Positions 27-287.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING579112.VCM66_0859.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACP05177; ACP05177; VCM66_0859.
GeneID7772138.
KEGGvcm:VCM66_0859.
PATRIC20065515. VBIVibCho108967_0817.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0780.
HOGENOMHOG000273755.
KOK06879.
OMAQCVERIY.
OrthoDBEOG6D5G0M.
ProtClustDBPRK11792.

Enzyme and pathway databases

BioCycVCHO579112:GJAW-916-MONOMER.
UniPathwayUPA00392.

Family and domain databases

HAMAPMF_00817. QueF_type2.
InterProIPR016428. QueF_type2.
[Graphical view]
PIRSFPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsTIGR03138. QueF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC3LTF1_VIBCM
AccessionPrimary (citable) accession number: C3LTF1
Entry history
Integrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: April 16, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)