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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Vibrio cholerae serotype O1 (strain M66-2)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotationSAAS annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei194 – 1941Thioimide intermediateUniRule annotation
Active sitei201 – 2011Proton donorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi95 – 962NADPHUniRule annotation
Nucleotide bindingi262 – 2632NADPHUniRule annotation

GO - Molecular functioni

  1. oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Source: InterPro
  2. preQ1 synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotation

Keywords - Biological processi

Queuosine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

NADPUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciVCHO579112:GJAW-916-MONOMER.
UniPathwayiUPA00392.
UPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotationSAAS annotation (EC:1.7.1.13UniRule annotationSAAS annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:VCM66_0859Imported
OrganismiVibrio cholerae serotype O1 (strain M66-2)Imported
Taxonomic identifieri579112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000001217 Componenti: Chromosome I

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi579112.VCM66_0859.

Structurei

3D structure databases

ProteinModelPortaliC3LTF1.
SMRiC3LTF1. Positions 27-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 953Substrate bindingUniRule annotation
Regioni233 – 2342Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0780.
HOGENOMiHOG000273755.
KOiK06879.
OMAiQCVERIY.
OrthoDBiEOG6D5G0M.

Family and domain databases

HAMAPiMF_00817. QueF_type2.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.

Sequencei

Sequence statusi: Complete.

C3LTF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRLKNMSKY SDAKELASLT LGKKTEYANQ YDPSLLQPVP RSLNRNDLHL
60 70 80 90 100
SATLPFQGCD IWTLYELSWL NQKGLPQVAI GEVSIPATSA NLIESKSFKL
110 120 130 140 150
YLNSYNQTRF ASWDEVQTRL VHDLSACAGE TVTVNVKSLN EYTAEPIVTM
160 170 180 190 200
QGECIDDQDI EIANYEFDDA LLQGAAQGEE VSEVLHSHLL KSNCLITNQP
210 220 230 240 250
DWGSVEIAYH GAKMNREALL RYLVSFREHN EFHEQCVERI FTDIMRYCQP
260 270 280
QSLTVYARYT RRGGLDINPF RSSHQSAPNH NQRMARQ
Length:287
Mass (Da):32,726
Last modified:June 16, 2009 - v1
Checksum:iC7A848947FAF6A08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001233 Genomic DNA. Translation: ACP05177.1.
RefSeqiWP_001880724.1. NC_012578.1.
YP_002809628.1. NC_012578.1.

Genome annotation databases

EnsemblBacteriaiACP05177; ACP05177; VCM66_0859.
KEGGivcm:VCM66_0859.
PATRICi20065515. VBIVibCho108967_0817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001233 Genomic DNA. Translation: ACP05177.1.
RefSeqiWP_001880724.1. NC_012578.1.
YP_002809628.1. NC_012578.1.

3D structure databases

ProteinModelPortaliC3LTF1.
SMRiC3LTF1. Positions 27-287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi579112.VCM66_0859.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP05177; ACP05177; VCM66_0859.
KEGGivcm:VCM66_0859.
PATRICi20065515. VBIVibCho108967_0817.

Phylogenomic databases

eggNOGiCOG0780.
HOGENOMiHOG000273755.
KOiK06879.
OMAiQCVERIY.
OrthoDBiEOG6D5G0M.

Enzyme and pathway databases

UniPathwayiUPA00392.
UPA00392.
BioCyciVCHO579112:GJAW-916-MONOMER.

Family and domain databases

HAMAPiMF_00817. QueF_type2.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A recalibrated molecular clock and independent origins for the cholera pandemic clones."
    Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
    PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: M66-2Imported.

Entry informationi

Entry nameiC3LTF1_VIBCM
AccessioniPrimary (citable) accession number: C3LTF1
Entry historyi
Integrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: April 1, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.