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Protein

Argininosuccinate synthase

Gene

argG

Organism
Vibrio cholerae serotype O1 (strain M66-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei91CitrullineUniRule annotation1
Binding sitei96CitrullineUniRule annotation1
Binding sitei121ATP; via amide nitrogenUniRule annotation1
Binding sitei123AspartateUniRule annotation1
Binding sitei127AspartateUniRule annotation1
Binding sitei127CitrullineUniRule annotation1
Binding sitei128AspartateUniRule annotation1
Binding sitei131CitrullineUniRule annotation1
Binding sitei180CitrullineUniRule annotation1
Binding sitei189CitrullineUniRule annotation1
Binding sitei265CitrullineUniRule annotation1
Binding sitei277CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 20ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:VCM66_2562
OrganismiVibrio cholerae serotype O1 (strain M66-2)
Taxonomic identifieri579112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000001217 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001162951 – 404Argininosuccinate synthaseAdd BLAST404

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC3LRV3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3LRV3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVQVKKVV VAYSGGLDTS VIIPWLKENY DCEVVAFVAD VGQGDEELKG
60 70 80 90 100
VEAKALSSGA SECYIVDLKE EFVKEYIYPT LKTGAYYEGK YLLGTSMARP
110 120 130 140 150
VIAKAQVEIA RKVGADALAH GCTGKGNDQV RFEGAFAALA PDLHVIAPWR
160 170 180 190 200
EWDLRSREAC LDYLAERNIP CAASLTKIYS RDANAWHVST EGGVLESTWN
210 220 230 240 250
APNEDCWVWT VDPEQAPNEA EYVTLQVAHG EVVAVDGEAM TPYNALLYLN
260 270 280 290 300
QKGAKHGVGR IDIVENRLVG MKSRGCYETP GGTIIMEALR AVEQLVLDKT
310 320 330 340 350
SFEFREELGI KASHLVYDGR WFTPLRQAVF AAADELAKDV NGEVVIKLYK
360 370 380 390 400
GQAVATQKRS ANSLYSEDFA TFGADEVYDH SHAGGFIRLY SLSSRIRALS

QNKQ
Length:404
Mass (Da):44,465
Last modified:June 16, 2009 - v1
Checksum:iA65C73B442B5AF82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001233 Genomic DNA. Translation: ACP06858.1.
RefSeqiWP_000049171.1. NC_012578.1.

Genome annotation databases

EnsemblBacteriaiACP06858; ACP06858; VCM66_2562.
KEGGivcm:VCM66_2562.
PATRICi20068846. VBIVibCho108967_2441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001233 Genomic DNA. Translation: ACP06858.1.
RefSeqiWP_000049171.1. NC_012578.1.

3D structure databases

ProteinModelPortaliC3LRV3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP06858; ACP06858; VCM66_2562.
KEGGivcm:VCM66_2562.
PATRICi20068846. VBIVibCho108967_2441.

Phylogenomic databases

HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_VIBCM
AccessioniPrimary (citable) accession number: C3LRV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.