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Protein

Elongation factor Tu

Gene

tufB

Organism
Vibrio cholerae serotype O1 (strain M66-2)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi81 – 855GTPUniRule annotation
Nucleotide bindingi136 – 1394GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-HAMAP
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factorUniRule annotationImported

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciVCHO579112:GJAW-329-MONOMER.
VCHO579112:GJAW-378-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufBImported
Synonyms:tufUniRule annotation, tufAImported
Ordered Locus Names:VCM66_0305Imported, VCM66_0346Imported
OrganismiVibrio cholerae serotype O1 (strain M66-2)Imported
Taxonomic identifieri579112 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000001217: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi579112.VCM66_0346.

Structurei

3D structure databases

ProteinModelPortaliC3LQS5.
SMRiC3LQS5. Positions 4-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
KOiK02358.
OMAiDEGGRHN.
OrthoDBiEOG6R5C6X.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3LQS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKEKFERTK PHVNVGTIGH VDHGKTTLTA AICTVLAKVY GGKARDFASI
60 70 80 90 100
DNAPEERERG ITINTSHVEY DTPNRHYAHV DCPGHADYVK NMITGAAQMD
110 120 130 140 150
GGILVVAATD GPMPQTREHI LLGRQVGIPY IIVFMNKCDM VDDEELLELV
160 170 180 190 200
EMEVRELLSE YDFPGDDLPV IQGSALGALN GEAQWEAKIV ELAEALDTYI
210 220 230 240 250
PEPERAVDMA FLMPIEDVFS IQGRGTVVTG RIERGILKVG DEVAIVGIKE
260 270 280 290 300
TVKTTCTGVE MFRKLLDEGR AGENVGALLR GTKREEVERG QVLAKPGSIT
310 320 330 340 350
PHTKFESEVY VLSKDEGGRH TPFFKGYRPQ FYFRTTDVTG SIELPEGVEM
360 370 380 390
VMPGDNVKMV VDLIAPIAMD EGLRFAIREG GRTVGAGVVA KIIA
Length:394
Mass (Da):43,126
Last modified:June 16, 2009 - v1
Checksum:iBF0DE31A00618B36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001233 Genomic DNA. Translation: ACP04633.1.
CP001233 Genomic DNA. Translation: ACP04674.1.
RefSeqiYP_002809084.1. NC_012578.1.
YP_002809125.1. NC_012578.1.

Genome annotation databases

EnsemblBacteriaiACP04633; ACP04633; VCM66_0305.
ACP04674; ACP04674; VCM66_0346.
GeneIDi7771635.
7774068.
KEGGivcm:VCM66_0305.
vcm:VCM66_0346.
PATRICi20064380. VBIVibCho108967_0289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001233 Genomic DNA. Translation: ACP04633.1.
CP001233 Genomic DNA. Translation: ACP04674.1.
RefSeqiYP_002809084.1. NC_012578.1.
YP_002809125.1. NC_012578.1.

3D structure databases

ProteinModelPortaliC3LQS5.
SMRiC3LQS5. Positions 4-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi579112.VCM66_0346.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP04633; ACP04633; VCM66_0305.
ACP04674; ACP04674; VCM66_0346.
GeneIDi7771635.
7774068.
KEGGivcm:VCM66_0305.
vcm:VCM66_0346.
PATRICi20064380. VBIVibCho108967_0289.

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
KOiK02358.
OMAiDEGGRHN.
OrthoDBiEOG6R5C6X.

Enzyme and pathway databases

BioCyciVCHO579112:GJAW-329-MONOMER.
VCHO579112:GJAW-378-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A recalibrated molecular clock and independent origins for the cholera pandemic clones."
    Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
    PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: M66-2Imported.

Entry informationi

Entry nameiC3LQS5_VIBCM
AccessioniPrimary (citable) accession number: C3LQS5
Entry historyi
Integrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: March 4, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.