C3LAK7 (QUEF_BACAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	BAMEG_3233

Organism

Bacillus anthracis (strain CDC 684 / NRRL 3495) [Complete proteome] [HAMAP]

Taxonomic identifier

568206 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus anthracis

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00818
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00818		PRO_1000148665

Sequences

	Sequence		Length	Mass (Da)
	C3LAK7 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: E08FBCCA7A7FFAE3	FASTA	165	19,528
	10 20 30 40 50 60 MAGRLDEDLK DVTLLGNQNT KYLFEYSPEI LEVFDNNHPN RDYFVKFNCP EFTSLCPKTG 70 80 90 100 110 120 QPDFATIYIS YIPEQRMVES KSLKLYLFSF RNHGDFHEDC MNVIMNDLIK LMDPRYIEVW 130 140 150 160 GKFTPRGGIS IDPYCNYGRP GTKYEQMADY RMMNHDLYPE TIDNR

References

[1]	"Genome sequence of Bacillus anthracis str. CDC 684." Dodson R.J., Munk A.C., Brettin T., Bruce D., Detter C., Tapia R., Han C., Sutton G., Sims D. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 684 / NRRL 3495.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001215 Genomic DNA. Translation: ACP13984.1.
RefSeq	YP_002815819.1. NC_012581.1.
3D structure databases
ProteinModelPortal	C3LAK7.
ModBase	Search...
Protein-protein interaction databases
STRING	568206.BAMEG_3233.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACP13984; ACP13984; BAMEG_3233.
GeneID	7783018.
KEGG	bah:BAMEG_3233.
PATRIC	18796260. VBIBacAnt127120_3506.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HOG000009528.
KO	K09457.
OMA	LECKEFT.
ProtClustDB	PRK13258.
Enzyme and pathway databases
BioCyc	BANT568206:GHVT-3191-MONOMER.
UniPathway	UPA00392.
Family and domain databases
HAMAP	MF_00818. QueF_type1.
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BACAC

Accession

Primary (citable) accession number: C3LAK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 16, 2009
Last modified:	May 29, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program