C3L778 (RNC_BACAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	BAMEG_0644

Organism

Bacillus anthracis (strain CDC 684 / NRRL 3495) [Complete proteome] [HAMAP]

Taxonomic identifier

568206 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus anthracis

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity. HAMAP-Rule MF_00104
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00104
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00104.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_1000118911
Regions
	Domain	19 – 148	130	RNase III
	Domain	174 – 243	70	DRBM
Sites
	Active site	65	1	Potential
	Active site	137	1	By similarity
	Metal binding	61	1	Magnesium By similarity
	Metal binding	134	1	Magnesium By similarity
	Metal binding	137	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	C3L778 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 7249553E3D386DBB	FASTA	245	27,993
	10 20 30 40 50 60 MPYRKYREKK YETKYREAFK LFQEKIGITF TDEKLLIQAF THSSYVNEHR KKPHEDNERL 70 80 90 100 110 120 EFLGDAVLEL TVSQYLFQKY PTMSEGELTK LRAAIVCEPS LVRFANELSF GSLVLLGKGE 130 140 150 160 170 180 EMTGGRERPA LLADVFEAFI GALYLDQGLE TVWGFLKEIV YPKINEGAFS HVMDYKSQLQ 190 200 210 220 230 240 ELIQRDGSGN IEYQILQEKG PAHNREFVSR VTLNNVALGL GSGKSKKEAE QQAAAEALKK LKEQL

References

[1]	"Genome sequence of Bacillus anthracis str. CDC 684." Dodson R.J., Munk A.C., Brettin T., Bruce D., Detter C., Tapia R., Han C., Sutton G., Sims D. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 684 / NRRL 3495.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001215 Genomic DNA. Translation: ACP15956.1.
RefSeq	YP_002813252.1. NC_012581.1.
3D structure databases
ProteinModelPortal	C3L778.
ModBase	Search...
Protein-protein interaction databases
STRING	568206.BAMEG_0644.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACP15956; ACP15956; BAMEG_0644.
GeneID	7782590.
KEGG	bah:BAMEG_0644.
PATRIC	18790994. VBIBacAnt127120_0883.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000246808.
KO	K03685.
OMA	LTHKSCK.
ProtClustDB	PRK00102.
Enzyme and pathway databases
BioCyc	BANT568206:GHVT-620-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_BACAC

Accession

Primary (citable) accession number: C3L778

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 16, 2009
Last modified:	May 29, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program