Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hydroxyethylthiazole kinase 1

Gene

thiM1

Organism
Clostridium botulinum (strain 657 / Type Ba4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421Substrate; via amide nitrogenUniRule annotation
Binding sitei118 – 1181ATPUniRule annotation
Binding sitei164 – 1641ATPUniRule annotation
Binding sitei191 – 1911Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBOT515621:GCP3-508-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinase 1UniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase 1UniRule annotation
Short name:
TH kinase 1UniRule annotation
Short name:
Thz kinase 1UniRule annotation
Gene namesi
Name:thiM1UniRule annotation
Ordered Locus Names:CLJ_B0522
OrganismiClostridium botulinum (strain 657 / Type Ba4)
Taxonomic identifieri515621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002333 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Hydroxyethylthiazole kinase 1PRO_0000383839Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi515621.CLJ_B0522.

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiATGQKDF.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

C3L062-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENKNVIQKM REKTPLIHCI TNYVTINDCA NILLSFGASP AMCEAYDEVY
60 70 80 90 100
DFVSISSALY INLGTLTKEQ ETAAVLASIS AKNHNVPVVI DPVGCPAIKR
110 120 130 140 150
KVEVINRMAE VGRIDIIKGN IGEIKFLAGM DSETRGVDSL DNGENALDAC
160 170 180 190 200
TQLAKKYNCI VAATGEKDFV SDGKRGSVIK NGTEMLTKVT GAGCMLGALC
210 220 230 240 250
AATCANFEDK LVSTTAAILS MNIAGEKAYE KAQLPGSFRI ALIDNIYMIS
260
DEEIWERGNV EWK
Length:263
Mass (Da):28,473
Last modified:June 16, 2009 - v1
Checksum:iCB2C16FB52E36336
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001083 Genomic DNA. Translation: ACQ51920.1.
RefSeqiYP_002861328.1. NC_012658.1.

Genome annotation databases

EnsemblBacteriaiACQ51920; ACQ51920; CLJ_B0522.
KEGGicbi:CLJ_B0522.
PATRICi19409474. VBICloBot19908_0769.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001083 Genomic DNA. Translation: ACQ51920.1.
RefSeqiYP_002861328.1. NC_012658.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi515621.CLJ_B0522.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACQ51920; ACQ51920; CLJ_B0522.
KEGGicbi:CLJ_B0522.
PATRICi19409474. VBICloBot19908_0769.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiATGQKDF.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciCBOT515621:GCP3-508-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Clostridium botulinum Ba4 strain 657."
    Shrivastava S., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 657 / Type Ba4.

Entry informationi

Entry nameiTHIM1_CLOB6
AccessioniPrimary (citable) accession number: C3L062
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: April 1, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.