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Protein

Peptidase T

Gene

pepT

Organism
Clostridium botulinum (strain 657 / Type Ba4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves the N-terminal amino acid of tripeptides.UniRule annotation

Catalytic activityi

Release of the N-terminal residue from a tripeptide.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi78 – 781Zinc 1UniRule annotation
Active sitei80 – 801UniRule annotation
Metal bindingi141 – 1411Zinc 1UniRule annotation
Metal bindingi141 – 1411Zinc 2UniRule annotation
Active sitei175 – 1751Proton acceptorUniRule annotation
Metal bindingi176 – 1761Zinc 2UniRule annotation
Metal bindingi198 – 1981Zinc 1UniRule annotation
Metal bindingi380 – 3801Zinc 2UniRule annotation

GO - Molecular functioni

  1. metallopeptidase activity Source: UniProtKB-KW
  2. tripeptide aminopeptidase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. peptide catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCBOT515621:GCP3-495-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidase TUniRule annotation (EC:3.4.11.4UniRule annotation)
Alternative name(s):
AminotripeptidaseUniRule annotation
Short name:
TripeptidaseUniRule annotation
Tripeptide aminopeptidaseUniRule annotation
Gene namesi
Name:pepTUniRule annotation
Ordered Locus Names:CLJ_B0509
OrganismiClostridium botulinum (strain 657 / Type Ba4)
Taxonomic identifieri515621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002333: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408Peptidase TPRO_1000211989Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi515621.CLJ_B0509.

Structurei

3D structure databases

ProteinModelPortaliC3L049.
SMRiC3L049. Positions 1-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20B family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2195.
HOGENOMiHOG000032390.
KOiK01258.
OMAiYVYATIP.
OrthoDBiEOG6SV59Q.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
HAMAPiMF_00550. Aminopeptidase_M20.
InterProiIPR001261. ArgE/DapE_CS.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
IPR010161. Peptidase_M20B.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF037215. Peptidase_M20B. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01882. peptidase-T. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3L049-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDVLERFLG YIRIDTQSSE ESDTVPTTKT QLEFAKKLGE ELKAIGLKDV
60 70 80 90 100
SVDENGYVMA TLESNIDKKV PTIGFIAHMD TSPDLSGTNI NPRIVEKYDG
110 120 130 140 150
QDIVLNKEKN IVLKINEFPE ILEYKGQDIV VTDGNTLLGA DDKAGIAEII
160 170 180 190 200
TAMEYLINHP EIKHGTIKVG FTPDEEVGKG ADHFDVKKFG ADLAYTLDGG
210 220 230 240 250
GIGELECETF NAAKAKVIIE GRNVHPGSAK NKMTNAVLVA NKFINMLPEN
260 270 280 290 300
EVPERTEGYE GFFHLLSVKS EVETAELNYI IRDFDRKKFE ERKEQIKEVG
310 320 330 340 350
KKINEEYNKE IVCVKVEDQY YNMKEKIDEV KYVVDIAHDA MKAIDIEPIL
360 370 380 390 400
VPIRGGTDGS RLSFMGLPTP NLFAGGHNFH GRFEFVPVLS MEKAAELVVK

IAELYANR
Length:408
Mass (Da):45,624
Last modified:June 16, 2009 - v1
Checksum:i2694F24CE04969AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001083 Genomic DNA. Translation: ACQ54839.1.
RefSeqiWP_003359651.1. NC_012658.1.
YP_002861315.1. NC_012658.1.

Genome annotation databases

EnsemblBacteriaiACQ54839; ACQ54839; CLJ_B0509.
GeneIDi7880723.
KEGGicbi:CLJ_B0509.
PATRICi19409448. VBICloBot19908_0756.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001083 Genomic DNA. Translation: ACQ54839.1.
RefSeqiWP_003359651.1. NC_012658.1.
YP_002861315.1. NC_012658.1.

3D structure databases

ProteinModelPortaliC3L049.
SMRiC3L049. Positions 1-408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi515621.CLJ_B0509.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACQ54839; ACQ54839; CLJ_B0509.
GeneIDi7880723.
KEGGicbi:CLJ_B0509.
PATRICi19409448. VBICloBot19908_0756.

Phylogenomic databases

eggNOGiCOG2195.
HOGENOMiHOG000032390.
KOiK01258.
OMAiYVYATIP.
OrthoDBiEOG6SV59Q.

Enzyme and pathway databases

BioCyciCBOT515621:GCP3-495-MONOMER.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
HAMAPiMF_00550. Aminopeptidase_M20.
InterProiIPR001261. ArgE/DapE_CS.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
IPR010161. Peptidase_M20B.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF037215. Peptidase_M20B. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01882. peptidase-T. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Clostridium botulinum Ba4 strain 657."
    Shrivastava S., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 657 / Type Ba4.

Entry informationi

Entry nameiPEPT_CLOB6
AccessioniPrimary (citable) accession number: C3L049
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: March 4, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.